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[189455_at] acid phosphotase

Description C27A2.4 /REP_DB=WormBase Gene ID /WP=CE04104 /TR=Q18236 /GB=AAK31462.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=acid phosphotase
Cluster 6grp_high1_10_10 > 52      4grp_high_8_8 > 54      6grp_total > 62      4grp_total > 57      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 253
1 2 3 4 range
A 148.1
(-0.3)
P 		240.8
(3)
P 		229.4
(2.6)
P 		69.8
(-3)
A 		171
B 140.8
(-1.4)
P 		265.7
(3)
P 		265.2
(3)
P 		94.4
(-3)
P 		171
C 138.9
(-2.4)
P 		208
(-0.3)
P 		322.6
(3)
P 		116.8
(-3)
A 		206
avr 142.6
(-1.4) 		238.2
(1.9) 		272.4
(3) 		93.7
(-3) 		179

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 191
1 2 3 4 5 6 range
A 100.7
(-2.4)
A 		172.1
(0.2)
P 		245.8
(3)
P 		185
(0.7)
P 		129.3
(-1.4)
P 		85.4
(-3)
A 		160
B 73
(-3)
A 		161.3
(2.6)
P 		167
(3)
P 		166.2
(2.9)
P 		134.6
(0.9)
P 		111.6
(-0.5)
P 		94
C 54.9
(-3)
P 		174.3
(1.3)
P 		191.5
(1.9)
P 		221.3
(3)
P 		134.2
(-0.1)
P 		81.3
(-2)
P 		166
avr 76.2
(-3) 		169.2
(1.5) 		201.4
(3) 		190.8
(2.5) 		132.7
(-0.3) 		92.8
(-2.2) 		125
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 53.9
(2.3)
P 		52.3
(2.1)
P 		60
(3)
P 		10.2
(-3)
A 		50
B 44.9
(0.3)
P 		65.6
(3)
P 		61.5
(2.5)
P 		20.1
(-3)
P 		46
C 46
(-1.3)
P 		59.8
(-0.4)
P 		112.4
(3)
P 		19.7
(-3)
A 		93
avr 48.3
(0.1) 		59.2
(1.2) 		78
(3) 		16.7
(-3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 18.6
(-3)
A 		61.2
(3)
P 		53.5
(1.9)
P 		46.9
(1)
P 		39
(-0.1)
P 		20.8
(-2.7)
A 		43
B 25.4
(-3)
A 		53.7
(2.8)
P 		40.6
(0.1)
P 		44
(0.8)
P 		54.7
(3)
P 		41.6
(0.3)
P 		29
C 20.3
(-3)
P 		53.2
(3)
P 		48.7
(2.2)
P 		44.5
(1.4)
P 		43.4
(1.2)
P 		27.2
(-1.7)
P 		33
avr 21.4
(-3) 		56
(3) 		47.6
(1.5) 		45.1
(1.1) 		45.7
(1.2) 		29.9
(-1.5) 		35
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189455_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4818
Sequence Type Exemplar sequence
Representative Public ID C27A2.4 NCBI
Target Description C27A2.4 /REP_DB=WormBase Gene ID /WP=CE04104 /TR=Q18236 /GB=AAK31462.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=acid phosphotase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062533(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062533 NCBI Caenorhabditis elegans C27A2.4 (C27A2.4) mRNA, complete cds. 11/11 None
SNAP00000044047 ENSEMBL cdna:SNAP chromosome:CEL140:II:5054245:5058097:-1 10/11 None
GENEFINDER00000044064 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5056212:5060696:-1 10/11 None
C27A2.4 ENSEMBL cdna:known chromosome:CEL140:II:5056212:5059941:-1 gene:C27A2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5056209-5059939(-) 100.0 100.0
Public Domain and Genome References
Gene Title acid phosphotase
Ensembl C27A2.4
Entrez Gene 182948 Entrez gene
SwissProt Q18236 EMBL-EBI
WormBase CE04104 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3993 acid phosphatase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494934 C27A2.4 [Caenorhabditis elegans] gb|AAK31462.1| Hypothetical protein C27A2.4 [Caenorhabditis elegans] 0.0
blast CAE59030 Hypothetical protein CBG02310 [Caenorhabditis briggsae] 0.0
blast NP_494934 C27A2.4 [Caenorhabditis elegans] gb|AAK31462.1| Hypothetical protein C27A2.4 [Caenorhabditis elegans] 1.0E-124
blast NP_494931 Interactor of FizzY protein family member (ify-1) [Caenorhabditis elegans] gb|AAK31461.1| Interactor of fizzy protein protein 1 [Caenorhabditis elegans] 1.0E-117
blast NP_494934 C27A2.4 [Caenorhabditis elegans] gb|AAK31462.1| Hypothetical protein C27A2.4 [Caenorhabditis elegans] 1.0E-104
blast CAA92657 Hypothetical protein F07H5.9 [Caenorhabditis elegans] ref|NP_495875.2| F07H5.9 [Caenorhabditis elegans] 1.0E-78
blast CAE59030 Hypothetical protein CBG02310 [Caenorhabditis briggsae] 3.0E-77
blast CAA92657 Hypothetical protein F07H5.9 [Caenorhabditis elegans] ref|NP_495875.2| F07H5.9 [Caenorhabditis elegans] 7.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 2.0E-24
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 1.0E-5
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 4.9E-13
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 4.6E-5
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 4.0E-11
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 1.0E-5
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 4.6E-5
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 3.8E-11
Sequence
Target Sequence 
>C. ELEGANS:189455_AT
gaccttcaaaagcattcgttacgggactttatgatccgccaattgttcaaggcatcaatc
atgatcacacagtattcgctctcctcgctgtgttaggaattgaggatattgttgcaggac
ctgagaaatatggcgagtggcctgactatgcttcagatatcgcgatcgaactgttccata
ataaaacggacgagaagccctatttcagagtgctgtatcagaaaaatgttcattcaactt
tcgaaactgtcacgtcgagaatcaagggatgccgaggagaacagttctgcgacatgcgaa
cattcgaaaacaaagcaaaggaatcccggccagaccgacccatccacgagttctgcgaaa
ta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACCTTCAAAAGCATTCGTTACGGG 432 381 1094 Antisense
GTTACGGGACTTTATGATCCGCCAA 348 445 1111 Antisense
TCATGATCACACAGTATTCGCTCTC 202 623 1152 Antisense
CGCTCTCCTCGCTGTGTTAGGAATT 541 255 1170 Antisense
GAGTGGCCTGACTATGCTTCAGATA 622 401 1228 Antisense
GATATCGCGATCGAACTGTTCCATA 55 427 1249 Antisense
GAAGCCCTATTTCAGAGTGCTGTAT 450 343 1287 Antisense
ATTCAACTTTCGAAACTGTCACGTC 7 13 1325 Antisense
GGAGAACAGTTCTGCGACATGCGAA 107 519 1369 Antisense
CAAAGCAAAGGAATCCCGGCCAGAC 77 191 1404 Antisense
ACCCATCCACGAGTTCTGCGAAATA 250 91 1431 Antisense
Sequence Source Affymetrix Proprietary Database