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[189430_at] cytochrome P450

Description C12D5.7 /REP_DB=WormBase Gene ID /WP=CE06809 /TR=Q27470 /GB=AAA98571.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 52      4grp_highest_8_8 > 19      4grp_high_8_8 > 63      6grp_highest_nonorm > 53      6grp_total > 30      4grp_total > 40      6grp_highest_sub > 03      6grp_cgc > 83      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 434
1 2 3 4 range
A 462.2
(-3)
P 		634.9
(-0.2)
P 		838.3
(3)
P 		644.7
(-0.1)
P 		376
B 553.6
(-2.6)
P 		532.3
(-3)
P 		895.9
(3)
P 		593.9
(-2)
P 		364
C 507.1
(-3)
P 		551.5
(-2.2)
P 		852.9
(3)
P 		556.6
(-2.1)
P 		346
avr 507.6
(-3) 		572.9
(-1.9) 		862.4
(3) 		598.4
(-1.5) 		355

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 557
1 2 3 4 5 6 range
A 639.9
(-2.6)
P 		1029.8
(2.6)
P 		1063.1
(3)
P 		881.9
(0.6)
P 		708.9
(-1.7)
P 		608.8
(-3)
P 		454
B 553.3
(-3)
P 		883.9
(1.2)
P 		931.1
(1.8)
P 		1021.3
(3)
P 		699.7
(-1.1)
P 		628.8
(-2)
P 		468
C 506.6
(-3)
P 		803.3
(0.2)
P 		1055.6
(3)
P 		980.5
(2.2)
P 		509
(-3)
P 		636.7
(-1.6)
P 		549
avr 566.6
(-3) 		905.7
(1.5) 		1016.6
(3) 		961.2
(2.3) 		639.2
(-2) 		624.8
(-2.2) 		450
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 204
1 2 3 4 range
A 168.3
(0.6)
P 		137.8
(-0.9)
P 		219.1
(3)
P 		94.4
(-3)
P 		125
B 176.5
(0.7)
P 		131.5
(-2.6)
P 		207.6
(3)
P 		126.6
(-3)
P 		81
C 167.9
(-0.8)
P 		158.6
(-1.1)
P 		297.3
(3)
P 		93.8
(-3)
P 		204
avr 170.9
(-0.1) 		142.6
(-1.3) 		241.3
(3) 		104.9
(-3) 		136

