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[189493_at]

Description D2005.5 /REP_DB=WormBase Gene ID /WP=CE09069 /TR=Q93413 /GB=CAB02082.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=RNA helicase like
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 75      4grp_highest_8_8 > 26      4grp_high_8_8 > 59      6grp_highest_nonorm > 95      6grp_total > 76      4grp_total > 60      6grp_highest_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 286
1 2 3 4 range
A 374.8
(2.1)
P 		389.3
(2.5)
P 		405.4
(3)
P 		194
(-3)
P 		211
B 408.2
(2.3)
P 		352.2
(-0.3)
P 		423
(3)
P 		294
(-3)
P 		129
C 435
(1.3)
P 		367.2
(-1.2)
P 		480.1
(3)
P 		319.6
(-3)
P 		161
avr 406
(1.9) 		369.6
(0.6) 		436.2
(3) 		269.2
(-3) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 744
1 2 3 4 5 6 range
A 537.9
(-2.9)
P 		527.8
(-3)
P 		613.7
(-1.8)
P 		696.2
(-0.6)
P 		651.1
(-1.3)
P 		952.1
(3)
P 		424
B 615.4
(-2.4)
P 		560.2
(-3)
P 		731.5
(-1.2)
P 		652.5
(-2.1)
P 		652.1
(-2.1)
P 		1146.4
(3)
P 		586
C 402
(-3)
P 		595.8
(-1.3)
P 		654.8
(-0.8)
P 		777.8
(0.2)
P 		483.8
(-2.3)
P 		1106.5
(3)
P 		705
avr 518.4
(-3) 		561.3
(-2.5) 		666.7
(-1.4) 		708.8
(-0.9) 		595.7
(-2.2) 		1068.3
(3) 		550
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 139
1 2 3 4 range
A 136.5
(3)
P 		84.5
(0.1)
P 		106
(1.3)
P 		28.4
(-3)
P 		108
B 130.2
(3)
P 		87
(-0.8)
P 		98
(0.1)
P 		62.6
(-3)
P 		68
C 144
(1.8)
P 		105.6
(-0.3)
P 		167.3
(3)
P 		53.9
(-3)
P 		113
avr 136.9
(3) 		92.4
(-0) 		123.8
(2.1) 		48.3
(-3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 328
1 2 3 4 5 6 range
A 99.2
(-3)
P 		187.7
(1)
P 		133.6
(-1.4)
P 		176.6
(0.5)
P 		196.5
(1.4)
P 		231.8
(3)
P 		133
B 214.1
(-2)
P 		186.5
(-2.7)
P 		177.6
(-2.9)
P 		172.8
(-3)
P 		264.9
(-0.8)
P 		427.5
(3)
P 		255
C 149
(-3)
P 		181.8
(-2.1)
P 		166.7
(-2.5)
P 		156.4
(-2.8)
P 		156.6
(-2.8)
P 		370.4
(3)
P 		221
avr 154.1
(-3) 		185.3
(-2) 		159.3
(-2.8) 		168.6
(-2.5) 		206
(-1.4) 		343.2
(3) 		189
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189493_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.918
Sequence Type Exemplar sequence
Representative Public ID D2005.5 NCBI
Target Description D2005.5 /REP_DB=WormBase Gene ID /WP=CE09069 /TR=Q93413 /GB=CAB02082.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=RNA helicase like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059760
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000018871 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7828969:7834366:1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_059760 8/11 Cross Hyb Matching Probes None
SNAP00000018867 8/11 Cross Hyb Matching Probes None
D2005.5 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7828899-7834297(+) 96.01 99.97
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02082 Hypothetical protein D2005.5 [Caenorhabditis elegans] ref|NP_492161.3| D2005.5 [Caenorhabditis elegans] 0.0
blast CAE63741 Hypothetical protein CBG08270 [Caenorhabditis briggsae] 0.0
blast CAE63742 Hypothetical protein CBG08271 [Caenorhabditis briggsae] 2.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.6E-16
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.5E-25
Sequence
Target Sequence 
>C. ELEGANS:189493_AT
agtgttctcgaattacatggtgttcgatccaagtgtatggagatttttgcatgttgaatc
ggttgaaacatttataaagtgcttaaaaattacctggaagtgtcgaattgccgattatca
aattgctgaatttccgaactttgctttccgacaattgactttcaggctatttttgtgcaa
tttccagatgtttcaaaagcgaaaggtgagtaagtacttgagtgaggacaatcaaccact
cagtgatatcaaatgcttccattgcaagttggatgttggaagagcttacaaaataagagg
aacctatttgccgcaactcagtgtcaaggcgttgacatttgttcaagaatcagactattc
ctcgatgacaaaagcaaaatggtcggatgtggaacaggatctgttttatatttcagaagc
aatcgaggatgactttagaatcatgttgaatgcgctctccgatactgaagaaaatatcga
aaagaagattgttcttgatttggactcacgacaacacaataagcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTGTTCTCGAATTACATGGTGTTC 576 59 2823 Antisense
GGAAGTGTCGAATTGCCGATTATCA 39 533 2918 Antisense
TGAATTTCCGAACTTTGCTTTCCGA 706 571 2949 Antisense
GCTTTCCGACAATTGACTTTCAGGC 251 301 2965 Antisense
GAGGACAATCAACCACTCAGTGATA 213 405 3046 Antisense
AAGAGGAACCTATTTGCCGCAACTC 152 153 3117 Antisense
TGCCGCAACTCAGTGTCAAGGCGTT 270 585 3131 Antisense
GAATCAGACTATTCCTCGATGACAA 554 327 3169 Antisense
TCATGTTGAATGCGCTCTCCGATAC 296 621 3263 Antisense
GATTGTTCTTGATTTGGACTCACGA 267 435 3309 Antisense
GGACTCACGACAACACAATAAGCAA 493 525 3324 Antisense
Sequence Source Affymetrix Proprietary Database