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[189488_at] RAS-like protein

Description F20D6.8 /REP_DB=WormBase Gene ID /WP=CE09503 /TR=P90840 /GB=AAB37056.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=RAS-like protein
Cluster 6grp_total > 38      4grp_total > 14      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 730
1 2 3 4 range
A 420.9
(2.1)
P 		131.2
(-1.8)
A 		39.7
(-3)
A 		484.6
(3)
P 		445
B 404
(-0.3)
P 		157.2
(-2.6)
A 		113.3
(-3)
A 		769.8
(3)
P 		657
C 542
(3)
P 		198
(-2.8)
A 		184.7
(-3)
P 		452.5
(1.5)
P 		357
avr 455.6
(1.5) 		162.1
(-2.3) 		112.6
(-3) 		569
(3) 		456

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 246
(0.5)
P 		317.1
(3)
P 		144.3
(-3)
A 		171.5
(-2.1)
P 		173.3
(-2)
P 		239.7
(0.3)
P 		173
B 217.1
(0.8)
P 		276
(3)
A 		132.8
(-2.4)
A 		115.6
(-3)
P 		163.6
(-1.2)
M 		253.5
(2.2)
P 		160
C 318.8
(3)
P 		229.4
(0.1)
P 		166.6
(-1.9)
A 		130.6
(-3)
P 		227.1
(0.1)
P 		234.9
(0.3)
P 		188
avr 260.6
(2.4) 		274.2
(3) 		147.9
(-2.6) 		139.2
(-3) 		188
(-0.8) 		242.7
(1.6) 		135
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 153.2
(3)
P 		28.5
(-2.2)
A 		10.4
(-3)
A 		70.9
(-0.5)
P 		143
B 128.8
(1.5)
P 		38.8
(-2.5)
A 		26.3
(-3)
A 		164
(3)
P 		138
C 179.4
(3)
P 		56.9
(-3)
A 		64.4
(-2.6)
P 		76.3
(-2)
P 		123
avr 153.8
(3) 		41.4
(-2.6) 		33.7
(-3) 		103.7
(0.5) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 45.3
(-2)
P 		112.8
(3)
P 		31.4
(-3)
A 		43.5
(-2.1)
P 		52.3
(-1.5)
P 		58.4
(-1)
P 		81
B 75.5
(1.2)
P 		91.9
(2.8)
A 		32.2
(-2.8)
A 		30.6
(-3)
P 		66.5
(0.4)
M 		94.5
(3)
P 		64
C 118.2
(3)
P 		70
(-0.1)
P 		42.4
(-1.9)
A 		26.3
(-3)
P 		73.5
(0.1)
P 		78.7
(0.4)
P 		92
avr 79.7
(1.8) 		91.6
(3) 		35.3
(-2.8) 		33.5
(-3) 		64.1
(0.2) 		77.2
(1.5) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189488_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11928
Sequence Type Exemplar sequence
Representative Public ID F20D6.8 NCBI
Target Description F20D6.8 /REP_DB=WormBase Gene ID /WP=CE09503 /TR=P90840 /GB=AAB37056.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=RAS-like protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072704(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072704 NCBI Caenorhabditis elegans F20D6.8 (F20D6.8) mRNA, complete cds. 11/11 None
SNAP00000033159 ENSEMBL cdna:SNAP chromosome:CEL140:V:8167866:8169844:-1 11/11 None
GENEFINDER00000033171 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8167866:8170811:-1 11/11 None
F20D6.8 ENSEMBL cdna:known chromosome:CEL140:V:8167351:8170860:-1 gene:F20D6.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8168012-8170812(-) 100.0 100.0
Public Domain and Genome References
Gene Title RAS-like protein
Ensembl F20D6.8
Entrez Gene 184726 Entrez gene
SwissProt P90840 EMBL-EBI
WormBase CE07112 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAM29676 Hypothetical protein F20D6.8 [Caenorhabditis elegans] ref|NP_505105.2| F20D6.8 [Caenorhabditis elegans] 0.0
blast AAM29676 Hypothetical protein F20D6.8 [Caenorhabditis elegans] ref|NP_505105.2| F20D6.8 [Caenorhabditis elegans] 1.0E-173
blast T16117 hypothetical protein F20D6.8 - Caenorhabditis elegans 1.0E-168
blast T16117 hypothetical protein F20D6.8 - Caenorhabditis elegans 1.0E-166
blast CAE66113 Hypothetical protein CBG11333 [Caenorhabditis briggsae] 1.0E-155
blast CAE66113 Hypothetical protein CBG11333 [Caenorhabditis briggsae] 1.0E-153
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 3.1E-14
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 3.1E-14
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 3.1E-14
Sequence
Target Sequence 
>C. ELEGANS:189488_AT
aagaggagataatcgccgtgccacttgcccagatatattcctctttgattcacaaaattg
tctcatgaagcacgttgttctacggatctatggtgcaagaaattgcggaaaaaagagctt
ggccaatcgaatccatcatttcgcaactagcatggcaccggaaagagttgatcccgacga
gaatggaaatgattacactaaaatgacaactttcctgctgaatggaagagaagttacact
ggagattttgctcgaatcgacactggaaaattcaccgttcaggcagtcaaagacactcta
cattgtcatgtacaatatggacaatcgacaatctttcatttatgccacacagattttgga
gagaatcactttggcaaatttgaataatccggtccctcttcaactgtttcttattggaaa
taaatgtgatttgaaacgaaaccaagtgatttccacaaatgaaggaaaatctgtggcgcg
aactttcaaatgtgactttctggaagtatcggctcttcttggaatgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGAGGAGATAATCGCCGTGCCACT 408 149 453 Antisense
GCCCAGATATATTCCTCTTTGATTC 490 287 479 Antisense
GAAGCACGTTGTTCTACGGATCTAT 509 341 519 Antisense
GAGCTTGGCCAATCGAATCCATCAT 420 389 567 Antisense
TTTCGCAACTAGCATGGCACCGGAA 448 665 591 Antisense
ATTCACCGTTCAGGCAGTCAAAGAC 333 9 722 Antisense
GACAATCTTTCATTTATGCCACACA 147 365 779 Antisense
TTTGAATAATCCGGTCCCTCTTCAA 97 669 831 Antisense
TCCCTCTTCAACTGTTTCTTATTGG 520 595 845 Antisense
AAATCTGTGGCGCGAACTTTCAAAT 467 111 919 Antisense
AAGTATCGGCTCTTCTTGGAATGAA 63 157 956 Antisense
Sequence Source Affymetrix Proprietary Database