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[189479_at]

Description F58E1.6 /REP_DB=WormBase Gene ID /WP=CE11382 /TR=Q9TZH0 /GB=AAC67478.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Na+-H+ exchanger
Cluster 6grp_total > 07      4grp_total > 53      6grp_cgc > 11      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 65.7
(1.7)
A 		79.2
(3)
A 		71
(2.2)
A 		17.5
(-3)
A 		62
B 71.7
(3)
A 		56
(1.6)
A 		34.3
(-0.3)
A 		3.1
(-3)
A 		69
C 84.7
(3)
A 		35.4
(-1)
A 		32.6
(-1.2)
A 		11
(-3)
A 		74
avr 74
(3) 		56.9
(1.4) 		46
(0.4) 		10.5
(-3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 404
1 2 3 4 5 6 range
A 407.4
(3)
P 		149.9
(-1.2)
A 		162.6
(-1)
A 		108.6
(-1.9)
A 		60.3
(-2.7)
A 		43.4
(-3)
A 		364
B 402
(3)
P 		176
(-0.4)
P 		151.8
(-0.8)
A 		106.6
(-1.4)
A 		84
(-1.8)
A 		3.2
(-3)
A 		399
C 390.3
(3)
P 		167.9
(-0.5)
M 		135.6
(-1)
P 		105.3
(-1.4)
A 		69.2
(-2)
A 		5.3
(-3)
A 		385
avr 399.9
(3) 		164.6
(-0.7) 		150
(-0.9) 		106.8
(-1.6) 		71.2
(-2.2) 		17.3
(-3) 		383
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 23.9
(3)
A 		17.2
(1.1)
A 		18.6
(1.5)
A 		2.6
(-3)
A 		21
B 22.9
(3)
A 		13.8
(0.5)
A 		8
(-1)
A 		0.7
(-3)
A 		22
C 28
(3)
A 		10.2
(-1.1)
A 		11.4
(-0.8)
A 		1.9
(-3)
A 		26
avr 24.9
(3) 		13.7
(0.1) 		12.7
(-0.2) 		1.7
(-3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 144
1 2 3 4 5 6 range
A 75.1
(3)
P 		53.3
(1)
A 		35.4
(-0.7)
A 		27.5
(-1.4)
A 		18.2
(-2.3)
A 		10.6
(-3)
A 		65
B 139.9
(3)
P 		58.6
(-0.5)
P 		36.9
(-1.5)
A 		28.2
(-1.8)
A 		34.1
(-1.6)
A 		1.2
(-3)
A 		139
C 144.7
(3)
P 		51.2
(-0.9)
M 		34.5
(-1.6)
P 		21.2
(-2.2)
A 		22.4
(-2.1)
A 		1.8
(-3)
A 		143
avr 119.9
(3) 		54.4
(-0.4) 		35.6
(-1.4) 		25.6
(-1.9) 		24.9
(-1.9) 		4.5
(-3) 		115
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189479_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4487
Sequence Type Exemplar sequence
Representative Public ID F58E1.6 NCBI
Target Description F58E1.6 /REP_DB=WormBase Gene ID /WP=CE11382 /TR=Q9TZH0 /GB=AAC67478.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Na+-H+ exchanger
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027044(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027044 NCBI Caenorhabditis elegans Na/H eXchanger family member (nhx-6) (nhx-6) mRNA, complete cds. 11/11 None
SNAP00000040820 ENSEMBL cdna:SNAP chromosome:CEL140:II:1670864:1674340:1 11/11 None
GENEFINDER00000040839 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1672121:1674340:1 11/11 None
F58E1.6b ENSEMBL cdna:known chromosome:CEL140:II:1670864:1674340:1 gene:F58E1.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027043 6/11 Cross Hyb Matching Probes None
F58E1.6a 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
186511 2 1 NM_001027044 189479_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1670862-1674339(+) 100.0 100.0
Public Domain and Genome References
Ensembl F58E1.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAR30204 Na/h exchanger protein 6, isoform b [Caenorhabditis elegans] ref|NP_001022215.1| Na/H eXchanger family member (nhx-6) [Caenorhabditis elegans] 0.0
blast AAC67478 Na/h exchanger protein 6, isoform a [Caenorhabditis elegans] ref|NP_001022214.1| Na/H eXchanger family member (nhx-6) [Caenorhabditis elegans] 0.0
blast AAM18108 putative Na-H exchanger isoform 6 [Caenorhabditis elegans] 0.0
blast CAE67516 Hypothetical protein CBG13035 [Caenorhabditis briggsae] 0.0
blast AAA62524 Na/h exchanger protein 2 [Caenorhabditis elegans] ref|NP_495614.3| Na/H eXchanger family member (nhx-2) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006153 EMBL-EBI Sodium/hydrogen exchanger 1.0E-126
Pfam IPR006153 EMBL-EBI Sodium/hydrogen exchanger 6.0E-66
Pfam IPR006153 EMBL-EBI Sodium/hydrogen exchanger 1.2E-22
Pfam IPR006153 EMBL-EBI Sodium/hydrogen exchanger 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022215.1 11 7-24,76-98,110-129,139-161,173-195,205-227,247-269,284-315,335-357,367-389,432-451
F58E1.6b 11 7-24,76-98,110-129,139-161,173-195,205-227,247-269,284-315,335-357,367-389,432-451
SNAP00000040820 9 7-24,49-71,84-106,116-138,158-180,190-224,245-267,277-299,311-330
GENEFINDER00000040839 10 23-40,52-71,81-103,115-137,147-169,189-211,226-257,277-299,309-331,374-393
Sequence
Target Sequence 
>C. ELEGANS:189479_AT
gtaatatacttcactgtcttccttcagggcataaccctgaagcctatcgccgagtttctg
caagttgagaagaaaaacgttcactctaagaacatgatcgagcatatttacaccgagctg
atcgacacaacgatggccgggatggaggatattgcaggattcaagggacatcactggatc
cgcgattcatggaattactttaacaattgctacctgcggccggtgctggtgaataagaac
aatatgaagcagatggacaagacgaagttggtcaggaagtataagcacctcgtcgacgaa
gacgctaagaaaattgccaatggcggggatctcaagagcaacttggcattcactagagca
ctcatcgagcacaccagaagccggaccaatactgcgataactagcgactcaaatgggaaa
atcgattttgtgacgcatatgaaggaacatcatgggatcactgtctac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAATATACTTCACTGTCTTCCTTC 391 463 1327 Antisense
CCTATCGCCGAGTTTCTGCAAGTTG 443 269 1369 Antisense
TCGACACAACGATGGCCGGGATGGA 590 601 1448 Antisense
AAGGGACATCACTGGATCCGCGATT 337 159 1489 Antisense
ACTTTAACAATTGCTACCTGCGGCC 584 99 1523 Antisense
CTGCGGCCGGTGCTGGTGAATAAGA 409 229 1540 Antisense
AAGCACCTCGTCGACGAAGACGCTA 477 151 1609 Antisense
GCCAATGGCGGGGATCTCAAGAGCA 265 277 1642 Antisense
ACTTGGCATTCACTAGAGCACTCAT 64 105 1667 Antisense
GAAGCCGGACCAATACTGCGATAAC 238 343 1703 Antisense
GGAACATCATGGGATCACTGTCTAC 285 529 1770 Antisense
Sequence Source Affymetrix Proprietary Database