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[189405_at] cytochrome P450

Description F14H3.10 /REP_DB=WormBase Gene ID /WP=CE15834 /TR=O45364 /GB=CAB05484.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_total > 95      4grp_total > 25      6grp_cgc > 88      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 12.2
(-3)
A 		57.3
(-0.1)
A 		22.9
(-2.3)
A 		105.2
(3)
P 		93
B 32.9
(-3)
A 		43.8
(-1.3)
A 		58
(1)
P 		70.5
(3)
P 		38
C 11.8
(-3)
A 		40.7
(0.7)
P 		28.3
(-0.9)
A 		58.7
(3)
A 		47
avr 19
(-3) 		47.3
(-0.1) 		36.4
(-1.2) 		78.1
(3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 43.6
(0.8)
A 		14
(-3)
A 		26.7
(-1.4)
A 		26.9
(-1.3)
A 		60.5
(3)
A 		45.7
(1.1)
P 		47
B 27.6
(0)
A 		32.6
(0.7)
A 		49.1
(3)
A 		6.1
(-3)
A 		20.1
(-1)
M 		18.7
(-1.2)
P 		43
C 5.6
(-2.6)
A 		9.1
(-2.2)
A 		1.9
(-3)
A 		53.9
(3)
A 		32.3
(0.5)
A 		28.6
(0.1)
P 		52
avr 25.6
(-0.8) 		18.6
(-3) 		25.9
(-0.7) 		29
(0.3) 		37.6
(3) 		31
(0.9) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 4.5
(-3)
A 		12.4
(1.3)
A 		6
(-2.2)
A 		15.4
(3)
P 		11
B 10.5
(-3)
A 		10.8
(-2.6)
A 		13.4
(0.9)
P 		15
(3)
P 		5
C 3.9
(-3)
A 		11.7
(3)
P 		9.8
(1.5)
A 		9.9
(1.6)
A 		8
avr 6.3
(-3) 		11.6
(1.5) 		9.7
(-0.1) 		13.4
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 8
(-1.6)
A 		5
(-3)
A 		5.8
(-2.6)
A 		6.8
(-2.2)
A 		18.2
(3)
A 		11.1
(-0.2)
P 		13
B 9.6
(1.7)
A 		10.9
(2.4)
A 		11.9
(3)
A 		1.6
(-3)
A 		8.2
(0.8)
M 		7
(0.1)
P 		10
C 2.1
(-2.1)
A 		2.8
(-1.7)
A 		0.5
(-3)
A 		10.8
(3)
A 		10.5
(2.8)
A 		9.6
(2.3)
P 		10
avr 6.6
(-2.5) 		6.2
(-2.9) 		6.1
(-3) 		6.4
(-2.7) 		12.3
(3) 		9.2
(-0) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189405_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13322
Sequence Type Exemplar sequence
Representative Public ID F14H3.10 NCBI
Target Description F14H3.10 /REP_DB=WormBase Gene ID /WP=CE15834 /TR=O45364 /GB=CAB05484.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074643(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074643 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-35D1) (cyp-35D1) mRNA, complete cds. 11/11 None
SNAP00000013828 ENSEMBL cdna:SNAP chromosome:CEL140:V:16072742:16073842:1 11/11 None
GENEFINDER00000013832 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16071838:16073842:1 11/11 None
F14H3.10 ENSEMBL cdna:known chromosome:CEL140:V:16071838:16073842:1 gene:F14H3.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16071837-16073842(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-35D1
Ensembl F14H3.10
Entrez Gene 184495 Entrez gene
SwissProt O45364 EMBL-EBI
WormBase CE15834 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05484 Hypothetical protein F14H3.10 [Caenorhabditis elegans] ref|NP_507044.1| CYtochrome P450 family member (cyp-35D1) [Caenorhabditis elegans] 0.0
blast CAE74942 Hypothetical protein CBG22831 [Caenorhabditis briggsae] 0.0
blast CAE58284 Hypothetical protein CBG01391 [Caenorhabditis briggsae] 1.0E-148
blast CAB05484 Hypothetical protein F14H3.10 [Caenorhabditis elegans] ref|NP_507044.1| CYtochrome P450 family member (cyp-35D1) [Caenorhabditis elegans] 1.0E-148
Protein Families
Method ID Description E-Value
p450 CPA2_RAT Animalia; CYP2A2; rat; CPA2_RAT; Rattus sp.; P15149 1.0E-67
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.2E-78
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.5E-41
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507044.1 1 2-19
F14H3.10 1 2-19
GENEFINDER00000013832 1 2-19
Sequence
Target Sequence 
>C. ELEGANS:189405_AT
gaaccactgccacaacattgtacgtgggattcatgaaacttgtgaatcaccctggaatta
tacaaaatattcaaaaggagctcctcgacatcactgaaaatggagcaagagatttgactc
taaaagatagaccgaacacaccatatttgaatgcgacgattgcagaaattcagagacacg
cttcgattttaaatgtaaatttctggaggatcaatcacgagactatacattttaatgact
atcaagtggatccgggaacaatgatcgccgcacaagttggagtccttcatgtcaacgagg
aactgtttgataatccaaaggattttaatgtagaaaagtacttgaaaaacccgaagcttc
tgcagcaagtgatcccattcggaattggcaagaggtcctgcgtcggggagcagattgcaa
aatcggaactttacctggttttcgggaacatccttctccgttacaatgttaaaaaacatg
gttcaactccaaccaacgaagacgtgtacccatatagttctgcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACCACTGCCACAACATTGTACGT 585 345 944 Antisense
CAAAAGGAGCTCCTCGACATCACTG 264 191 1015 Antisense
AATTCAGAGACACGCTTCGATTTTA 81 177 1110 Antisense
GATCCGGGAACAATGATCGCCGCAC 168 417 1192 Antisense
GCCGCACAAGTTGGAGTCCTTCATG 250 285 1210 Antisense
AAAACCCGAAGCTTCTGCAGCAAGT 404 131 1289 Antisense
GCAAGTGATCCCATTCGGAATTGGC 624 319 1308 Antisense
GAATTGGCAAGAGGTCCTGCGTCGG 526 321 1325 Antisense
GAACATCCTTCTCCGTTACAATGTT 260 351 1389 Antisense
TCAACTCCAACCAACGAAGACGTGT 322 627 1426 Antisense
GACGTGTACCCATATAGTTCTGCAA 226 377 1444 Antisense
Sequence Source Affymetrix Proprietary Database