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[189470_at]

Description R74.5 /REP_DB=WormBase Gene ID /WP=CE01058 /TR=Q22030 /GB=CAA85276.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=RNA binding protein
Cluster 6grp_high1_10_10 > 28      6grp_total > 27      4grp_total > 19      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 306
1 2 3 4 range
A 446.3
(0.3)
M 		516.7
(3)
A 		358.9
(-3)
A 		488.5
(1.9)
M 		158
B 491
(-0.7)
P 		381.4
(-3)
A 		379.8
(-3)
A 		664.9
(3)
P 		285
C 407.9
(-2.5)
A 		536.5
(0.4)
A 		383.2
(-3)
M 		657.1
(3)
P 		274
avr 448.4
(-1.1) 		478.2
(-0.3) 		374
(-3) 		603.5
(3) 		230

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 214
1 2 3 4 5 6 range
A 506.6
(3)
P 		400.3
(-0.3)
P 		315.1
(-3)
A 		351.2
(-1.9)
P 		393
(-0.6)
P 		427.5
(0.5)
P 		192
B 509.9
(3)
P 		455.9
(1.4)
P 		330.3
(-2.2)
P 		301.1
(-3)
P 		341.5
(-1.8)
P 		346.1
(-1.7)
P 		209
C 515.5
(3)
P 		427
(-1.7)
P 		440.7
(-1)
P 		403.6
(-3)
P 		403.9
(-3)
P 		424.6
(-1.9)
P 		112
avr 510.7
(3) 		427.7
(-0.1) 		362
(-2.6) 		352
(-3) 		379.5
(-2) 		399.4
(-1.2) 		159
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 91
1 2 3 4 range
A 162.5
(3)
M 		112.2
(-0.3)
A 		93.8
(-1.5)
A 		71.5
(-3)
M 		91
B 156.6
(3)
P 		94.2
(-2.5)
A 		88
(-3)
A 		141.7
(1.7)
P 		69
C 135
(0.3)
A 		154.3
(3)
A 		133.6
(0.1)
M 		110.8
(-3)
P 		44
avr 151.4
(3) 		120.2
(-1) 		105.1
(-3) 		108
(-2.6) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 93.4
(-1)
P 		142.4
(3)
P 		68.6
(-3)
A 		89.1
(-1.3)
P 		118.6
(1.1)
P 		104.1
(-0.1)
P 		74
B 177.4
(3)
P 		151.8
(1.4)
P 		80.2
(-3)
P 		79.7
(-3)
P 		138.8
(0.6)
P 		129.1
(0)
P 		98
C 191.1
(3)
P 		130.3
(-0.3)
P 		112.2
(-1.3)
P 		81.2
(-3)
P 		130.7
(-0.3)
P 		142.1
(0.3)
P 		110
avr 154
(3) 		141.5
(1.9) 		87
(-2.7) 		83.3
(-3) 		129.4
(0.9) 		125.1
(0.5) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189470_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7133
Sequence Type Exemplar sequence
Representative Public ID R74.5 NCBI
Target Description R74.5 /REP_DB=WormBase Gene ID /WP=CE01058 /TR=Q22030 /GB=CAA85276.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=RNA binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065440(11), NM_181938(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065440 NCBI Caenorhabditis elegans R74.5a (R74.5) mRNA, complete cds. 11/11 None
NM_181938 NCBI Caenorhabditis elegans R74.5b (R74.5) mRNA, complete cds. 11/11 None
GENEFINDER00000044756 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4197269:4201796:1 11/11 None
SNAP00000044750 ENSEMBL cdna:SNAP chromosome:CEL140:III:4198885:4201796:1 11/11 None
R74.5a.2 ENSEMBL cdna:known chromosome:CEL140:III:4197254:4202260:1 gene:R74.5 11/11 None
R74.5b.1 ENSEMBL cdna:known chromosome:CEL140:III:4197256:4201796:1 gene:R74.5 11/11 None
