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[189412_at] cytochrome P450

Description Y17G9B.3 /REP_DB=WormBase Gene ID /WP=CE24183 /TR=Q9N574 /GB=AAF60444.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_total > 96      4grp_total > 0      6grp_cgc > 78      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 33.1
(-3)
A 		47.4
(-1.5)
P 		90.3
(3)
A 		82.9
(2.2)
A 		57
B 44.1
(-2.5)
A 		38.7
(-3)
A 		49.3
(-2)
A 		101.5
(3)
A 		63
C 79.8
(3)
M 		39.1
(-3)
A 		68.8
(1.4)
A 		57.6
(-0.3)
A 		41
avr 52.3
(-1.4) 		41.7
(-3) 		69.5
(1.3) 		80.7
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 61.6
(-1.8)
A 		59.6
(-1.9)
A 		37.5
(-3)
P 		75.4
(-1.1)
M 		157.4
(3)
P 		145.8
(2.4)
P 		120
B 45.3
(-2)
A 		22.9
(-2.9)
A 		21.5
(-3)
A 		67.4
(-1.1)
P 		161.9
(2.8)
P 		166.7
(3)
P 		145
C 15.1
(-3)
A 		35.5
(-2.3)
A 		49.2
(-1.8)
A 		89.7
(-0.3)
P 		130.2
(1.1)
P 		183.7
(3)
P 		169
avr 40.7
(-2.8) 		39.3
(-2.9) 		36.1
(-3) 		77.5
(-1.1) 		149.8
(2.3) 		165.4
(3) 		129
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 12
(-2.2)
A 		10.3
(-3)
P 		23.6
(3)
A 		12.1
(-2.2)
A 		13
B 14.1
(-0.8)
A 		9.6
(-3)
A 		11.4
(-2.1)
A 		21.6
(3)
A 		12
C 26.4
(3)
M 		11.2
(-2.5)
A 		24
(2.1)
A 		9.7
(-3)
A 		17
avr 17.5
(1.6) 		10.4
(-3) 		19.7
(3) 		14.5
(-0.4) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 11.4
(-2.5)
A 		21.2
(-1)
A 		8.2
(-3)
P 		19.1
(-1.3)
M 		47.5
(3)
P 		35.5
(1.2)
P 		39
B 15.7
(-2)
A 		7.6
(-2.8)
A 		5.2
(-3)
A 		17.8
(-1.8)
P 		65.8
(3)
P 		62.2
(2.6)
P 		61
C 5.6
(-3)
A 		10.8
(-2.4)
A 		12.5
(-2.3)
A 		18
(-1.7)
P 		42.1
(0.9)
P 		61.5
(3)
P 		56
avr 10.9
(-2.7) 		13.2
(-2.4) 		8.6
(-3) 		18.3
(-1.7) 		51.8
(2.8) 		53.1
(3) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189412_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10504
Sequence Type Exemplar sequence
Representative Public ID Y17G9B.3 NCBI
Target Description Y17G9B.3 /REP_DB=WormBase Gene ID /WP=CE24183 /TR=Q9N574 /GB=AAF60444.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068236(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068236 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-31A3) (cyp-31A3) mRNA, complete cds. 11/11 None
SNAP00000043800 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4761051:4762778:1 11/11 None
GENEFINDER00000043811 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4761051:4762778:1 11/11 None
Y17G9B.3 ENSEMBL cdna:known chromosome:CEL140:IV:4761051:4762778:1 gene:Y17G9B.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4761056-4762784(+) 100.0 100.0
chrIV:11403948-11405936(+) 92.64 92.64
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-31A3
Ensembl Y17G9B.3
Entrez Gene 189467 Entrez gene
SwissProt Q9N574 EMBL-EBI
WormBase CE24183 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246268_AT cytochrome P450 family protein at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_500637 CYtochrome P450 family member (cyp-31A3) [Caenorhabditis elegans] gb|AAF60444.1| Cytochrome p450 family protein 31A3 [Caenorhabditis elegans] 0.0
blast CAA92741 Hypothetical protein H02I12.8 [Caenorhabditis elegans] emb|CAB07222.3| Hypothetical protein H02I12.8 [Caenorhabditis elegans] ref|NP_502152.3| CYtochrome P450 family member (cyp-31A2) [Caenorhabditis elegans] 0.0
blast CAE58606 Hypothetical protein CBG01773 [Caenorhabditis briggsae] 0.0
blast CAA92741 Hypothetical protein H02I12.8 [Caenorhabditis elegans] emb|CAB07222.3| Hypothetical protein H02I12.8 [Caenorhabditis elegans] ref|NP_502152.3| CYtochrome P450 family member (cyp-31A2) [Caenorhabditis elegans] 1.0E-165
Protein Families
Method ID Description E-Value
p450 prf:1705176A Animalia; CYP2A8; ham; prf:1705176A; Hamster; 1705176A 8.0E-91
p450 prf:1509339A Animalia; CYP2A6; hum; prf:1509339A; Homo sapiens; 1509339A 1.0E-122
p450 CPA6_HUMAN Animalia; CYP2A6; hum; CPA6_HUMAN; Homo sapiens; P11509 1.0E-125
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.0E-122
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.4E-9
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.1E-102
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500637.1 1 2-21
Y17G9B.3 1 2-21
SNAP00000043800 1 2-21
GENEFINDER00000043811 1 2-21
Sequence
Target Sequence 
>C. ELEGANS:189412_AT
tgacacattcatgtttgaaggccacgatacaacatctacaggactaatgtgggcaataca
tcttctgggaaatcatcctgaagttcagagaaaagttcaagcggaattggatgaagtgat
gggcgatgatgaggacgtgactattgaacatctctccagaatgaaatatctcgaatgtgc
actaaaagaagctctccgattgttcccatctgttccaattattacaagagaattgagtga
tgatcaagtgattggaggtgttaatattccaaaaggagtcacttttctgctcaacctgta
ccttgttcatcgtgatccttcgcaatggaaggatcctgatgtattcgatccggatcgttt
ccttcctgaaaattcaattgctcgcaagtctttcgccttcattccattctcagctggaag
tcgtaactgcattggccaacgttttgcactcatggaggagaaggtcatcatggctcattt
gttgagaaatttcaatgtaaaagctgtcgagttgatgcatgaggttcgtccgaaaatgga
gattatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGACACATTCATGTTTGAAGGCCAC 255 571 939 Antisense
GGACTAATGTGGGCAATACATCTTC 257 525 979 Antisense
GCGGAATTGGATGAAGTGATGGGCG 53 293 1039 Antisense
AAGTGATGGGCGATGATGAGGACGT 164 159 1052 Antisense
AAAAGAAGCTCTCCGATTGTTCCCA 194 133 1122 Antisense
GTTCATCGTGATCCTTCGCAATGGA 107 445 1243 Antisense
GGATCGTTTCCTTCCTGAAAATTCA 586 511 1290 Antisense
GTTTCCTTCCTGAAAATTCAATTGC 590 449 1295 Antisense
TCAATTGCTCGCAAGTCTTTCGCCT 640 633 1312 Antisense
GAGTTGATGCATGAGGTTCGTCCGA 688 397 1447 Antisense
GGTTCGTCCGAAAATGGAGATTATC 462 503 1461 Antisense
Sequence Source Affymetrix Proprietary Database