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[189474_at] carboxylesterase

Description F13H6.4 /REP_DB=WormBase Gene ID /WP=CE09376 /TR=O16352 /GB=AAB65885.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=carboxylesterase
Cluster 6grp_total > 70      4grp_total > 20      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 22.2
(-2.5)
A 		18.6
(-2.9)
A 		17.7
(-3)
A 		75.2
(3)
P 		58
B 33.1
(3)
A 		29.2
(2.2)
P 		3.8
(-3)
A 		26.4
(1.6)
A 		29
C 42
(0.8)
P 		34.9
(-0.1)
M 		11.9
(-3)
A 		59
(3)
A 		47
avr 32.4
(0) 		27.6
(-0.7) 		11.1
(-3) 		53.5
(3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 25.3
(0.6)
A 		11.6
(-3)
A 		26.3
(0.9)
A 		34.5
(3)
P 		26.7
(1)
P 		32.1
(2.4)
P 		23
B 22.9
(-1.4)
A 		37.9
(2.8)
P 		19.1
(-2.4)
A 		38.7
(3)
P 		20.2
(-2.1)
A 		17.1
(-3)
A 		22
C 14.6
(-1.6)
A 		30.9
(0.4)
A 		25.9
(-0.2)
P 		52.8
(3)
P 		2.8
(-3)
A 		17
(-1.3)
A 		50
avr 20.9
(-2) 		26.8
(-0.6) 		23.8
(-1.3) 		42
(3) 		16.6
(-3) 		22.1
(-1.7) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 8.1
(0.5)
A 		4
(-3)
A 		4.6
(-2.5)
A 		11
(3)
P 		7
B 10.6
(3)
A 		7.2
(0.9)
P 		0.9
(-3)
A 		5.6
(-0.1)
A 		10
C 13.9
(3)
P 		10
(0.6)
M 		4.1
(-3)
A 		10
(0.6)
A 		10
avr 10.9
(3) 		7.1
(0) 		3.2
(-3) 		8.9
(1.4) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 4.7
(-2.2)
A 		4.1
(-3)
A 		5.7
(-0.9)
A 		8.7
(3)
P 		8
(2.1)
P 		7.8
(1.8)
P 		5
B 8
(-0.5)
A 		12.6
(3)
P 		4.6
(-3)
A 		10.3
(1.3)
P 		8.2
(-0.3)
A 		6.4
(-1.7)
A 		8
C 5.4
(-0.2)
A 		9.4
(2.3)
A 		6.6
(0.5)
P 		10.6
(3)
P 		0.9
(-3)
A 		5.7
(-0)
A 		10
avr 6
(-2.4) 		8.7
(1.3) 		5.6
(-3) 		9.9
(3) 		5.7
(-2.9) 		6.6
(-1.6) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189474_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12210
Sequence Type Exemplar sequence
Representative Public ID F13H6.4 NCBI
Target Description F13H6.4 /REP_DB=WormBase Gene ID /WP=CE09376 /TR=O16352 /GB=AAB65885.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=carboxylesterase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072221(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072221 NCBI Caenorhabditis elegans F13H6.4 (carboxylesterase) mRNA, complete cds. 11/11 None
SNAP00000034393 ENSEMBL cdna:SNAP chromosome:CEL140:V:6369349:6371127:-1 9/11 None
GENEFINDER00000034397 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6368356:6369946:-1 10/11 None
F13H6.4 ENSEMBL cdna:known chromosome:CEL140:V:6369027:6371103:-1 gene:F13H6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6369026-6371127(-) 100.0 100.0
Public Domain and Genome References
Gene Title carboxylesterase
Ensembl F13H6.4
Entrez Gene 184435 Entrez gene
SwissProt O16352 EMBL-EBI
WormBase CE35857 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB65885 Hypothetical protein F13H6.4 [Caenorhabditis elegans] ref|NP_504622.2| F13H6.4 [Caenorhabditis elegans] 0.0
blast AAB65885 Hypothetical protein F13H6.4 [Caenorhabditis elegans] ref|NP_504622.2| F13H6.4 [Caenorhabditis elegans] 1.0E-179
blast AAB65887 Hypothetical protein F13H6.3 [Caenorhabditis elegans] ref|NP_504621.1| F13H6.3 [Caenorhabditis elegans] 1.0E-170
blast CAA46899 esterase [Caenorhabditis elegans] sp|Q07085|EST2_CAEEL Esterase CM06B1 1.0E-166
blast CAA46899 esterase [Caenorhabditis elegans] sp|Q07085|EST2_CAEEL Esterase CM06B1 1.0E-152
blast AAB65887 Hypothetical protein F13H6.3 [Caenorhabditis elegans] ref|NP_504621.1| F13H6.3 [Caenorhabditis elegans] 1.0E-152
blast AAB65885 Hypothetical protein F13H6.4 [Caenorhabditis elegans] ref|NP_504622.2| F13H6.4 [Caenorhabditis elegans] 1.0E-126
Protein Domains
Database ID Description E-Value
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.3E-86
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 5.7E-39
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 0.0015
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 4.4E-68
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 7.8E-12
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 7.5E-28
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.3E-40
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000034397 1 270-292
Sequence
Target Sequence 
>C. ELEGANS:189474_AT
gactttgcgaagcttttggagatcttggattcaatcttggagtgttccagtcagccaaga
gttcagctaaatatgggaatgatgtgttcttgtattcatttgaatattatcattcagatg
gttttgggatgtggaaagatcttctgccattcgaagcttctatgcatggaacagagctcc
gttatttgctcggcgaaggattctacagcaaatttgatgcaacgaaggaggaactggagg
ttcttgaaaaaacaaccacacttttttcaaattttgctaaatacggaaatccgaatggaa
cgggagttacggatgaaatttgggagaagtttaacttgaacaacccagaaagacattttc
gaatttcgtatccaaaatgtgaaatgagagatatttttagtggtgggagaatgccgtttt
tggagaagctcgaagaaaaaagcttgaagacccaggagctggtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTTTGCGAAGCTTTTGGAGATCT 61 373 1034 Antisense
GATTCAATCTTGGAGTGTTCCAGTC 149 431 1061 Antisense
TGTTCCAGTCAGCCAAGAGTTCAGC 458 563 1076 Antisense
GATGTGTTCTTGTATTCATTTGAAT 304 409 1114 Antisense
GATGTGGAAAGATCTTCTGCCATTC 24 409 1161 Antisense
TTCTGCCATTCGAAGCTTCTATGCA 121 695 1175 Antisense
GCTTCTATGCATGGAACAGAGCTCC 678 305 1189 Antisense
ACAGAGCTCCGTTATTTGCTCGGCG 237 109 1204 Antisense
GCTCGGCGAAGGATTCTACAGCAAA 683 293 1221 Antisense
GAATGCCGTTTTTGGAGAAGCTCGA 436 329 1442 Antisense
AAGCTTGAAGACCCAGGAGCTGGTG 477 149 1473 Antisense
Sequence Source Affymetrix Proprietary Database