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[189400_s_at] Protein kinase

Description F28B12.3 /REP_DB=WormBase Gene ID /WP=CE02704 /TR=Q19848 /GB=AAK31507.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Protein kinase
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 78      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 92      6grp_total > 69      4grp_total > 47      6grp_highest_sub > Da      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 213
1 2 3 4 range
A 364.8
(3)
P 		234.7
(-3)
P 		255.6
(-2)
P 		269.4
(-1.4)
P 		130
B 405.2
(3)
P 		289.2
(-2.3)
P 		274.3
(-3)
P 		274
(-3)
P 		131
C 329
(3)
P 		192.7
(-3)
P 		260
(-0)
P 		252.3
(-0.4)
P 		136
avr 366.3
(3) 		238.9
(-3) 		263.3
(-1.9) 		265.2
(-1.8) 		127

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 465
1 2 3 4 5 6 range
A 383.8
(0)
P 		286
(-1.3)
P 		150.7
(-3)
P 		298.8
(-1.1)
P 		280.9
(-1.3)
P 		615.9
(3)
P 		465
B 231.8
(-1.4)
P 		256
(-0.7)
P 		168.7
(-3)
P 		218.3
(-1.7)
P 		276.5
(-0.2)
P 		401
(3)
P 		232
C 307.9
(-2.1)
P 		275.2
(-3)
P 		317.5
(-1.8)
P 		328.5
(-1.5)
P 		387.1
(0.1)
P 		490.2
(3)
P 		215
avr 307.8
(-1) 		272.4
(-1.8) 		212.3
(-3) 		281.9
(-1.6) 		314.8
(-0.9) 		502.4
(3) 		290
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 132.8
(3)
P 		51
(-2.3)
P 		66.8
(-1.2)
P 		39.4
(-3)
P 		93
B 129.2
(3)
P 		71.4
(-1.9)
P 		63.6
(-2.6)
P 		58.4
(-3)
P 		71
C 108.9
(3)
P 		55.4
(-1.8)
P 		90.6
(1.3)
P 		42.5
(-3)
P 		66
avr 123.6
(3) 		59.3
(-2) 		73.7
(-0.9) 		46.8
(-3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 70.8
(-1.1)
P 		101.7
(0.5)
P 		32.8
(-3)
P 		75.8
(-0.8)
P 		84.8
(-0.3)
P 		149.9
(3)
P 		117
B 80.7
(-0.8)
P 		85.2
(-0.6)
P 		41
(-3)
P 		57.8
(-2.1)
P 		112.3
(0.9)
P 		149.5
(3)
P 		109
C 114.1
(-0.1)
P 		84
(-1.9)
P 		80.8
(-2.1)
P 		66.1
(-3)
P 		125.3
(0.6)
P 		164.1
(3)
P 		98
avr 88.5
(-0.8) 		90.3
(-0.7) 		51.5
(-3) 		66.6
(-2.1) 		107.5
(0.3) 		154.5
(3) 		103
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189400_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4472
Sequence Type Exemplar sequence
Representative Public ID F28B12.3 NCBI
Target Description F28B12.3 /REP_DB=WormBase Gene ID /WP=CE02704 /TR=Q19848 /GB=AAK31507.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062784(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062784 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-223) (tag-223) mRNA, complete cds. 11/11 None
SNAP00000017904 ENSEMBL cdna:SNAP chromosome:CEL140:II:5921935:5924354:1 9/11 None
GENEFINDER00000017914 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5921935:5924421:1 11/11 None
F28B12.3.1 ENSEMBL cdna:known chromosome:CEL140:II:5921930:5924801:1 gene:F28B12.3 11/11 None
F28B12.3.2 ENSEMBL cdna:known chromosome:CEL140:II:5921934:5924801:1 gene:F28B12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5921931-5924418(+) 100.0 100.0
Public Domain and Genome References
Gene Title Protein kinase
Gene Symbol vrk-1
Ensembl F28B12.3
Entrez Gene 174004 Entrez gene
SwissProt Q19848 EMBL-EBI
WormBase CE02704 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1586870_AT similar to vaccinia related kinase 1 cfa
CANINE_2:CFA.2967.1.A1_AT similar to vaccinia related kinase 1 cfa
