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[189399_at] protein kinase

Description H12I13.1 /REP_DB=WormBase Gene ID /WP=CE23804 /TR=Q9N5M9 /GB=AAF39881.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 70      4grp_total > 18      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 3.2
(-3)
A 		8.4
(-2.4)
A 		4.7
(-2.8)
A 		53.8
(3)
A 		51
B 3.6
(-3)
A 		10.8
(-0.2)
A 		5.5
(-2.3)
A 		18.9
(3)
A 		15
C 5
(-2.5)
A 		12.1
(3)
A 		4.3
(-3)
A 		5
(-2.5)
A 		8
avr 3.9
(-3) 		10.4
(-1.2) 		4.8
(-2.8) 		25.9
(3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 5.4
(-2.6)
A 		3.4
(-3)
A 		5
(-2.7)
A 		33.2
(3)
A 		3.3
(-3)
A 		22.3
(0.8)
A 		30
B 6.6
(-1.7)
A 		18.7
(3)
A 		15.1
(1.6)
A 		6
(-1.9)
A 		3.3
(-3)
A 		6.1
(-1.9)
A 		15
C 3.5
(-2)
A 		9.8
(3)
A 		2.5
(-2.8)
A 		4.7
(-1.1)
A 		7.2
(0.9)
A 		2.3
(-3)
A 		8
avr 5.2
(-2.6) 		10.6
(0.6) 		7.5
(-1.3) 		14.6
(3) 		4.6
(-3) 		10.2
(0.4) 		10
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 1.2
(-3)
A 		1.8
(-2.5)
A 		1.2
(-3)
A 		7.9
(3)
A 		7
B 1.1
(-3)
A 		2.7
(0.3)
A 		1.3
(-2.6)
A 		4
(3)
A 		3
C 1.7
(-1)
A 		3.5
(3)
A 		1.5
(-1.4)
A 		0.8
(-3)
A 		3
avr 1.3
(-3) 		2.7
(-0.1) 		1.3
(-3) 		4.2
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 1
(-3)
A 		1.2
(-2.8)
A 		1.1
(-2.9)
A 		8.4
(3)
A 		1
(-3)
A 		5.4
(0.6)
A 		7
B 2.3
(-1.8)
A 		6.2
(3)
A 		3.7
(-0.1)
A 		1.6
(-2.6)
A 		1.3
(-3)
A 		2.3
(-1.8)
A 		5
C 1.3
(-1.3)
A 		3
(3)
A 		0.6
(-3)
A 		0.9
(-2.3)
A 		2.3
(1.3)
A 		0.8
(-2.5)
A 		2
avr 1.5
(-3) 		3.5
(2.7) 		1.8
(-2.1) 		3.6
(3) 		1.5
(-3) 		2.8
(0.7) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189399_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5926
Sequence Type Exemplar sequence
Representative Public ID H12I13.1 NCBI
Target Description H12I13.1 /REP_DB=WormBase Gene ID /WP=CE23804 /TR=Q9N5M9 /GB=AAF39881.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062811(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062811 NCBI Caenorhabditis elegans H12I13.1 (H12I13.1) mRNA, complete cds. 11/11 None
SNAP00000034373 ENSEMBL cdna:SNAP chromosome:CEL140:II:6059759:6067093:-1 11/11 None
GENEFINDER00000034379 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6059817:6064294:-1 11/11 None
H12I13.1 ENSEMBL cdna:known chromosome:CEL140:II:6062213:6064294:-1 gene:H12I13.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6062209-6065862(-) 89.99 99.55
Public Domain and Genome References
Gene Title protein kinase
Ensembl H12I13.1
Entrez Gene 186732 Entrez gene
SwissProt Q9N5M9 EMBL-EBI
WormBase CE23804 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF39881 Hypothetical protein H12I13.1 [Caenorhabditis elegans] ref|NP_495212.1| H12I13.1 [Caenorhabditis elegans] 0.0
blast AAF39880 Hypothetical protein H12I13.2 [Caenorhabditis elegans] ref|NP_495213.1| H12I13.2 [Caenorhabditis elegans] 0.0
blast AAK31453 Hypothetical protein C25H3.8 [Caenorhabditis elegans] ref|NP_495110.2| C25H3.8 [Caenorhabditis elegans] 4.0E-82
blast T15626 hypothetical protein C25H3.8 - Caenorhabditis elegans 4.0E-82
blast AAK31453 Hypothetical protein C25H3.8 [Caenorhabditis elegans] ref|NP_495110.2| C25H3.8 [Caenorhabditis elegans] 7.0E-79
blast AAG00045 Hypothetical protein ZK177.2 [Caenorhabditis elegans] ref|NP_495058.1| ZK177.2 [Caenorhabditis elegans] sp|Q09371|YS42_CAEEL Hypothetical protein ZK177.2 in chromosome II 3.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR009543 EMBL-EBI Protein of unknown function DUF1162 1.5E-11
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 3.6E-11
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 1.0E-12
Pfam IPR009543 EMBL-EBI Protein of unknown function DUF1162 1.5E-11
Sequence
Target Sequence 
>C. ELEGANS:189399_AT
gagcctatggatcggcatactctgtgaagtacggtgggaaaactgtcgtgatgaagctca
taggaatccgggacaacgagcaactcctcttcaatgaactcatcataacacaacagttgg
gaaaactcgcgaaaaacagagtttgtccgaatttccttgaatattgtggatcccatgtga
tgaccgacgtgcctgttgggatgagaagagatccaaaagcgatacgacacttggcaatat
tgatggctcgtggaggaggagttctggacaattggaagtttaaagactaccgtcaatgtg
tctcagtattctgccagttggttatgtcgttaaaagtggtcaaggattcaataaatatgg
tgcatcgggacatccatttgtggaacgtcctcgtttcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCCTATGGATCGGCATACTCTGT 100 385 1283 Antisense
GGAATCCGGGACAACGAGCAACTCC 371 527 1345 Antisense
GAGCAACTCCTCTTCAATGAACTCA 298 391 1360 Antisense
ACAGAGTTTGTCCGAATTTCCTTGA 335 107 1418 Antisense
TGTGGATCCCATGTGATGACCGACG 399 555 1447 Antisense
ACCGACGTGCCTGTTGGGATGAGAA 12 85 1465 Antisense
AAAGCGATACGACACTTGGCAATAT 142 123 1498 Antisense
GACTACCGTCAATGTGTCTCAGTAT 9 373 1567 Antisense
GTCTCAGTATTCTGCCAGTTGGTTA 51 469 1582 Antisense
TGGTGCATCGGGACATCCATTTGTG 70 553 1640 Antisense
CCATTTGTGGAACGTCCTCGTTTCG 131 271 1656 Antisense
Sequence Source Affymetrix Proprietary Database