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[189418_s_at]

Description F25H5.3B /REP_DB=WormBase Gene ID /WP=CE15899 /TR=O17835 /GB=CAB02983.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Pyruvate kinase
Cluster 6grp_highest_10_10 > 46      6grp_high1_10_10 > 64      4grp_highest_8_8 > 10      4grp_high_8_8 > 55      6grp_highest_nonorm > 36      6grp_total > 54      4grp_total > 56      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1259
1 2 3 4 range
A 3519.3
(0.1)
P 		3285.8
(-2.4)
P 		3785.9
(3)
P 		3225.8
(-3)
P 		560
B 3110.5
(-3)
P 		3448.5
(-1)
P 		4130.5
(3)
P 		3722.8
(0.6)
P 		1020
C 3401.2
(-0.4)
P 		4097.4
(3)
P 		3797.9
(1.5)
P 		2871.6
(-3)
P 		1226
avr 3343.7
(-2.3) 		3610.6
(0.2) 		3904.8
(3) 		3273.4
(-3) 		631

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2247
1 2 3 4 5 6 range
A 3412.5
(-2.3)
P 		4629.1
(1.9)
P 		3340.7
(-2.6)
P 		4004.2
(-0.3)
P 		3218.1
(-3)
P 		4936.2
(3)
P 		1718
B 3558
(-2.5)
P 		3571.9
(-2.4)
P 		5233.9
(3)
P 		3725.7
(-1.9)
P 		3393.5
(-3)
P 		3738.6
(-1.9)
P 		1840
C 4943.7
(1.8)
P 		3365.3
(-2.4)
P 		5400.2
(3)
P 		3153.2
(-3)
P 		4544.1
(0.7)
P 		4229.2
(-0.1)
P 		2247
avr 3971.4
(-1) 		3855.4
(-1.7) 		4658.3
(3) 		3627.7
(-3) 		3718.6
(-2.5) 		4301.3
(0.9) 		1031
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 852
1 2 3 4 range
A 1281.4
(3)
P 		713.3
(-1.2)
P 		989.7
(0.8)
P 		472.2
(-3)
P 		809
B 991.9
(3)
P 		851.7
(-1.2)
P 		957.1
(1.9)
P 		793.3
(-3)
P 		199
C 1125.9
(1.6)
P 		1178
(2)
P 		1323.7
(3)
P 		484.2
(-3)
P 		840
avr 1133.1
(3) 		914.3
(0.6) 		1090.2
(2.5) 		583.2
(-3) 		550

