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[189421_at] 7TM chemoreceptor, sri family

Description Y27F2A.4 /REP_DB=WormBase Gene ID /WP=CE26056 /GEN=sri-33 /GB=AAK72090.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 08      4grp_total > 11      6grp_cgc > 0      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 2.9
(-3)
A 		21.1
(-1.6)
A 		2.4
(-3)
A 		81.8
(3)
A 		79
B 20
(1.7)
A 		6.5
(-2.1)
A 		3.1
(-3)
A 		24.9
(3)
A 		22
C 11.6
(-1.8)
A 		1.3
(-2.9)
A 		0.6
(-3)
A 		54.9
(3)
A 		54
avr 11.5
(-1.9) 		9.6
(-2.1) 		2
(-3) 		53.9
(3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 53.3
(3)
A 		3.9
(-3)
A 		9.6
(-2.3)
A 		9.6
(-2.3)
A 		7.6
(-2.6)
A 		11
(-2.1)
A 		49
B 26.2
(3)
M 		1.7
(-2.9)
A 		2.6
(-2.6)
A 		10.8
(-0.7)
A 		4.6
(-2.2)
A 		1.1
(-3)
A 		25
C 1.6
(-2.9)
A 		22.3
(1.3)
A 		6.4
(-2)
A 		30.8
(3)
A 		1.3
(-3)
A 		2.2
(-2.8)
A 		30
avr 27
(3) 		9.3
(-1.7) 		6.2
(-2.5) 		17.1
(0.4) 		4.5
(-3) 		4.8
(-2.9) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 1.1
(-2.7)
A 		4.6
(-0.9)
A 		0.6
(-3)
A 		12
(3)
A 		11
B 6.4
(3)
A 		1.6
(-2.1)
A 		0.7
(-3)
A 		5.3
(1.8)
A 		6
C 3.8
(-0.6)
A 		0.4
(-2.9)
A 		0.2
(-3)
A 		9.2
(3)
A 		9
avr 3.8
(-0.6) 		2.2
(-1.8) 		0.5
(-3) 		8.8
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 9.8
(3)
A 		1.4
(-3)
A 		2.1
(-2.5)
A 		2.4
(-2.3)
A 		2.3
(-2.4)
A 		2.7
(-2.1)
A 		8
B 9.1
(3)
M 		0.6
(-2.9)
A 		0.6
(-2.9)
A 		2.9
(-1.3)
A 		1.9
(-2)
A 		0.4
(-3)
A 		9
C 0.6
(-2.8)
A 		6.8
(3)
A 		1.6
(-1.9)
A 		6.2
(2.4)
A 		0.4
(-3)
A 		0.7
(-2.7)
A 		6
avr 6.5
(3) 		2.9
(-1.2) 		1.4
(-2.9) 		3.8
(-0.1) 		1.5
(-2.8) 		1.3
(-3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189421_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5895
Sequence Type Exemplar sequence
Representative Public ID Y27F2A.4 NCBI
Target Description Y27F2A.4 /REP_DB=WormBase Gene ID /WP=CE26056 /GEN=sri-33 /GB=AAK72090.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062061(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062061 NCBI Caenorhabditis elegans Serpentine Receptor, class I family member (sri-33) (sri-33) mRNA, complete cds. 11/11 A 
GENEFINDER00000006781 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3154553:3156377:-1 10/11 None
Y27F2A.4 ENSEMBL cdna:known chromosome:CEL140:II:3155162:3156844:-1 gene:Y27F2A.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000006762 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3155160-3156843(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, sri family
Gene Symbol sri-33
Ensembl Y27F2A.4
Entrez Gene 191908 Entrez gene
SwissProt Q966C4 EMBL-EBI
WormBase CE26056 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494462 Serpentine Receptor, class I family member (sri-33) [Caenorhabditis elegans] gb|AAK72090.1| Serpentine receptor, class i protein 33 [Caenorhabditis elegans] 1.0E-174
blast NP_494463 Serpentine Receptor, class I family member (sri-34) [Caenorhabditis elegans] gb|AAK72091.1| Serpentine receptor, class i protein 34 [Caenorhabditis elegans] 1.0E-136
blast NP_494462 Serpentine Receptor, class I family member (sri-33) [Caenorhabditis elegans] gb|AAK72090.1| Serpentine receptor, class i protein 33 [Caenorhabditis elegans] 1.0E-129
blast NP_494463 Serpentine Receptor, class I family member (sri-34) [Caenorhabditis elegans] gb|AAK72091.1| Serpentine receptor, class i protein 34 [Caenorhabditis elegans] 1.0E-112
blast AAK71402 Serpentine receptor, class i protein 32 [Caenorhabditis elegans] ref|NP_494461.1| Serpentine Receptor, class I family member (sri-32) [Caenorhabditis elegans] 2.0E-76
blast AAK71402 Serpentine receptor, class i protein 32 [Caenorhabditis elegans] ref|NP_494461.1| Serpentine Receptor, class I family member (sri-32) [Caenorhabditis elegans] 2.0E-55
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.0E-31
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.8E-47
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494462.1 7 10-32,45-64,92-114,134-156,186-208,240-262,277-299
Y27F2A.4 7 10-32,45-64,92-114,134-156,186-208,240-262,277-299
GENEFINDER00000006781 6 13-35,53-75,95-117,147-169,200-222,242-264
Sequence
Target Sequence 
>C. ELEGANS:189421_AT
gttcaatatttggcacgtgtttctagcaggaattggaatagttggaggaatattttgtgc
agtaactggagtttatactacagtggatattttcaaaatgttacaagaggtgcagacaaa
agtctccagttcaagctttcaacgcaatcaatctgcaattcgtagcttggtatctcaaat
gttggcttctctcatgttttttgttcccttgttcttttttgtgatgctaatcatgtctga
catggataatgggcagtttattggagaatttctacaatcaatttgtgcgttgcaatctat
tgtgaacgcggtggtgttaatattcacaacgccctgttatagaagtttcgttctacataa
acccgcgcggtgtcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCAATATTTGGCACGTGTTTCTA 504 445 561 Antisense
GGCACGTGTTTCTAGCAGGAATTGG 256 535 572 Antisense
GGAATATTTTGTGCAGTAACTGGAG 623 531 607 Antisense
GGTGCAGACAAAAGTCTCCAGTTCA 250 501 669 Antisense
GGTATCTCAAATGTTGGCTTCTCTC 343 505 729 Antisense
AAATGTTGGCTTCTCTCATGTTTTT 86 121 737 Antisense
GTTCCCTTGTTCTTTTTTGTGATGC 606 441 763 Antisense
TGTGATGCTAATCATGTCTGACATG 488 555 780 Antisense
ATATTCACAACGCCCTGTTATAGAA 232 19 880 Antisense
CGCCCTGTTATAGAAGTTTCGTTCT 606 255 890 Antisense
TTCTACATAAACCCGCGCGGTGTCG 706 689 911 Antisense
Sequence Source Affymetrix Proprietary Database