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[189494_at] cytochrome P450

Description Y38C9B.1 /REP_DB=WormBase Gene ID /WP=CE24218 /TR=Q9N525 /GB=AAF60505.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_total > 86      4grp_total > 02      6grp_cgc > 67      6grp_cgc_sub > In      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1413
1 2 3 4 range
A 99.1
(-3)
A 		176.7
(-2.6)
A 		197.1
(-2.5)
A 		1234
(3)
P 		1135
B 92.9
(-3)
A 		195.7
(-2.6)
A 		199
(-2.5)
M 		1500.8
(3)
P 		1408
C 98.8
(-3)
A 		149.8
(-2.8)
A 		173.3
(-2.7)
A 		1505.5
(3)
P 		1407
avr 96.9
(-3) 		174.1
(-2.6) 		189.8
(-2.6) 		1413.4
(3) 		1317

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 749
1 2 3 4 5 6 range
A 79.5
(-2.9)
A 		70.9
(-3)
A 		351.1
(-0.5)
M 		471.3
(0.6)
P 		365.1
(-0.4)
P 		745.1
(3)
P 		674
B 105.3
(-2.5)
A 		55.3
(-3)
A 		241.8
(-1.3)
P 		321.8
(-0.6)
P 		421.4
(0.3)
P 		712.6
(3)
P 		657
C 91.8
(-3)
A 		105.5
(-2.9)
A 		288
(-1.3)
M 		454.9
(0.1)
P 		380.4
(-0.6)
P 		804.6
(3)
P 		713
avr 92.2
(-2.9) 		77.2
(-3) 		293.6
(-1.1) 		416
(0) 		389
(-0.2) 		754.1
(3) 		677
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 290
1 2 3 4 range
A 36.1
(-3)
A 		38.3
(-2.9)
A 		51.5
(-2.4)
A 		180.6
(3)
P 		145
B 29.6
(-3)
A 		48.3
(-2.6)
A 		46.1
(-2.7)
M 		319.8
(3)
P 		290
C 32.7
(-3)
A 		43.1
(-2.7)
A 		60.4
(-2.2)
A 		253.8
(3)
P 		221
avr 32.8
(-3) 		43.2
(-2.7) 		52.7
(-2.5) 		251.4
(3) 		219

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 255
1 2 3 4 5 6 range
A 14.7
(-3)
A 		25.2
(-2.6)
A 		76.5
(-0.8)
M 		119.5
(0.8)
P 		110.2
(0.4)
P 		181.4
(3)
P 		167
B 36.7
(-2.6)
A 		18.4
(-3)
A 		58.7
(-2)
P 		85.2
(-1.4)
P 		171.2
(0.7)
P 		265.7
(3)
P 		247
C 34
(-3)
A 		32.2
(-3)
A 		73.3
(-2)
M 		91.5
(-1.5)
P 		123.1
(-0.7)
P 		269.3
(3)
P 		237
avr 28.5
(-2.9) 		25.3
(-3) 		69.5
(-1.8) 		98.7
(-0.9) 		134.8
(0.1) 		238.8
(3) 		214
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189494_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14861
Sequence Type Exemplar sequence
Representative Public ID Y38C9B.1 NCBI
Target Description Y38C9B.1 /REP_DB=WormBase Gene ID /WP=CE24218 /TR=Q9N525 /GB=AAF60505.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070729(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070729 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-29A3) (cyp-29A3) mRNA, complete cds. 11/11 None
SNAP00000016005 ENSEMBL cdna:SNAP chromosome:CEL140:V:60269:63258:1 11/11 None
GENEFINDER00000016009 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:59095:64848:1 11/11 None
Y38C9B.1 ENSEMBL cdna:known chromosome:CEL140:V:59095:63258:1 gene:Y38C9B.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:59094-63258(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-29A3
Ensembl Y38C9B.1
Entrez Gene 189649 Entrez gene
SwissProt Q9N525 EMBL-EBI
WormBase CE35365 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1629875_A_AT Cytochrome P450-4d1 dm
DROSGENOME1:143572_AT Cytochrome P450-4d1 dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_503130 CYtochrome P450 family member (cyp-29A3) [Caenorhabditis elegans] gb|AAF60505.2| Cytochrome p450 family protein 29A3 [Caenorhabditis elegans] 0.0
blast CAE69389 Hypothetical protein CBG15509 [Caenorhabditis briggsae] 0.0
blast CAE69389 Hypothetical protein CBG15509 [Caenorhabditis briggsae] 1.0E-179
blast CAE75191 Hypothetical protein CBG23137 [Caenorhabditis briggsae] 1.0E-164
blast CAE75191 Hypothetical protein CBG23137 [Caenorhabditis briggsae] 1.0E-161
Protein Families
Method ID Description E-Value
p450 pir:A28789 Animalia; CYP1A1/A3; tro; pir:A28789; Trout; A28789 2.0E-90
p450 CP14_CHICK Animalia; CYP1A4; chi; CP14_CHICK; Gallus gallus; P79760 1.0E-112
p450 CP11_ONCMY Animalia; CYP1A2; tro; CP11_ONCMY; Trout; Q92110 1.0E-111
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 9.3E-95
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.3E-85
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503130.2 1 2-20
Y38C9B.1 1 2-20
GENEFINDER00000016009 1 2-20
Sequence
Target Sequence 
>C. ELEGANS:189494_AT
tgaagacctccgccaagaagttgatacatttatgtttgctggacatgatacaacaactac
atctgtgtcctgggtttgttggaatcttgcacatcatccagatattcagcagaacgttta
tgaagagattgtcagtgttttcggagaagatccaaatgaagatgtcactacggagggaat
caagaaattggaatatactgaaagaatgctcaaagagtcgaaaagaatttgcccaactgt
tcccgcggttcttcggcaattgattagtgatatggaaattggtggtgtgctcatcccagc
tggtgcaaacgttgcgatagctccaatggcaattcacaaaaacgcgaatatatatcaaaa
tccagacatatttgatccagatcgtttcctacctgaagaaactgcaaaacgacatgccta
tgactttattcccttttctgcagggcttcggaactgtatcgggcagaagttcgcccagct
aaacgagaaagttatggttatacatcttctgaaaaacttcaaaattgagccaatgggtgg
atattattcaacgaaacaagttttcgagcctgtcggaaagccgtcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAGACCTCCGCCAAGAAGTTGAT 72 575 1086 Antisense
AACAACTACATCTGTGTCCTGGGTT 487 135 1137 Antisense
TCCCGCGGTTCTTCGGCAATTGATT 631 595 1326 Antisense
GAAATTGGTGGTGTGCTCATCCCAG 218 363 1360 Antisense
TCATCCCAGCTGGTGCAAACGTTGC 666 621 1376 Antisense
ATCCAGATCGTTTCCTACCTGAAGA 266 39 1460 Antisense
CGACATGCCTATGACTTTATTCCCT 194 253 1495 Antisense
TTTATTCCCTTTTCTGCAGGGCTTC 233 675 1510 Antisense
GCAGGGCTTCGGAACTGTATCGGGC 437 309 1525 Antisense
GTATCGGGCAGAAGTTCGCCCAGCT 312 455 1541 Antisense
TTCGAGCCTGTCGGAAAGCCGTCTA 241 687 1648 Antisense
Sequence Source Affymetrix Proprietary Database