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[189450_at] Aminopeptidase

Description B0285.7 /REP_DB=WormBase Gene ID /WP=CE00646 /TR=SW:P46557 /GB=CAA84298.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Aminopeptidase
Cluster 6grp_total > 36      4grp_total > 46      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 167.9
(3)
P 		120.7
(1.1)
A 		78.8
(-0.7)
A 		22
(-3)
A 		146
B 181.9
(3)
P 		35.1
(-3)
A 		66.4
(-1.7)
M 		53.4
(-2.3)
A 		147
C 131.4
(3)
P 		83.9
(-0.2)
A 		41
(-3)
P 		77.2
(-0.6)
A 		90
avr 160.4
(3) 		79.9
(-1.4) 		62.1
(-2.4) 		50.9
(-3) 		110

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 222
1 2 3 4 5 6 range
A 109.3
(0.6)
P 		148.5
(3)
P 		72.5
(-1.6)
A 		94.3
(-0.3)
M 		48.8
(-3)
A 		121.3
(1.4)
P 		100
B 110.3
(2.2)
P 		121
(3)
P 		76.6
(-0.3)
M 		50.1
(-2.2)
A 		39.7
(-3)
A 		96.1
(1.2)
A 		81
C 261.5
(3)
P 		107.8
(-1.4)
P 		94.3
(-1.8)
A 		79.3
(-2.2)
A 		50.8
(-3)
A 		96
(-1.7)
P 		211
avr 160.4
(3) 		125.8
(1.2) 		81.1
(-1.2) 		74.6
(-1.5) 		46.4
(-3) 		104.5
(0.1) 		114
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 61.1
(3)
P 		26.2
(-0.6)
A 		20.6
(-1.2)
A 		3.2
(-3)
A 		58
B 58
(3)
P 		8.7
(-3)
A 		15.4
(-2.2)
M 		11.4
(-2.7)
A 		49
C 43.5
(3)
P 		24.1
(-0.8)
A 		14.3
(-2.7)
P 		13
(-3)
A 		31
avr 54.2
(3) 		19.7
(-1.6) 		16.8
(-2) 		9.2
(-3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 20.1
(-2.1)
P 		52.8
(3)
P 		15.8
(-2.8)
A 		23.9
(-1.6)
M 		14.7
(-3)
A 		29.5
(-0.7)
P 		38
B 38.4
(2.6)
P 		40.3
(3)
P 		18.6
(-1.8)
M 		13.3
(-3)
A 		16.1
(-2.4)
A 		35.8
(2)
A 		27
C 96.9
(3)
P 		32.9
(-1.7)
P 		24
(-2.4)
A 		16
(-3)
A 		16.4
(-3)
A 		32.1
(-1.8)
P 		81
avr 51.8
(3) 		42
(1.4) 		19.5
(-2.4) 		17.7
(-2.7) 		15.7
(-3) 		32.5
(-0.2) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189450_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7679
Sequence Type Exemplar sequence
Representative Public ID B0285.7 NCBI
Target Description B0285.7 /REP_DB=WormBase Gene ID /WP=CE00646 /TR=SW:P46557 /GB=CAA84298.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Aminopeptidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065477(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065477 NCBI Caenorhabditis elegans B0285.7 (Aminopeptidase) mRNA, complete cds. 11/11 None
GENEFINDER00000026100 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4356653:4364562:1 11/11 None
SNAP00000026090 ENSEMBL cdna:SNAP chromosome:CEL140:III:4363068:4364562:1 11/11 None
B0285.7 ENSEMBL cdna:known chromosome:CEL140:III:4356508:4364941:1 gene:B0285.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4356652-4364562(+) 100.0 100.0
Public Domain and Genome References
Gene Title Aminopeptidase
Ensembl B0285.7
Entrez Gene 175564 Entrez gene
SwissProt P46557 EMBL-EBI
WormBase CE00646 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4179 membrane alanyl aminopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA84298 Hypothetical protein B0285.7 [Caenorhabditis elegans] ref|NP_497878.1| B0285.7 [Caenorhabditis elegans] sp|P46557|YKG7_CAEEL Hypothetical protein B0285.7 in chromosome III precursor 0.0
blast CAE71220 Hypothetical protein CBG18084 [Caenorhabditis briggsae] 0.0
blast CAA84298 Hypothetical protein B0285.7 [Caenorhabditis elegans] ref|NP_497878.1| B0285.7 [Caenorhabditis elegans] sp|P46557|YKG7_CAEEL Hypothetical protein B0285.7 in chromosome III precursor 1.0E-167
blast CAE71220 Hypothetical protein CBG18084 [Caenorhabditis briggsae] 1.0E-155
blast AAF55912 CG31233-PA [Drosophila melanogaster] gb|AAM50162.1| GH12469p [Drosophila melanogaster] ref|NP_732653.2| CG31233-PA [Drosophila melanogaster] 6.0E-9
Protein Domains
Database ID Description E-Value
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 3.0E-110
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 2.5E-82
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 8.6E-19
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 4.4E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497878.1 1 16-38
B0285.7 1 16-38
GENEFINDER00000026100 1 16-38
Sequence
Target Sequence 
>C. ELEGANS:189450_AT
agccaccatctctgattgggcatcagcagccgaaacagcaaccgggggacgcccagaagc
cggagctctcattcatcaatggtaccgcccgtcttccacaatttctcgcccagtccttcg
tgcagtcgtctcatcgaatagtgttgaattcaatcaattgacagaggaaacgtggacggt
cccgctggaaatttctggatctgctggaactcagttggctgtaatttcagagaagaaaca
agccattccatttgtatccagtgattatgttgttgtggatgctggtaggaaatcacacgc
ttttgttgtatacgatgcagatacataccttcgattaataagatgctttggagactctag
atgtccatcaaaagaaattggaggaattttctccgatctgggagccgctcttcttgcaaa
tatattaccgaaaccagaaaatcaagatgttgcaaaatggaaatctgtgtttaagttcat
ggctcaacaaaatatcgtggaaggaactgcggcttgttgtgtggagcacgcaatccgaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCCACCATCTCTGATTGGGCATCA 16 85 1737 Antisense
GCTCTCATTCATCAATGGTACCGCC 521 299 1801 Antisense
CTTCGTGCAGTCGTCTCATCGAATA 12 221 1852 Antisense
CAGAGGAAACGTGGACGGTCCCGCT 155 205 1898 Antisense
CGGTCCCGCTGGAAATTTCTGGATC 69 245 1913 Antisense
GGATCTGCTGGAACTCAGTTGGCTG 450 511 1933 Antisense
AAGCCATTCCATTTGTATCCAGTGA 497 147 1976 Antisense
AATTTTCTCCGATCTGGGAGCCGCT 345 167 2121 Antisense
GCCGCTCTTCTTGCAAATATATTAC 670 285 2140 Antisense
GAAGGAACTGCGGCTTGTTGTGTGG 540 333 2236 Antisense
GTTGTGTGGAGCACGCAATCCGAGA 334 437 2252 Antisense
Sequence Source Affymetrix Proprietary Database