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[189444_at] cytochrome p450

Description ZK177.5 /REP_DB=WormBase Gene ID /WP=CE25682 /GEN=cyp-44 /GB=AAG00050.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=cytochrome p450
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 95      4grp_highest_8_8 > 29      4grp_high_8_8 > 07      6grp_highest_nonorm > 82      6grp_total > 76      4grp_total > 06      6grp_cgc > 67      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 272
1 2 3 4 range
A 334.8
(2.8)
P 		183
(-3)
P 		339.8
(3)
P 		308.2
(1.8)
P 		157
B 326
(-0.9)
P 		323.1
(-1.3)
P 		308.9
(-3)
P 		357.7
(3)
P 		49
C 305.6
(-2.2)
P 		300.8
(-2.4)
P 		283.7
(-3)
P 		455.2
(3)
P 		172
avr 322.1
(0) 		269
(-3) 		310.8
(-0.6) 		373.7
(3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 399
1 2 3 4 5 6 range
A 233.6
(-3)
P 		287.4
(-1.7)
P 		357
(-0.1)
P 		406.9
(1.1)
P 		364.9
(0.1)
P 		486.2
(3)
P 		253
B 299.1
(-3)
P 		301.3
(-2.9)
P 		333.3
(-2.1)
P 		389.7
(-0.6)
P 		380.9
(-0.8)
P 		522.4
(3)
P 		223
C 199.3
(-3)
P 		325.3
(-1.1)
P 		343.9
(-0.8)
P 		443.7
(0.7)
P 		331.2
(-1)
P 		598.6
(3)
P 		399
avr 244
(-3) 		304.7
(-1.8) 		344.7
(-0.9) 		413.4
(0.5) 		359
(-0.6) 		535.7
(3) 		292
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 121.9
(3)
P 		39.7
(-3)
P 		88.8
(0.6)
P 		45.1
(-2.6)
P 		82
B 104
(3)
P 		79.8
(-1.5)
P 		71.6
(-3)
P 		76.2
(-2.1)
P 		32
C 101.2
(3)
P 		86.5
(-0.6)
P 		98.9
(2.4)
P 		76.8
(-3)
P 		24
avr 109
(3) 		68.7
(-2.6) 		86.4
(-0.2) 		66
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 43.1
(-3)
P 		102.2
(1.7)
P 		77.7
(-0.2)
P 		103.2
(1.8)
P 		110.1
(2.3)
P 		118.4
(3)
P 		75
B 104.1
(-1.8)
P 		100.3
(-2)
P 		80.9
(-3)
P 		103.2
(-1.8)
P 		154.7
(0.9)
P 		194.8
(3)
P 		114
C 73.9
(-3)
P 		99.3
(-1.8)
P 		87.5
(-2.4)
P 		89.2
(-2.3)
P 		107.2
(-1.4)
P 		200.4
(3)
P 		127
avr 73.7
(-3) 		100.6
(-1.3) 		82
(-2.5) 		98.5
(-1.5) 		124
(0.1) 		171.2
(3) 		98
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189444_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5836
Sequence Type Exemplar sequence
Representative Public ID ZK177.5 NCBI
Target Description ZK177.5 /REP_DB=WormBase Gene ID /WP=CE25682 /GEN=cyp-44 /GB=AAG00050.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=cytochrome p450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062651(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062651 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-44A1) (cyp-44A1) mRNA, complete cds. 11/11 None
SNAP00000012884 ENSEMBL cdna:SNAP chromosome:CEL140:II:5504017:5508945:1 11/11 None
GENEFINDER00000012901 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5504017:5505867:1 11/11 None
ZK177.5.2 ENSEMBL cdna:known chromosome:CEL140:II:5503936:5506085:1 gene:ZK177.5 11/11 None
ZK177.5.1 ENSEMBL cdna:known chromosome:CEL140:II:5504014:5506085:1 gene:ZK177.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5504014-5505865(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome p450
Gene Symbol cyp-44A1
Ensembl ZK177.5
Entrez Gene 173936 Entrez gene
SwissProt Q09660 EMBL-EBI
WormBase CE25682 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.18414.1.S1_AT similar to Cytochrome P450 24A1, mitochondrial precursor (P450-CC24) (Vitamin D(3) 24-hydroxylase) (1,25-dihydroxyvitamin D(3) 24-hydroxylase) (24-OHase) cfa
DROSOPHILA_2:1630170_AT dm
DROSGENOME1:147464_AT dm
CHICKEN:GGA.893.1.S1_A_AT cytochrome P450, family 24, subfamily A, polypeptide 1 gga
HG-U95AV2:660_AT cytochrome P450, family 24, subfamily A, polypeptide 1 hs
HC-G110:660_AT cytochrome P450, family 24, subfamily A, polypeptide 1 hs
HU35KSUBA:U60669_RNA1_S_AT cytochrome P450, family 24, subfamily A, polypeptide 1 hs
