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[189459_at] transport protein

Description F32H5.4 /REP_DB=WormBase Gene ID /WP=CE09888 /TR=P91993 /GB=CAB04250.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=transport protein
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 03      4grp_highest_8_8 > 60      4grp_high_8_8 > 25      6grp_highest_nonorm > 70      6grp_total > 14      4grp_total > 39      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 288
1 2 3 4 range
A 450.6
(3)
P 		313.4
(-0.6)
P 		219.4
(-3)
P 		225.8
(-2.8)
P 		231
B 499.8
(3)
P 		211.7
(-3)
P 		234.2
(-2.5)
P 		337.1
(-0.4)
P 		288
C 457.1
(3)
P 		244.9
(-2.3)
P 		216.4
(-3)
P 		357
(0.5)
P 		241
avr 469.2
(3) 		256.7
(-2.2) 		223.3
(-3) 		306.6
(-1) 		246

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 425
1 2 3 4 5 6 range
A 554.9
(3)
P 		363.3
(0.2)
P 		358.3
(0.1)
P 		254.9
(-1.5)
P 		196.4
(-2.3)
P 		151.2
(-3)
P 		404
B 371.3
(3)
P 		370
(3)
P 		265.4
(0.4)
P 		259
(0.2)
P 		200.7
(-1.2)
P 		129.7
(-3)
P 		242
C 401.9
(3)
P 		398.7
(2.9)
P 		315.8
(0.6)
P 		316.3
(0.6)
P 		183.7
(-3)
P 		193.6
(-2.7)
P 		218
avr 442.7
(3) 		377.3
(1.6) 		313.2
(0.3) 		276.7
(-0.5) 		193.6
(-2.3) 		158.2
(-3) 		285
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 131
1 2 3 4 range
A 164.1
(3)
P 		68
(-1.4)
P 		57.4
(-1.9)
P 		33.1
(-3)
P 		131
B 159.4
(3)
P 		52.3
(-3)
P 		54.3
(-2.9)
P 		71.8
(-1.9)
P 		107
C 151.3
(3)
P 		70.4
(-2.3)
P 		75.4
(-2)
P 		60.2
(-3)
P 		91
avr 158.3
(3) 		63.6
(-2.5) 		62.4
(-2.6) 		55
(-3) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 102.3
(1.3)
P 		129.2
(3)
P 		78
(-0.3)
P 		64.7
(-1.2)
P 		59.3
(-1.5)
P 		36.8
(-3)
P 		92
B 129.2
(3)
P 		123.1
(2.5)
P 		64.4
(-1.8)
P 		68.6
(-1.5)
P 		81.6
(-0.5)
P 		48.4
(-3)
P 		81
C 149
(3)
P 		121.7
(1.2)
P 		80.4
(-1.6)
P 		63.6
(-2.7)
P 		59.4
(-3)
P 		64.8
(-2.6)
P 		90
avr 126.8
(3) 		124.7
(2.8) 		74.3
(-1.1) 		65.6
(-1.8) 		66.8
(-1.7) 		50
(-3) 		77
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189459_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13438
Sequence Type Exemplar sequence
Representative Public ID F32H5.4 NCBI
Target Description F32H5.4 /REP_DB=WormBase Gene ID /WP=CE09888 /TR=P91993 /GB=CAB04250.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=transport protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073912(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073912 NCBI Caenorhabditis elegans F32H5.4 (F32H5.4) mRNA, complete cds. 11/11 None
SNAP00000043410 ENSEMBL cdna:SNAP chromosome:CEL140:V:13363796:13365881:1 11/11 None
GENEFINDER00000043415 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13363796:13365881:1 11/11 None
F32H5.4 ENSEMBL cdna:known chromosome:CEL140:V:13363796:13365881:1 gene:F32H5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13363795-13365881(+) 100.0 100.0
Public Domain and Genome References
Gene Title transport protein
Ensembl F32H5.4
Entrez Gene 185216 Entrez gene
SwissProt P91993 EMBL-EBI
WormBase CE09888 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04250 Hypothetical protein F32H5.4 [Caenorhabditis elegans] ref|NP_506313.1| F32H5.4 [Caenorhabditis elegans] 0.0
blast CAE70753 Hypothetical protein CBG17501 [Caenorhabditis briggsae] 0.0
blast CAE71545 Hypothetical protein CBG18488 [Caenorhabditis briggsae] 6.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR005828 EMBL-EBI General substrate transporter 5.4E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506313.1 9 37-59,149-167,171-193,205-227,231-250,315-337,352-374,387-409,414-433
F32H5.4 9 37-59,149-167,171-193,205-227,231-250,315-337,352-374,387-409,414-433
SNAP00000043410 7 37-59,149-167,171-193,205-227,231-250,315-337,352-374
GENEFINDER00000043415 9 37-59,149-167,171-193,205-227,231-250,315-337,352-374,387-409,414-433
Sequence
Target Sequence 
>C. ELEGANS:189459_AT
acatggcctcagtgacgatgattctatggctgggaatgttttccacctctttcacaaact
atggttttgtgttcaatatagaaaagttatctggctccctttatatcaatgcattgctca
tgggctcattaagatgggtgctgaatatctttttcggattagctgatttgaaatttgcaa
aattgggtagaaaacacatccatttgatttcaaagttgacaattactatttgtgtgtttt
ctatttttgttacatattactttgaatacgaagaagattactcattaatcatccgtgtag
caactctacttgcttctgccacagcttctcaagttttcatcacaaaatctatggtgctca
tggaattttatccaacggtgattcgtaattctgcggtatcatttaaatcaagtgcaagta
gaattgggacaattctaggaccacaacttttcattttgtgcccatacaaaagtcttccat
atgcaattctgacgggattttgcttgtttgatgcaattgcattccagctgagacttccag
aaactaaaggaaagccactgccagaaacaatgcctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACATGGCCTCAGTGACGATGATTCT 457 107 971 Antisense
ATGGCTGGGAATGTTTTCCACCTCT 412 53 996 Antisense
TTTCCACCTCTTTCACAAACTATGG 305 669 1010 Antisense
GGCTCCCTTTATATCAATGCATTGC 590 539 1063 Antisense
TGTAGCAACTCTACTTGCTTCTGCC 167 561 1266 Antisense
ACGGTGATTCGTAATTCTGCGGTAT 603 95 1345 Antisense
GACAATTCTAGGACCACAACTTTTC 466 363 1398 Antisense
CAACTTTTCATTTTGTGCCCATACA 111 189 1414 Antisense
TTCTGACGGGATTTTGCTTGTTTGA 64 693 1457 Antisense
GCAATTGCATTCCAGCTGAGACTTC 196 323 1483 Antisense
AAGCCACTGCCAGAAACAATGCCTG 301 149 1522 Antisense
Sequence Source Affymetrix Proprietary Database