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[189416_at]

Description F27B3.2 /REP_DB=WormBase Gene ID /WP=CE24923 /TR=Q9N5U8 /GB=AAF39790.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=channel protein
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 09      4grp_high_8_8 > 10      6grp_highest_nonorm > 90      6grp_total > 92      4grp_total > 22      6grp_cgc > 97      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 230
1 2 3 4 range
A 285.7
(2.3)
P 		165.4
(-1.6)
P 		123.9
(-3)
P 		307.8
(3)
P 		184
B 198.5
(2.3)
P 		95.4
(-2.2)
P 		77.6
(-3)
M 		214.8
(3)
P 		137
C 266.6
(2.9)
P 		127.4
(-2.7)
P 		120.7
(-3)
P 		269.4
(3)
P 		149
avr 250.3
(2.5) 		129.4
(-2.2) 		107.4
(-3) 		264
(3) 		157

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 198.8
(3)
P 		134.2
(-2.2)
P 		124.3
(-3)
P 		142.5
(-1.5)
P 		192.5
(2.5)
P 		162.8
(0.1)
P 		75
B 147.6
(-3)
P 		163.6
(-0.3)
P 		157.2
(-1.4)
P 		160.9
(-0.8)
P 		183.7
(3)
P 		155.5
(-1.7)
P 		36
C 141.9
(-1.9)
P 		189.1
(3)
P 		131.7
(-3)
P 		186.9
(2.8)
P 		167.8
(0.8)
P 		138.3
(-2.3)
P 		57
avr 162.8
(0.5) 		162.3
(0.4) 		137.7
(-3) 		163.4
(0.5) 		181.3
(3) 		152.2
(-1) 		44
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 104
(3)
P 		35.9
(-2.7)
P 		32.4
(-3)
P 		45.1
(-1.9)
P 		72
B 63.3
(3)
P 		23.6
(-2.3)
P 		18
(-3)
M 		45.8
(0.7)
P 		45
C 88.3
(3)
P 		36.6
(-3)
P 		42.1
(-2.4)
P 		45.4
(-2)
P 		52
avr 85.2
(3) 		32
(-2.9) 		30.8
(-3) 		45.4
(-1.4) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 36.7
(-1.1)
P 		47.7
(1)
P 		27.1
(-3)
P 		36.1
(-1.3)
P 		58.1
(3)
P 		39.6
(-0.6)
P 		31
B 51.4
(-0.8)
P 		54.4
(-0.3)
P 		38.2
(-3)
P 		42.6
(-2.3)
P 		74.6
(3)
P 		58
(0.3)
P 		36
C 52.6
(1.7)
P 		57.7
(3)
P 		33.5
(-3)
P 		37.6
(-2)
P 		54.3
(2.2)
P 		46.3
(0.2)
P 		24
avr 46.9
(-0.1) 		53.3
(1.2) 		32.9
(-3) 		38.8
(-1.8) 		62.3
(3) 		48
(0.1) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189416_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6906
Sequence Type Exemplar sequence
Representative Public ID F27B3.2 NCBI
Target Description F27B3.2 /REP_DB=WormBase Gene ID /WP=CE24923 /TR=Q9N5U8 /GB=AAF39790.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=channel protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Consensus/Exemplar Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade C annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066051
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000039385 ENSEMBL cdna:SNAP chromosome:CEL140:III:6586102:6586484:1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_066051 4/11 Cross Hyb Matching Probes None
F27B3.2 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6582574-6586483(+) 100.0 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE69177 Hypothetical protein CBG15209 [Caenorhabditis briggsae] 9.0E-34
blast CAE57017 Hypothetical protein CBG24892 [Caenorhabditis briggsae] 1.0E-15
blast AAS75781 neuronal nicotinic acetylcholine Apisa7-2 subunit [Apis mellifera] 2.0E-10
Protein Domains
Database ID Description E-Value
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 3.3E-6
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000039385 1 50-67
Sequence
Target Sequence 
>C. ELEGANS:189416_AT
tatcagccaacgtccattgtaaccccgatgatcggctccttcttggctctcagagttctc
aaaaaaatttaaataaatttgattcggagaaaaacctgcgctcgtcgctatccataagaa
gtggaagcccaacagcaaaaaagaaggtctcattttcctcattgaattcccacggaactc
ccattgattcaccaatttatggtcgaaagcttggaaggactcaatcccaaacaccaactt
cctcatatcgaaattcaaatgggaatggaggaataacatcggatccccttcctcaacagc
tttcgaatcctcccaaaatttcattgactgatgttgatgatgttttcgagaataacgaaa
atcaacagccaatggtcgatgaatttgaaaaagagttcttgagggttatgtcaatggttc
acggaattattgaaagaaatgaaatgagagttgctgaaaaggataaacgagatgcaattg
ctctcgaatggcaacaggttgcaatggtgctcgatcgctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATCAGCCAACGTCCATTGTAACCC 561 655 1406 Antisense
TTGTAACCCCGATGATCGGCTCCTT 590 687 1422 Antisense
TCCTTCTTGGCTCTCAGAGTTCTCA 145 593 1442 Antisense
CGCTCGTCGCTATCCATAAGAAGTG 566 255 1504 Antisense
CATTTTCCTCATTGAATTCCCACGG 512 215 1556 Antisense
ACGGAACTCCCATTGATTCACCAAT 203 97 1577 Antisense
GGAAGGACTCAATCCCAAACACCAA 108 531 1618 Antisense
CAAACACCAACTTCCTCATATCGAA 663 191 1633 Antisense
GAGGAATAACATCGGATCCCCTTCC 2 399 1673 Antisense
GCTTTCGAATCCTCCCAAAATTTCA 650 301 1704 Antisense
AGGTTGCAATGGTGCTCGATCGCTT 465 57 1901 Antisense
Sequence Source Affymetrix Proprietary Database