R74.5a.1 ENSEMBL cdna:known chromosome:CEL140:III:4197256:4202260:1 gene:R74.5 11/11 None
R74.5b.3 ENSEMBL cdna:known chromosome:CEL140:III:4197256:4201796:1 gene:R74.5 11/11 None
R74.5b.5 ENSEMBL cdna:known chromosome:CEL140:III:4197261:4201796:1 gene:R74.5 11/11 None
R74.5b.2 ENSEMBL cdna:known chromosome:CEL140:III:4198250:4201796:1 gene:R74.5 11/11 None
R74.5b.4 ENSEMBL cdna:known chromosome:CEL140:III:4199955:4201796:1 gene:R74.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4197268-4201796(+) 100.0 100.0
Public Domain and Genome References
Ensembl R74.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA85276 Hypothetical protein R74.5a [Caenorhabditis elegans] ref|NP_497841.1| R74.5a [Caenorhabditis elegans] 1.0E-130
blast CAA85276 Hypothetical protein R74.5a [Caenorhabditis elegans] ref|NP_497841.1| R74.5a [Caenorhabditis elegans] 1.0E-119
blast CAA85276 Hypothetical protein R74.5a [Caenorhabditis elegans] ref|NP_497841.1| R74.5a [Caenorhabditis elegans] 2.0E-99
blast CAD82915 Hypothetical protein R74.5b [Caenorhabditis elegans] ref|NP_871667.1| R74.5b [Caenorhabditis elegans] 5.0E-84
blast CAA85276 Hypothetical protein R74.5a [Caenorhabditis elegans] ref|NP_497841.1| R74.5a [Caenorhabditis elegans] 5.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR000504 EMBL-EBI RNA-binding region RNP-1 (RNA recognition motif) 1.4E-19
Pfam IPR000504 EMBL-EBI RNA-binding region RNP-1 (RNA recognition motif) 7.2E-15
Pfam IPR000504 EMBL-EBI RNA-binding region RNP-1 (RNA recognition motif) 1.4E-19
Pfam IPR000504 EMBL-EBI RNA-binding region RNP-1 (RNA recognition motif) 1.4E-19
Sequence
Target Sequence 
>C. ELEGANS:189470_AT
aatgctctgatatcccaacaacaacagattcaacgtgctgctcttctgcaacagcaactt
cttgctcaacaattccttgtgccacgtcatcaacaactcatgatgccaccaacttctgca
gcagccatctctcaactgcaagctcttcaatatcaacaggcactgctcgctgctcaacaa
caacagcaacaaatgcagaatcctcaattccttttaagtcttcaacaacagcaacaggca
caagcacaggctccaccgacatcgatggctcagcttattcaacatcaacaacagtttgca
atggatccgatggctcaggcacaggcacaagctgctcaagcacaaatggtcacgttgatt
gcatttgagcaacagagaatgcaacttcaagctgctgctgccgctgctcaaggtcgaggt
gttcctcagccagggcgcattccaacatcaacagccaccaccggatcatcacaaaatcaa
gtcgctgcaggctcaattggagagcaatacttacaacaatcaatgcaaggagcagttcca
gcaaatttccatcatcactcacaggct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGCTCTGATATCCCAACAACAAC 5 167 604 Antisense
GCTCTTCTGCAACAGCAACTTCTTG 370 301 643 Antisense
CTTGCTCAACAATTCCTTGTGCCAC 311 219 664 Antisense
AGCAGCCATCTCTCAACTGCAAGCT 346 77 723 Antisense
CAATATCAACAGGCACTGCTCGCTG 355 181 751 Antisense
GGCTCAGCTTATTCAACATCAACAA 476 539 870 Antisense
AATGCAACTTCAAGCTGCTGCTGCC 655 165 981 Antisense
CAAGGTCGAGGTGTTCCTCAGCCAG 87 183 1012 Antisense
TCAAGTCGCTGCAGGCTCAATTGGA 606 631 1080 Antisense
GAGCAGTTCCAGCAAATTTCCATCA 477 389 1133 Antisense
AAATTTCCATCATCACTCACAGGCT 222 117 1146 Antisense
Sequence Source Affymetrix Proprietary Database