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[189503_at] choline kinase

Description B0285.8 /REP_DB=WormBase Gene ID /WP=CE00647 /TR=SW:P46558 /GB=CAA84300.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=choline kinase
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 41      4grp_highest_8_8 > 20      4grp_high_8_8 > 31      6grp_highest_nonorm > 71      6grp_total > 41      4grp_total > 0      6grp_highest_sub > 03      6grp_cgc > 82      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 214
1 2 3 4 range
A 320.7
(-1.3)
P 		259.3
(-3)
P 		410
(1.2)
P 		472.8
(3)
P 		214
B 310
(-3)
P 		398.8
(3)
P 		398.9
(3)
P 		380.8
(1.8)
P 		89
C 402.5
(1.4)
P 		363.6
(-3)
P 		416.8
(3)
P 		412.7
(2.5)
P 		53
avr 344.4
(-2.7) 		340.6
(-3) 		408.6
(2) 		422.1
(3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 351
1 2 3 4 5 6 range
A 328.7
(-0.8)
P 		381.8
(0.2)
P 		541.2
(3)
P 		385
(0.2)
P 		307.7
(-1.2)
P 		205.6
(-3)
P 		336
B 373.1
(-0.8)
P 		417
(0.1)
P 		556.7
(3)
P 		418.9
(0.1)
P 		328.7
(-1.7)
P 		266.7
(-3)
P 		290
C 312.2
(-0)
P 		402.1
(3)
P 		357.9
(1.5)
P 		386.6
(2.5)
P 		224.7
(-3)
P 		279.7
(-1.1)
P 		177
avr 338
(-0.8) 		400.3
(0.8) 		485.3
(3) 		396.8
(0.7) 		287
(-2.1) 		250.7
(-3) 		235
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 116.8
(3)
P 		56.3
(-3)
P 		107.2
(2)
P 		69.2
(-1.7)
P 		61
B 98.8
(3)
P 		98.5
(2.9)
P 		92.4
(0.8)
P 		81.1
(-3)
P 		18
C 133.2
(2)
P 		104.5
(-0.2)
P 		145.3
(3)
P 		69.6
(-3)
P 		76
avr 116.3
(3) 		86.4
(-1.2) 		115
(2.8) 		73.3
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 89
1 2 3 4 5 6 range
A 60.6
(-2.3)
P 		135.8
(3)
P 		117.9
(1.7)
P 		97.7
(0.3)
P 		92.8
(-0)
P 		50.1
(-3)
P 		86
B 129.8
(1.6)
P 		138.8
(3)
P 		135.2
(2.5)
P 		110.9
(-1.2)
P 		133.5
(2.2)
P 		99.4
(-3)
P 		39
C 115.7
(2.2)
P 		122.7
(3)
P 		91.1
(-0.8)
P 		77.7
(-2.4)
P 		72.7
(-3)
P 		93.6
(-0.5)
P 		50
avr 102
(-0.5) 		132.4
(3) 		114.7
(0.9) 		95.4
(-1.3) 		99.7
(-0.8) 		81
(-3) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189503_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7776
Sequence Type Exemplar sequence
Representative Public ID B0285.8 NCBI
Target Description B0285.8 /REP_DB=WormBase Gene ID /WP=CE00647 /TR=SW:P46558 /GB=CAA84300.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=choline kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065478(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065478 NCBI Caenorhabditis elegans Choline Kinase B family member (ckb-1) (ckb-1) mRNA, complete cds. 11/11 None
GENEFINDER00000026095 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4364992:4370068:1 10/11 None
SNAP00000026091 ENSEMBL cdna:SNAP chromosome:CEL140:III:4365409:4367360:1 11/11 None
B0285.8 ENSEMBL cdna:known chromosome:CEL140:III:4365409:4367360:1 gene:B0285.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4365937-4367360(+) 100.0 100.0
Public Domain and Genome References
Gene Title choline kinase
Gene Symbol ckb-1
