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[189404_at] protein kinase

Description Y52D5A.2 /REP_DB=WormBase Gene ID /WP=CE26155 /TR=Q9N4S6 /GB=AAF60752.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 95      4grp_total > 16      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 9.9
(-3)
A 		25.7
(-1.9)
A 		16.2
(-2.5)
A 		93.6
(3)
A 		84
B 11.1
(-3)
A 		36
(1.4)
A 		44.9
(3)
A 		12.1
(-2.8)
A 		34
C 33.6
(3)
A 		7.4
(-3)
A 		12.6
(-1.8)
A 		12.3
(-1.9)
A 		26
avr 18.2
(-3) 		23
(-1.6) 		24.6
(-1.2) 		39.3
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 8.9
(-3)
A 		21.3
(-1.4)
A 		23.7
(-1.1)
A 		9.9
(-2.9)
A 		25.3
(-0.9)
A 		54.8
(3)
A 		46
B 60.8
(3)
A 		14.9
(-3)
A 		39.8
(0.3)
A 		48.8
(1.4)
A 		50.3
(1.6)
A 		39.8
(0.3)
A 		46
C 7.7
(-2.9)
A 		8.6
(-2.8)
A 		7.3
(-3)
A 		45.1
(3)
A 		32
(0.9)
A 		10.2
(-2.5)
A 		38
avr 25.8
(0.1) 		14.9
(-3) 		23.6
(-0.5) 		34.6
(2.6) 		35.9
(3) 		34.9
(2.7) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 3.6
(-3)
A 		5.6
(-1.8)
A 		4.2
(-2.6)
A 		13.7
(3)
A 		10
B 3.5
(-2.3)
A 		8.9
(1.8)
A 		10.4
(3)
A 		2.6
(-3)
A 		8
C 11.1
(3)
A 		2.1
(-3)
A 		4.4
(-1.5)
A 		2.1
(-3)
A 		9
avr 6.1
(1.5) 		5.5
(-3) 		6.3
(3) 		6.1
(1.5) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 1.6
(-3)
A 		7.6
(0.1)
A 		5.2
(-1.2)
A 		2.5
(-2.5)
A 		7.6
(0.1)
A 		13.3
(3)
A 		12
B 21.2
(3)
A 		4.9
(-3)
A 		9.7
(-1.2)
A 		12.9
(-0.1)
A 		20.4
(2.7)
A 		14.8
(0.6)
A 		16
C 2.8
(-2.4)
A 		2.6
(-2.5)
A 		1.9
(-3)
A 		9.1
(2.1)
A 		10.4
(3)
A 		3.4
(-1.9)
A 		9
avr 8.5
(-0.3) 		5
(-3) 		5.6
(-2.5) 		8.2
(-0.5) 		12.8
(3) 		10.5
(1.2) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189404_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10729
Sequence Type Exemplar sequence
Representative Public ID Y52D5A.2 NCBI
Target Description Y52D5A.2 /REP_DB=WormBase Gene ID /WP=CE26155 /TR=Q9N4S6 /GB=AAF60752.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068412(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068412 NCBI Caenorhabditis elegans Y52D5A.2 (Y52D5A.2) mRNA, complete cds. 11/11 None
SNAP00000019800 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5462488:5468931:-1 11/11 None
GENEFINDER00000019805 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5462580:5464642:-1 9/11 None
Y52D5A.2 ENSEMBL cdna:known chromosome:CEL140:IV:5462488:5464262:-1 gene:Y52D5A.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5462493-5464783(-) 98.87 100.0
Public Domain and Genome References
Gene Title protein kinase
Ensembl Y52D5A.2
Entrez Gene 190185 Entrez gene
SwissProt Q9N4S6 EMBL-EBI
WormBase CE36750 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60752 Hypothetical protein Y52D5A.2 [Caenorhabditis elegans] ref|NP_500813.2| Y52D5A.2 [Caenorhabditis elegans] 0.0
blast CAA22261 Hypothetical protein Y69E1A.3 [Caenorhabditis elegans] ref|NP_502040.1| Y69E1A.3 [Caenorhabditis elegans] 0.0
blast CAA94373 Hypothetical protein T25B9.5 [Caenorhabditis elegans] ref|NP_501993.1| T25B9.5 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.0E-45
Pfam IPR000719 EMBL-EBI Protein kinase 1.0E-45
Pfam IPR000719 EMBL-EBI Protein kinase 1.0E-45
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000019805 1 13-32
Sequence
Target Sequence 
>C. ELEGANS:189404_AT
agagcccctctcagatggatggcaccggagagtttcaattctggaacgttcaatgaaaag
acagatgtatggtcttatggagtgcttttgacggagctcatgacaagatgtgagcacgat
ccactgtacccgatgagtctgaaagatgcgaaggcatggatagcctcagaaagtttccct
catagaattgtgaatggtgatccgaaggaactgatgagtctcgtggatgcgtgctgtgat
aaaaacgtgaaagaccggatcaagttcaacacggtgaagcgacgaatctctgacatctat
tccgaggcaatgagcaaagcttccgttcaggatggtcagatgccatttacctcaactgct
gttgctttagaagcgaaaccatcagaagaacgaacaaaaatagcaccttcattgaatcgg
aagaaatctgccgataagcagaggatccattcaatacggcgactcttcccatcacttcgt
cgtaaaaacaaggatcaagtcatactgccagccggaatttctgtcggcccatcaagtaat
tcaaccaaaccaccatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGCCCCTCTCAGATGGATGGCAC 12 69 973 Antisense
GGATGGCACCGGAGAGTTTCAATTC 439 515 989 Antisense
ATCCACTGTACCCGATGAGTCTGAA 84 41 1091 Antisense
GATAGCCTCAGAAAGTTTCCCTCAT 260 425 1131 Antisense
AGTCTCGTGGATGCGTGCTGTGATA 383 61 1189 Antisense
GAAGCGACGAATCTCTGACATCTAT 654 343 1248 Antisense
GCAAAGCTTCCGTTCAGGATGGTCA 179 317 1286 Antisense
GCCATTTACCTCAACTGCTGTTGCT 97 281 1314 Antisense
GATCCATTCAATACGGCGACTCTTC 66 417 1416 Antisense
GAATTTCTGTCGGCCCATCAAGTAA 234 325 1487 Antisense
AAGTAATTCAACCAAACCACCATCA 269 157 1506 Antisense
Sequence Source Affymetrix Proprietary Database