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[189460_at] choline kinase

Description B0285.9 /REP_DB=WormBase Gene ID /WP=CE00648 /TR=SW:P46559 /GB=CAA84301.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=choline kinase
Cluster 6grp_highest_10_10 > 01      6grp_high1_10_10 > 07      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 41      6grp_total > 09      4grp_total > 60      6grp_cgc > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1354
1 2 3 4 range
A 1257.9
(2.2)
P 		1023.1
(1)
P 		1418.3
(3)
P 		249.2
(-3)
P 		1169
B 1401.7
(2.3)
P 		1222.5
(1.5)
P 		1564.8
(3)
P 		210.6
(-3)
P 		1354
C 1277.8
(2.2)
P 		1189.8
(1.8)
P 		1440
(3)
P 		224.3
(-3)
P 		1216
avr 1312.5
(2.2) 		1145.1
(1.4) 		1474.4
(3) 		228
(-3) 		1246

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 523
1 2 3 4 5 6 range
A 1081.9
(3)
P 		612.6
(-2.5)
P 		710.9
(-1.3)
P 		823.5
(-0)
P 		583
(-2.8)
P 		568.2
(-3)
P 		514
B 1013.2
(3)
P 		595.2
(-2.5)
P 		579.4
(-2.7)
P 		746.2
(-0.5)
P 		697.4
(-1.2)
P 		558.5
(-3)
P 		455
C 875.9
(3)
P 		694.6
(-1)
P 		752.3
(0.2)
P 		770.9
(0.7)
P 		607.3
(-3)
P 		627.1
(-2.6)
P 		269
avr 990.3
(3) 		634.1
(-2.3) 		680.9
(-1.6) 		780.2
(-0.1) 		629.2
(-2.3) 		584.6
(-3) 		406
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 465
1 2 3 4 range
A 458
(3)
P 		222.1
(-0.4)
P 		370.8
(1.8)
P 		36.5
(-3)
P 		422
B 447
(3)
P 		301.9
(0.8)
P 		362.6
(1.7)
P 		44.9
(-3)
P 		402
C 423
(2)
P 		342.1
(0.9)
P 		501.9
(3)
P 		37.8
(-3)
P 		464
avr 442.7
(3) 		288.7
(0.7) 		411.8
(2.5) 		39.7
(-3) 		403

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 214
1 2 3 4 5 6 range
A 199.4
(1.6)
P 		217.9
(3)
P 		154.8
(-1.8)
P 		208.9
(2.3)
P 		175.9
(-0.2)
P 		138.3
(-3)
P 		80
B 352.5
(3)
P 		198.1
(-1.4)
P 		140.7
(-3)
P 		197.6
(-1.4)
P 		283.3
(1)
P 		208.3
(-1.1)
P 		212
C 324.6
(3)
P 		211.9
(-1)
P 		191.5
(-1.7)
P 		155
(-3)
P 		196.5
(-1.5)
P 		209.9
(-1.1)
P 		170
avr 292.2
(3) 		209.3
(-0.8) 		162.3
(-3) 		187.2
(-1.8) 		218.6
(-0.4) 		185.5
(-1.9) 		130
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189460_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7877
Sequence Type Exemplar sequence
Representative Public ID B0285.9 NCBI
Target Description B0285.9 /REP_DB=WormBase Gene ID /WP=CE00648 /TR=SW:P46559 /GB=CAA84301.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=choline kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065479(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065479 NCBI Caenorhabditis elegans Choline Kinase B family member (ckb-2) (ckb-2) mRNA, complete cds. 11/11 None
GENEFINDER00000026095 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4364992:4370068:1 11/11 None
SNAP00000026092 ENSEMBL cdna:SNAP chromosome:CEL140:III:4367945:4370068:1 11/11 None
B0285.9 ENSEMBL cdna:known chromosome:CEL140:III:4367945:4370068:1 gene:B0285.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4368203-4370068(+) 100.0 100.0
Public Domain and Genome References
Gene Title choline kinase
Gene Symbol ckb-2
Ensembl B0285.9
Entrez Gene 175565 Entrez gene
SwissProt P46559 EMBL-EBI
WormBase CE33591 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA84301 Hypothetical protein B0285.9 [Caenorhabditis elegans] ref|NP_497880.2| Choline Kinase B family member (ckb-2) [Caenorhabditis elegans] gb|AAN41643.1| choline kinase CKB-2 [Caenorhabditis elegans] sp|P46559|KICB2_CAEEL Choline kinase B2 0.0
blast CAE57506 Hypothetical protein CBG00480 [Caenorhabditis briggsae] 1.0E-174
blast CAE57506 Hypothetical protein CBG00480 [Caenorhabditis briggsae] 1.0E-173
blast CAA84300 Hypothetical protein B0285.8 [Caenorhabditis elegans] ref|NP_497879.2| Choline Kinase B family member (ckb-1) [Caenorhabditis elegans] gb|AAN41642.1| choline kinase CKB-1 [Caenorhabditis elegans] sp|P46558|KICB1_CAEEL Choline kinase B1 1.0E-152
Protein Domains
Database ID Description E-Value
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.0E-126
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.6E-37
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.4E-92
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189460_AT
tacagtcaactattcggatcatcctaaatcagtttccatcaaagatctgaatcatgaaat
cgacaatttcgaaaaatggtcgactgaaatttttgagcacactttggtcttcagtcacaa
tgatctagcgtctaccaacattctcgagctcaactccacaaaagagctcgtattaattga
ttgggaatttggaacttataactggagaggatttgatttggcaatgcatttatccgagac
agcgattgattatcgtgtaccatttccacctggaatcaagatgaatggagatttaattga
taatccaccaaatattcaaattttctgtgaagcttatgttgaagctgacaaaaagctcaa
aaaccgttcaccatcggatccaactgctgaagtgaaagctctaattcaagaatgtcaatt
cttctggcctcttacaaatctcttctgggcactctcagccatgaagcactcacttcttaa
attcgaaaatggagttgatttggacgtgcaagcacgtgatcgattggcagtttacttcca
cttgaaacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACAGTCAACTATTCGGATCATCCT 496 643 372 Antisense
GGATCATCCTAAATCAGTTTCCATC 327 511 387 Antisense
GCACACTTTGGTCTTCAGTCACAAT 229 317 468 Antisense
CAGTCACAATGATCTAGCGTCTACC 364 207 483 Antisense
TAGCGTCTACCAACATTCTCGAGCT 385 651 497 Antisense
CATTCTCGAGCTCAACTCCACAAAA 211 217 510 Antisense
TATCGTGTACCATTTCCACCTGGAA 433 659 622 Antisense
AAAGCTCAAAAACCGTTCACCATCG 535 123 723 Antisense
TCACCATCGGATCCAACTGCTGAAG 707 625 739 Antisense
TTCTGGGCACTCTCAGCCATGAAGC 21 695 814 Antisense
GGCAGTTTACTTCCACTTGAAACCA 565 533 897 Antisense
Sequence Source Affymetrix Proprietary Database