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1203
1 2 3 4 5 6 range
A 629.1
(-3)
P 		1646.3
(3)
P 		727.5
(-2.4)
P 		1015.5
(-0.7)
P 		971.1
(-1)
P 		1201.8
(0.4)
P 		1017
B 1238
(0.7)
P 		1189
(-0)
P 		1271
(1.2)
P 		986.8
(-3)
P 		1378.7
(2.8)
P 		1394.1
(3)
P 		407
C 1832.4
(3)
P 		1026.9
(-1)
P 		1374.4
(0.7)
P 		634.1
(-3)
P 		1470.4
(1.2)
P 		1415.8
(0.9)
P 		1198
avr 1233.2
(1.6) 		1287.4
(2.3) 		1124.3
(0.2) 		878.8
(-3) 		1273.4
(2.2) 		1337.2
(3) 		458
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189418_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.724
Sequence Type Exemplar sequence
Representative Public ID F25H5.3B NCBI
Target Description F25H5.3B /REP_DB=WormBase Gene ID /WP=CE15899 /TR=O17835 /GB=CAB02983.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Pyruvate kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060057(11), NM_001026241(11), NM_001026240(11), NM_060058(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060057 NCBI Caenorhabditis elegans F25H5.3a (F25H5.3) mRNA, complete cds. 11/11 None
NM_001026241 NCBI Caenorhabditis elegans F25H5.3d (F25H5.3) mRNA, complete cds. 11/11 None
NM_001026240 NCBI Caenorhabditis elegans F25H5.3c (F25H5.3) mRNA, complete cds. 11/11 None
NM_060058 NCBI Caenorhabditis elegans F25H5.3b (F25H5.3) mRNA, complete cds. 11/11 None
GENEFINDER00000024333 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9178378:9185240:1 11/11 None
SNAP00000024321 ENSEMBL cdna:SNAP chromosome:CEL140:I:9181852:9185240:1 11/11 None
F25H5.3d ENSEMBL cdna:known chromosome:CEL140:I:9176556:9185240:1 gene:F25H5.3 11/11 None
F25H5.3a ENSEMBL cdna:known chromosome:CEL140:I:9176556:9185609:1 gene:F25H5.3 11/11 None
F25H5.3c.1 ENSEMBL cdna:known chromosome:CEL140:I:9181983:9185240:1 gene:F25H5.3 11/11 None
F25H5.3c.2 ENSEMBL cdna:known chromosome:CEL140:I:9182343:9185240:1 gene:F25H5.3 11/11 None
F25H5.3b ENSEMBL cdna:known chromosome:CEL140:I:9182605:9185240:1 gene:F25H5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9182565-9185171(+) 100.0 100.0
Public Domain and Genome References
Ensembl F25H5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02984 Hypothetical protein F25H5.3a [Caenorhabditis elegans] ref|NP_492458.1| F25H5.3a [Caenorhabditis elegans] 0.0
blast CAJ30227 Hypothetical protein F25H5.3d [Caenorhabditis elegans] ref|NP_001021412.1| F25H5.3d [Caenorhabditis elegans] 0.0
blast CAE70385 Hypothetical protein CBG16947 [Caenorhabditis briggsae] 0.0
blast CAE54896 Hypothetical protein F25H5.3c [Caenorhabditis elegans] ref|NP_001021411.1| F25H5.3c [Caenorhabditis elegans] 0.0
blast CAB02983 Hypothetical protein F25H5.3b [Caenorhabditis elegans] ref|NP_492459.1| F25H5.3b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001697 EMBL-EBI Pyruvate kinase 1.2E-44
Pfam IPR001697 EMBL-EBI Pyruvate kinase 1.0E-126
Pfam IPR001697 EMBL-EBI Pyruvate kinase 1.2E-44
Pfam IPR001697 EMBL-EBI Pyruvate kinase 1.0E-126
Pfam IPR001697 EMBL-EBI Pyruvate kinase 1.2E-44
Pfam IPR001697 EMBL-EBI Pyruvate kinase 1.0E-126
Pfam IPR001697 EMBL-EBI Pyruvate kinase 1.2E-44
Pfam IPR001697 EMBL-EBI Pyruvate kinase 1.0E-126
Pfam IPR001697 EMBL-EBI Pyruvate kinase 1.2E-44
Pfam IPR001697 EMBL-EBI Pyruvate kinase 1.0E-126
Pfam IPR001697 EMBL-EBI Pyruvate kinase 1.2E-44
Pfam IPR001697 EMBL-EBI Pyruvate kinase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189418_S_AT
aatcgatggttcacaagccacgtccaaccagagctgaaggatcagatgttgccaatgctg
tgcttgacggagctgattgtgtgatgctttctggagagactgctaaaggagaatatccag
ttgatgcactcaagatcatgcattatatatgcaaagaagccgaagcagcagtttatcatc
gtcgtctcttcgatgaacttcttcaaaacacacaaaaaccaactgacatgtctcatacta
ttgctatcgctgccacatctgcagctgcatcgtgccatgcttcagcaattcttctcatta
ccaccactggacgctcggcaatccaatgctctcgctacaagccagccgtcccaattctga
caatctcgagagacgttgctgtgtgccgtcagcttcatctttacagaggagttttcccag
ttcattatccagctgaacgtgctgccgattggccaactgatgttgataacagaatcaacc
atgctatcgccatcggaaaggatcgtggattcattcacaagggtgacttcttggttgttg
tcactggatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCGATGGTTCACAAGCCACGTCC 231 167 1214 Antisense
GCCACGTCCAACCAGAGCTGAAGGA 669 279 1230 Antisense
TGTTGCCAATGCTGTGCTTGACGGA 464 563 1260 Antisense
TCTCATACTATTGCTATCGCTGCCA 482 613 1444 Antisense
TGCTTCAGCAATTCTTCTCATTACC 514 579 1491 Antisense
CCGTCCCAATTCTGACAATCTCGAG 492 265 1559 Antisense
ATCTCGAGAGACGTTGCTGTGTGCC 538 33 1576 Antisense
GTTTTCCCAGTTCATTATCCAGCTG 260 449 1624 Antisense
CTGAACGTGCTGCCGATTGGCCAAC 610 237 1646 Antisense
CAGAATCAACCATGCTATCGCCATC 180 205 1683 Antisense
ACTTCTTGGTTGTTGTCACTGGATG 371 103 1739 Antisense
Sequence Source Affymetrix Proprietary Database