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 248
1 2 3 4 5 6 range
A 118
(-3)
P 		366.2
(3)
P 		231.5
(-0.3)
P 		223.7
(-0.4)
P 		213.9
(-0.7)
P 		148.2
(-2.3)
P 		248
B 192.5
(-3)
P 		294.2
(3)
P 		226.1
(-1)
P 		270.5
(1.6)
P 		284.3
(2.4)
P 		234.5
(-0.5)
P 		102
C 187.8
(-1.7)
P 		245.1
(1.6)
P 		268.7
(3)
P 		197.2
(-1.1)
P 		164.7
(-3)
P 		213.1
(-0.2)
P 		104
avr 166.1
(-3) 		301.8
(3) 		242.1
(0.4) 		230.5
(-0.2) 		221
(-0.6) 		198.6
(-1.6) 		136
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189430_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11921
Sequence Type Exemplar sequence
Representative Public ID C12D5.7 NCBI
Target Description C12D5.7 /REP_DB=WormBase Gene ID /WP=CE06809 /TR=Q27470 /GB=AAA98571.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072587(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072587 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-33A1) (cyp-33A1) mRNA, complete cds. 11/11 None
SNAP00000021441 ENSEMBL cdna:SNAP chromosome:CEL140:V:7681992:7684025:-1 11/11 None
GENEFINDER00000021454 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7681992:7684025:-1 11/11 None
C12D5.7 ENSEMBL cdna:known chromosome:CEL140:V:7681929:7684025:-1 gene:C12D5.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7681992-7684026(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-33A1
Ensembl C12D5.7
Entrez Gene 179148 Entrez gene
SwissProt Q27470 EMBL-EBI
WormBase CE06809 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250558_AT flavonoid 3'-monooxygenase / flavonoid 3'-hydroxylase (F3'H) / cytochrome P450 75B1 (CYP75B1) / transparent testa 7 protein (TT7) at
CHICKEN:GGA.13084.2.S1_A_AT similar to cytochrome P450 2D20 gga
MOUSE430_2:1425365_A_AT cytochrome P450, family 2, subfamily d, polypeptide 13 mm
MOUSE430A_2:1425365_A_AT cytochrome P450, family 2, subfamily d, polypeptide 13 mm
MOE430A:1425365_A_AT cytochrome P450, family 2, subfamily d, polypeptide 13 mm
MOUSE430A_2:1431803_AT cytochrome P450, family 2, subfamily d, polypeptide 13 mm
MOE430A:1431803_AT cytochrome P450, family 2, subfamily d, polypeptide 13 mm
MOUSE430_2:1431803_AT cytochrome P450, family 2, subfamily d, polypeptide 13 mm
RT-U34:AB008424_S_AT cytochrome P450, family 2, subfamily d, polypeptide 13 rn
RG-U34A:AB008424_S_AT cytochrome P450, family 2, subfamily d, polypeptide 13 rn
RAE230A:1370496_AT cytochrome P450, family 2, subfamily d, polypeptide 13 rn
RAT230_2:1370496_AT cytochrome P450, family 2, subfamily d, polypeptide 13 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA98571 Cytochrome p450 family protein 33A1 [Caenorhabditis elegans] ref|NP_504988.1| CYtochrome P450 family member (cyp-33A1) [Caenorhabditis elegans] 0.0
blast CAE70434 Hypothetical protein CBG17010 [Caenorhabditis briggsae] 0.0
blast CAE71568 Hypothetical protein CBG18519 [Caenorhabditis briggsae] 1.0E-126
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-132
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.3E-107
Sequence
Target Sequence 
>C. ELEGANS:189430_AT
gtgtttcgatatgtgggttgccgggatgcacacaactacaaatacaatgggattcctcac
tgcttttgcacttaataatatggatgctcagaggaaaatgcagaaggaattgacagaagt
aattggagaccgagtggttacaatgaaggataaattgaacttaccatacacaaatgcatt
tattaatgaagctcaacgatgtgccaatctagtcccaatgaatctcccacatgctgttac
tcgtgatgttcaactccttgggtgtacaattccaaagggaacaacagtcattcaccaaat
cagcagtgttatgtcagacccagaaatattcgaagaacccgaaagatttgtgcctgaaag
atatctggatgagagtggaaatctgaaaaaaatcgaagaacttgtcccattctcaattgg
gaaacgggtatgtcttggagaaggacttgctcgaatggaattgttcctgttcactgcaaa
ttctttcaatcgatacgagtttaactgtggatccaatggagttccatcactggagagaac
ttttgctttcatcgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTTTCGATATGTGGGTTGCCGGG 526 485 888 Antisense
TGCCGGGATGCACACAACTACAAAT 103 585 906 Antisense
ATTCCTCACTGCTTTTGCACTTAAT 250 9 939 Antisense
GAAGCTCAACGATGTGCCAATCTAG 81 343 1075 Antisense
GCCAATCTAGTCCCAATGAATCTCC 420 277 1090 Antisense
TCCCACATGCTGTTACTCGTGATGT 327 599 1112 Antisense
GTGATGTTCAACTCCTTGGGTGTAC 666 435 1130 Antisense
AACAGTCATTCACCAAATCAGCAGT 548 135 1170 Antisense
TCAGCAGTGTTATGTCAGACCCAGA 291 623 1187 Antisense
GGAATTGTTCCTGTTCACTGCAAAT 512 533 1344 Antisense
GGAGAGAACTTTTGCTTTCATCGCA 436 519 1419 Antisense
Sequence Source Affymetrix Proprietary Database