HUGENEFL:L13286_AT cytochrome P450, family 24, subfamily A, polypeptide 1 hs
HG-U133A:206504_AT cytochrome P450, family 24, subfamily A, polypeptide 1 hs
HG-U133_PLUS_2:206504_AT cytochrome P450, family 24, subfamily A, polypeptide 1 hs
HG-FOCUS:206504_AT cytochrome P450, family 24, subfamily A, polypeptide 1 hs
HG-U133A_2:206504_AT cytochrome P450, family 24, subfamily A, polypeptide 1 hs
U133_X3P:G4503204_3P_AT cytochrome P450, family 24, subfamily A, polypeptide 1 hs
HG-U95E:71346_AT cytochrome P450, family 24, subfamily A, polypeptide 1 hs
MU11KSUBA:D89669_AT cytochrome P450, family 24, subfamily a, polypeptide 1 mm
MU11KSUBB:MSA.1752.0_S_AT cytochrome P450, family 24, subfamily a, polypeptide 1 mm
MG-U74AV2:93435_AT cytochrome P450, family 24, subfamily a, polypeptide 1 mm
MOE430A:1418866_AT cytochrome P450, family 24, subfamily a, polypeptide 1 mm
MOUSE430A_2:1418866_AT cytochrome P450, family 24, subfamily a, polypeptide 1 mm
MOUSE430_2:1418866_AT cytochrome P450, family 24, subfamily a, polypeptide 1 mm
MOE430A:1418867_AT cytochrome P450, family 24, subfamily a, polypeptide 1 mm
MOUSE430A_2:1418867_AT cytochrome P450, family 24, subfamily a, polypeptide 1 mm
MOUSE430_2:1418867_AT cytochrome P450, family 24, subfamily a, polypeptide 1 mm
MG-U74AV2:162070_R_AT Cytochrome P450, family 24, subfamily a, polypeptide 1 (Cyp24a1), mRNA mm
RT-U34:L04619_S_AT cytochrome P450, subfamily 24 rn
RG-U34A:L04619_S_AT cytochrome P450, subfamily 24 rn
RG-U34A:Z28351EXON_S_AT cytochrome P450, subfamily 24 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAG00050 Cytochrome p450 family protein 44A1 [Caenorhabditis elegans] ref|NP_495052.1| CYtochrome P450 family member (cyp-44A1) [Caenorhabditis elegans] sp|Q09660|CC44_CAEEL Probable cytochrome P450 CYP44 0.0
blast CAE59123 Hypothetical protein CBG02418 [Caenorhabditis briggsae] 0.0
blast T27763 hypothetical protein ZK177.5 - Caenorhabditis elegans 0.0
blast AAG00052 Hypothetical protein ZK177.4 [Caenorhabditis elegans] ref|NP_495053.1| ZK177.4a [Caenorhabditis elegans] sp|Q09372|YS44_CAEEL Hypothetical protein ZK177.4 in chromosome II 0.0
Protein Families
Method ID Description E-Value
p450 Q13120 Animalia; CYP2A6V2; hum; Q13120; Homo sapiens; Q13120 4.0E-27
p450 CP11_ONCMY Animalia; CYP1A2; tro; CP11_ONCMY; Trout; Q92110 5.0E-75
p450 CP11_ONCMY Animalia; CYP1A2; tro; CP11_ONCMY; Trout; Q92110 9.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.0E-43
Pfam IPR001128 EMBL-EBI Cytochrome P450 7.8E-52
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.4E-52
Sequence
Target Sequence 
>C. ELEGANS:189444_AT
cagatggtctgagcacgactgcaccgatgctaatatacaacctatataatctcgcaacac
atcctgaagcactaaaagaaattcaaaaagaaataaaagaagatccagcatcttccaagc
ttacattcctccgtgcttgtatcaaggaaacgttccgaatgtttccaattgggactgaag
tttcaagagtcacccagaaaaatttaatactgtccggatatgaagtacctgctgggacag
ctgttgacatcaatacaaatgttctaatgagacacgaagttctgttctctgattctcccc
gcgaattcaagcctcaacgatggcttgaaaaatcgaaagaagttcatccgtttgcttatc
ttccgttcggattcgggccacggatgtgcgctggtcgacgattcgccgagcaggatctac
taacatcacttgccaagctgtgtggaaattatgatattcgtcacagaggagatccaataa
ctcagatctatgaaactcttcttctaccacgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGATGGTCTGAGCACGACTGCACC 548 205 938 Antisense
TAATCTCGCAACACATCCTGAAGCA 391 635 984 Antisense
GATCCAGCATCTTCCAAGCTTACAT 42 417 1039 Antisense
CAAGCTTACATTCCTCCGTGCTTGT 94 185 1053 Antisense
CACGAAGTTCTGTTCTCTGATTCTC 623 199 1210 Antisense
CCGCGAATTCAAGCCTCAACGATGG 281 263 1236 Antisense
AGAAGTTCATCCGTTTGCTTATCTT 318 75 1275 Antisense
GCTTATCTTCCGTTCGGATTCGGGC 430 305 1291 Antisense
GACGATTCGCCGAGCAGGATCTACT 638 377 1334 Antisense
ATCTACTAACATCACTTGCCAAGCT 91 33 1352 Antisense
CTATGAAACTCTTCTTCTACCACGT 548 217 1425 Antisense
Sequence Source Affymetrix Proprietary Database