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[189439_at]

Description K10F12.3 /REP_DB=WormBase Gene ID /WP=CE12090 /TR=O17232 /GB=AAB71005.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=phospholipase C
Cluster 6grp_total > 06      4grp_total > 47      6grp_cgc > 22      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 240
1 2 3 4 range
A 264.3
(3)
A 		118.4
(-3)
A 		131.4
(-2.5)
A 		152.3
(-1.6)
A 		146
B 304.5
(3)
A 		133.5
(-1.3)
A 		64.8
(-3)
A 		154.8
(-0.7)
A 		240
C 275.1
(3)
A 		129.6
(-2.1)
A 		125.7
(-2.3)
A 		104.5
(-3)
A 		171
avr 281.3
(3) 		127.2
(-2.3) 		107.3
(-3) 		137.2
(-2) 		174

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 220
1 2 3 4 5 6 range
A 166.4
(3)
A 		149
(2.3)
A 		92.3
(-0.2)
A 		78.2
(-0.8)
A 		69.8
(-1.1)
A 		25.5
(-3)
A 		141
B 164.5
(3)
A 		130.5
(1.3)
A 		42.5
(-3)
A 		91
(-0.6)
A 		75.8
(-1.4)
A 		49.5
(-2.7)
A 		122
C 234.4
(3)
A 		98.5
(-0.7)
A 		25.2
(-2.7)
A 		14.9
(-3)
A 		98.7
(-0.7)
A 		15.5
(-3)
A 		220
avr 188.4
(3) 		126
(0.6) 		53.3
(-2.1) 		61.4
(-1.8) 		81.4
(-1.1) 		30.2
(-3) 		158
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 96.2
(3)
A 		25.7
(-2.7)
A 		34.4
(-2)
A 		22.3
(-3)
A 		74
B 97.1
(3)
A 		33
(-1.7)
A 		15
(-3)
A 		33
(-1.7)
A 		82
C 91.1
(3)
A 		37.3
(-1.4)
A 		43.8
(-0.9)
A 		17.6
(-3)
A 		74
avr 94.8
(3) 		32
(-2.3) 		31.1
(-2.4) 		24.3
(-3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 30.7
(0.1)
A 		53
(3)
A 		20.1
(-1.2)
A 		19.8
(-1.3)
A 		21.1
(-1.1)
A 		6.2
(-3)
A 		47
B 57.2
(3)
A 		43.4
(1.2)
A 		10.3
(-3)
A 		24.1
(-1.2)
A 		30.8
(-0.4)
A 		18.4
(-2)
A 		47
C 86.9
(3)
A 		30.1
(-1.1)
A 		6.4
(-2.8)
A 		3
(-3)
A 		31.9
(-0.9)
A 		5.2
(-2.8)
A 		84
avr 58.3
(3) 		42.2
(1) 		12.3
(-2.7) 		15.6
(-2.3) 		27.9
(-0.8) 		9.9
(-3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189439_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8310
Sequence Type Exemplar sequence
Representative Public ID K10F12.3 NCBI
Target Description K10F12.3 /REP_DB=WormBase Gene ID /WP=CE12090 /TR=O17232 /GB=AAB71005.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=phospholipase C
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171059(11), NM_171060(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171059 NCBI Caenorhabditis elegans PhosphoLipase C Like family member (pll-1) (pll-1) mRNA, complete cds. 11/11 A 
NM_171060 NCBI Caenorhabditis elegans PhosphoLipase C Like family member (pll-1) (pll-1) mRNA, complete cds. 11/11 A 
GENEFINDER00000031886 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:365953:374228:1 11/11 None
SNAP00000031876 ENSEMBL cdna:SNAP chromosome:CEL140:III:370385:374228:1 11/11 None
K10F12.3b ENSEMBL cdna:known chromosome:CEL140:III:365948:377550:1 gene:K10F12.3 11/11 A 
K10F12.3a ENSEMBL cdna:known chromosome:CEL140:III:367325:377457:1 gene:K10F12.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:368516-373733(+) 100.0 100.0
Public Domain and Genome References
Ensembl K10F12.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN72423 Phospholipase c like protein 1, isoform b [Caenorhabditis elegans] ref|NP_741067.1| PhosphoLipase C Like family member (pll-1) [Caenorhabditis elegans] 0.0
blast AAN72422 Phospholipase c like protein 1, isoform a [Caenorhabditis elegans] ref|NP_741068.1| PhosphoLipase C Like family member (pll-1) [Caenorhabditis elegans] 0.0
blast T32374 hypothetical protein K10F12.3 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 1.1E-23
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 2.1E-33
Pfam IPR000008 EMBL-EBI C2 domain 5.5E-27
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 1.1E-23
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 2.1E-33
Pfam IPR000008 EMBL-EBI C2 domain 5.5E-27
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 1.1E-23
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 2.1E-33
Pfam IPR000008 EMBL-EBI C2 domain 5.5E-27
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741068.1 1 13-30
K10F12.3a 1 13-30
Sequence
Target Sequence 
>C. ELEGANS:189439_AT
ttcggattgcccggagactgtgccgaggagcgcacaagaacagttaggaatgatagcatc
aacccatccttcgacgagtccttccaattccaagtctccgtacccgagctcgcgttggtc
cgattccttgtcctcgacgatgactacatcggagacgacttcatcggtcaatacacaatt
ccattcgaatgcctacaacctggatatcggcatatctatctgctgaataatgaaggtgac
ccgttggagcatgccacgttgtttgtccacgtggcgattacgaatcgacgtggcggtggt
aaggcgaagaagcgtggaatgtcggtgaagcggaagaactcgaggatctcgtcggggatg
aaactggttgggattaaaagtgtcgatgatcagtttaaggtcgccgttgtccctctcgcc
gagtcaacagcgatccgcaaccgtctcgagaacgcaatgatcgactggcaggaggagtgc
ggtctgggacccgctgggacaattcgtcaaggcattcgactcatccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGGATTGCCCGGAGACTGTGCCG 466 695 2089 Antisense
GAATGATAGCATCAACCCATCCTTC 366 331 2136 Antisense
TTCCTTGTCCTCGACGATGACTACA 251 701 2212 Antisense
ACATCGGAGACGACTTCATCGGTCA 514 105 2234 Antisense
TGAAGGTGACCCGTTGGAGCATGCC 528 575 2319 Antisense
GCATGCCACGTTGTTTGTCCACGTG 171 311 2337 Antisense
GATCAGTTTAAGGTCGCCGTTGTCC 699 417 2476 Antisense
TCGCCGAGTCAACAGCGATCCGCAA 498 597 2504 Antisense
GGCAGGAGGAGTGCGGTCTGGGACC 510 525 2555 Antisense
ACCCGCTGGGACAATTCGTCAAGGC 126 39 2577 Antisense
TCGTCAAGGCATTCGACTCATCCAC 325 605 2592 Antisense
Sequence Source Affymetrix Proprietary Database