CANINE_2:CFAAFFX.27237.1.S1_AT similar to vaccinia related kinase 1 cfa
CANINE_2:CFAAFFX.27241.1.S1_S_AT similar to vaccinia related kinase 1 cfa
DROSOPHILA_2:1628342_S_AT ballchen dm
DROSGENOME1:153644_AT ballchen dm
CHICKEN:GGA.11797.1.S1_S_AT vaccinia related kinase 1 gga
CHICKEN:GGAAFFX.11571.1.S1_AT vaccinia related kinase 1 gga
HU35KSUBB:RC_AA112979_S_AT vaccinia related kinase 1 hs
HUGENEFL:AB000449_AT vaccinia related kinase 1 hs
HG-U133A:203856_AT vaccinia related kinase 1 hs
HG-U133_PLUS_2:203856_AT vaccinia related kinase 1 hs
HG-FOCUS:203856_AT vaccinia related kinase 1 hs
HG-U133A_2:203856_AT vaccinia related kinase 1 hs
U133_X3P:G4507902_3P_AT vaccinia related kinase 1 hs
HG-U95AV2:39980_AT vaccinia related kinase 1 hs
HG-U95B:59179_AT Vaccinia related kinase 1 hs
HG-U95D:82290_AT Vaccinia related kinase 1 hs
HU35KSUBA:RC_AA007522_AT Vaccinia related kinase 1 hs
MOE430A:1425006_A_AT vaccinia related kinase 1 mm
MOUSE430_2:1425006_A_AT vaccinia related kinase 1 mm
MOUSE430A_2:1425006_A_AT vaccinia related kinase 1 mm
MG-U74AV2:97393_AT vaccinia related kinase 1 mm
MOE430B:1458951_AT vaccinia related kinase 1 mm
MOUSE430_2:1458951_AT vaccinia related kinase 1 mm
MU11KSUBA:AA266513_AT Vaccinia related kinase 1, transcript variant 3, mRNA (cDNA clone MGC:12003 IMAGE:3602292) mm
RAE230B:1392168_AT vaccinia related kinase 1 (predicted) rn
RAT230_2:1392168_AT vaccinia related kinase 1 (predicted) rn
RG-U34B:RC_AA924442_AT vaccinia related kinase 1 (predicted) rn
RAE230B:1380009_AT vaccinia related kinase 1 (predicted) rn
RAT230_2:1380009_AT vaccinia related kinase 1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31507 Hypothetical protein F28B12.3 [Caenorhabditis elegans] ref|NP_495185.1| Temporarily Assigned Gene name family member (tag-223) [Caenorhabditis elegans] 0.0
blast CAE59222 Hypothetical protein CBG02540 [Caenorhabditis briggsae] 0.0
blast CAE59222 Hypothetical protein CBG02540 [Caenorhabditis briggsae] 1.0E-149
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.9E-10
Sequence
Target Sequence 
>C. ELEGANS:189400_S_AT
gaaatcatcattccgaagtcgtcaagatctcgaagtaaagtacaattatctggagaagga
tcagatgtcacgacgccgtcttcagctgcatctacttcgagaagtcgcagtattcgtcta
ggattaacgtcatcaactgcttcttctaataaagttgccaaaaaaatcgagcaaaagtac
aagcgtttgtctatgaacaaatcttctctcgttcccgtaacaatttctgttgcatccgat
aaatctccaacaacctcaactccttcgagttcgtctggtctcagatccaaaagaaaatcc
agtgaagatgttggcgaaggaataactctgaagacacaagtgctaattactccagctatt
aaaaaagcgaagacaaagagtggaatttcttcagcaacaaaagcaagcccaaccgagttg
agaagagttcctggcgtcagaaattttccaaaaggcagacgatcgatgattatcaaagag
accagtgcgaagtataaagaacgtcttgcttctcgtcaaacaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATCATCATTCCGAAGTCGTCAA 110 363 1282 Antisense
AAGGATCAGATGTCACGACGCCGTC 227 161 1337 Antisense
AAGTCGCAGTATTCGTCTAGGATTA 389 157 1383 Antisense
TTAACGTCATCAACTGCTTCTTCTA 492 683 1405 Antisense
ATGAACAAATCTTCTCTCGTTCCCG 179 45 1474 Antisense
CTCTCGTTCCCGTAACAATTTCTGT 148 231 1487 Antisense
ATCCGATAAATCTCCAACAACCTCA 673 37 1515 Antisense
ACAACCTCAACTCCTTCGAGTTCGT 87 113 1531 Antisense
TTCGAGTTCGTCTGGTCTCAGATCC 516 695 1545 Antisense
GTTGAGAAGAGTTCCTGGCGTCAGA 21 439 1698 Antisense
ACGTCTTGCTTCTCGTCAAACAAAG 339 93 1782 Antisense
Sequence Source Affymetrix Proprietary Database