Ensembl B0285.8
Entrez Gene 181904 Entrez gene
SwissProt P46558 EMBL-EBI
WormBase CE33590 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA84300 Hypothetical protein B0285.8 [Caenorhabditis elegans] ref|NP_497879.2| Choline Kinase B family member (ckb-1) [Caenorhabditis elegans] gb|AAN41642.1| choline kinase CKB-1 [Caenorhabditis elegans] sp|P46558|KICB1_CAEEL Choline kinase B1 0.0
blast CAA84301 Hypothetical protein B0285.9 [Caenorhabditis elegans] ref|NP_497880.2| Choline Kinase B family member (ckb-2) [Caenorhabditis elegans] gb|AAN41643.1| choline kinase CKB-2 [Caenorhabditis elegans] sp|P46559|KICB2_CAEEL Choline kinase B2 0.0
blast CAE57506 Hypothetical protein CBG00480 [Caenorhabditis briggsae] 1.0E-173
blast CAA84300 Hypothetical protein B0285.8 [Caenorhabditis elegans] ref|NP_497879.2| Choline Kinase B family member (ckb-1) [Caenorhabditis elegans] gb|AAN41642.1| choline kinase CKB-1 [Caenorhabditis elegans] sp|P46558|KICB1_CAEEL Choline kinase B1 1.0E-151
blast CAE57506 Hypothetical protein CBG00480 [Caenorhabditis briggsae] 1.0E-150
blast CAE57506 Hypothetical protein CBG00480 [Caenorhabditis briggsae] 1.0E-148
blast CAA84301 Hypothetical protein B0285.9 [Caenorhabditis elegans] ref|NP_497880.2| Choline Kinase B family member (ckb-2) [Caenorhabditis elegans] gb|AAN41643.1| choline kinase CKB-2 [Caenorhabditis elegans] sp|P46559|KICB2_CAEEL Choline kinase B2 1.0E-147
Protein Domains
Database ID Description E-Value
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.0E-126
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.6E-37
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.4E-92
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.0E-126
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189503_AT
gatcttggcggtggcgactatgaaataaaaccaacaactgtcacatattcggagcatccc
aagaagatctcaatggatgatttgtacaaggaaatagatttcatggagaaatggacaaat
gaattgtttgaggatactgttgtgttttgtcataatgatctagcatcgagtaacattctc
gagctaaactctacaaaagagctcgtattaattgattgggaattcggatcctacaattgt
agaggttttgatttggcaatgcatttggctgaaactgcggcagattttcgagattcgact
ccaccagggatccgaataagtgaagagcttactgataatccaccgaatcttcaagggttt
tgtgaagcttatgtggatgctgataataagctcaaaaatcgtgtcccatcaaatcgagat
ctggaagtttctaacctgatttgtgaatgtcagttcttttggccaattactcaattattc
tgggcatgcttcgtaatgaaactagcacttttaaagtacaattgtggtgttgatatggat
gttcaagctcaggatcgatttgcggtttact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCTTGGCGGTGGCGACTATGAAA 544 417 337 Antisense
ACTGTCACATATTCGGAGCATCCCA 246 97 373 Antisense
GAGGATACTGTTGTGTTTTGTCATA 646 403 466 Antisense
GTAACATTCTCGAGCTAAACTCTAC 221 461 506 Antisense
AACTGCGGCAGATTTTCGAGATTCG 491 141 609 Antisense
TCGACTCCACCAGGGATCCGAATAA 100 601 631 Antisense
GAAGAGCTTACTGATAATCCACCGA 30 339 658 Antisense
TAAGCTCAAAAATCGTGTCCCATCA 78 633 723 Antisense
GTCAGTTCTTTTGGCCAATTACTCA 169 467 785 Antisense
CTCAATTATTCTGGGCATGCTTCGT 600 227 806 Antisense
GCTCAGGATCGATTTGCGGTTTACT 621 299 883 Antisense
Sequence Source Affymetrix Proprietary Database