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[189484_at] cysteine synthase

Description C17G1.7 /REP_DB=WormBase Gene ID /WP=CE08286 /TR=Q93244 /GB=CAB01676.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=cysteine synthase
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 50      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 43      6grp_total > 50      4grp_total > 58      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1096
1 2 3 4 range
A 916
(0.1)
P 		1016.4
(0.8)
P 		1306
(3)
P 		501
(-3)
P 		805
B 904.5
(-0.6)
P 		1363.6
(1.9)
P 		1565
(3)
P 		468.9
(-3)
P 		1096
C 1031.3
(0.4)
P 		1441.4
(3)
P 		1270.6
(1.9)
P 		478.3
(-3)
P 		963
avr 950.6
(0.1) 		1273.8
(2.3) 		1380.5
(3) 		482.7
(-3) 		898

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 482
1 2 3 4 5 6 range
A 850.2
(-3)
P 		1038.5
(-0.3)
P 		1241.4
(2.6)
P 		1268.8
(3)
P 		882
(-2.5)
P 		857.5
(-2.9)
P 		419
B 1158
(1.5)
P 		1027.2
(-0.2)
P 		1272.3
(3)
P 		1252.1
(2.7)
P 		888.8
(-2)
P 		813.6
(-3)
P 		459
C 1226.7
(2.1)
P 		992.9
(-1)
P 		1296
(3)
P 		1289
(2.9)
P 		925.2
(-1.9)
P 		839.9
(-3)
P 		456
avr 1078.3
(0.3) 		1019.5
(-0.5) 		1269.9
(3) 		1270
(3) 		898.7
(-2.1) 		837
(-3) 		433
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 370
1 2 3 4 range
A 333.5
(2.8)
P 		220.6
(0.3)
P 		341.4
(3)
P 		73.3
(-3)
P 		268
B 288.4
(1.3)
P 		336.8
(2.4)
P 		362.7
(3)
P 		99.9
(-3)
P 		263
C 341.4
(1.3)
P 		414.4
(2.5)
P 		442.8
(3)
P 		80.6
(-3)
P 		362
avr 321.1
(1.8) 		323.9
(1.8) 		382.3
(3) 		84.6
(-3) 		298

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 298
1 2 3 4 5 6 range
A 156.7
(-3)
P 		369.3
(3)
P 		270.4
(0.2)
P 		321.8
(1.7)
P 		266.2
(0.1)
P 		208.8
(-1.5)
P 		213
B 402.9
(3)
P 		341.9
(-0.7)
P 		309
(-2.7)
P 		331.6
(-1.3)
P 		361.1
(0.5)
P 		303.4
(-3)
P 		100
C 454.7
(3)
P 		303
(-1.7)
P 		329.9
(-0.8)
P 		259.2
(-3)
P 		299.4
(-1.8)
P 		281.2
(-2.3)
P 		196
avr 338.1
(3) 		338.1
(3) 		303.1
(0.1) 		304.2
(0.2) 		308.9
(0.6) 		264.5
(-3) 		74
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189484_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16952
Sequence Type Exemplar sequence
Representative Public ID C17G1.7 NCBI
Target Description C17G1.7 /REP_DB=WormBase Gene ID /WP=CE08286 /TR=Q93244 /GB=CAB01676.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=cysteine synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077269(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077269 NCBI Caenorhabditis elegans C17G1.7 (C17G1.7) mRNA, complete cds. 11/11 None
SNAP00000020424 ENSEMBL cdna:SNAP chromosome:CEL140:X:9952719:9954463:-1 11/11 None
GENEFINDER00000020425 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9952719:9954463:-1 11/11 None
C17G1.7 ENSEMBL cdna:known chromosome:CEL140:X:9952511:9954472:-1 gene:C17G1.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9952718-9954472(-) 100.0 100.0
Public Domain and Genome References
Gene Title cysteine synthase
Ensembl C17G1.7
Entrez Gene 181209 Entrez gene
SwissProt Q93244 EMBL-EBI
WormBase CE08286 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251487_AT cysteine synthase, mitochondrial, putative / O-acetylserine (thiol)-lyase, putative / O-acetylserine sulfhydrylase, putative at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6520 amino acid metabolism inferred from electronic annotation QuickGO AmiGO
6535 cysteine biosynthesis from serine inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4124 cysteine synthase activity inferred from electronic annotation QuickGO AmiGO
16829 lyase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01676 Hypothetical protein C17G1.7 [Caenorhabditis elegans] ref|NP_509670.1| C17G1.7 [Caenorhabditis elegans] 1.0E-170
blast CAB01676 Hypothetical protein C17G1.7 [Caenorhabditis elegans] ref|NP_509670.1| C17G1.7 [Caenorhabditis elegans] 1.0E-168
blast CAE58761 Hypothetical protein CBG01953 [Caenorhabditis briggsae] 1.0E-142
blast CAE58761 Hypothetical protein CBG01953 [Caenorhabditis briggsae] 1.0E-141
blast XP_469737 cysteine synthase [Oryza sativa (japonica cultivar-group)] gb|AAK71541.1| cysteine synthase [Oryza sativa (japonica cultivar-group)] sp|Q9XEA8|CYSK2_ORYSA Cysteine synthase (O-acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) (CSase) (OAS-TL) gb|AAD23909.1| cysteine synthase [Oryza sativa] 9.0E-90
Protein Domains
Database ID Description E-Value
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 1.0E-126
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189484_AT
acattccagggtctatcattcttgctcaatttgacaacccaaataatccacttgttcact
atcaaacaactggaccagaaatttggagacaaaccaaagggacagtagacgctgttgttt
ttggggttggaactggtggaacaattaccggagttggcagatacttgcaagagcagaatc
caggtgtccgcgtgtttgctgtggagccagaagaatccgctatactcagcggcagaccag
ccggtccacacaaaattcaaggcattggagcagggtttgctccagctgttttggacacaa
agatctatgaagacgtaattcgaattcattccgatgaggctattgtcatggctcaaagac
tttcctatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACATTCCAGGGTCTATCATTCTTGC 122 105 440 Antisense
ATTCTTGCTCAATTTGACAACCCAA 500 11 457 Antisense
CAAATAATCCACTTGTTCACTATCA 573 193 479 Antisense
GTAGACGCTGTTGTTTTTGGGGTTG 106 457 544 Antisense
ATTACCGGAGTTGGCAGATACTTGC 332 11 583 Antisense
AAGAGCAGAATCCAGGTGTCCGCGT 228 153 608 Antisense
GCCAGAAGAATCCGCTATACTCAGC 149 281 645 Antisense
TATACTCAGCGGCAGACCAGCCGGT 173 653 660 Antisense
GGTTTGCTCCAGCTGTTTTGGACAC 596 505 713 Antisense
TCCGATGAGGCTATTGTCATGGCTC 442 593 769 Antisense
GTCATGGCTCAAAGACTTTCCTATG 62 467 784 Antisense
Sequence Source Affymetrix Proprietary Database