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[189397_at] protein kinase

Description C16A11.3 /REP_DB=WormBase Gene ID /WP=CE19353 /TR=O76581 /GB=AAK31409.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 85      4grp_total > 09      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 15.7
(-3)
A 		33.3
(-1)
A 		34.2
(-0.9)
A 		67.6
(3)
A 		52
B 10.9
(-1.9)
A 		3
(-3)
A 		12.3
(-1.7)
A 		46.1
(3)
A 		43
C 7.6
(-2)
A 		11.1
(-1.5)
A 		2
(-3)
A 		37.3
(3)
A 		35
avr 11.4
(-3) 		15.8
(-2.3) 		16.2
(-2.3) 		50.3
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 1.9
(-2.6)
A 		2.1
(-2.5)
A 		0.6
(-3)
A 		18.1
(3)
A 		2.7
(-2.3)
A 		15.3
(2)
A 		18
B 7.6
(-0.4)
A 		5.7
(-1.2)
A 		6.4
(-0.9)
A 		1.7
(-3)
A 		6.1
(-1.1)
A 		15.4
(3)
A 		14
C 14.3
(0)
A 		8.2
(-1.5)
A 		2.4
(-3)
A 		26.1
(3)
A 		21.7
(1.9)
A 		17.4
(0.8)
A 		24
avr 7.9
(-0.8) 		5.3
(-2) 		3.1
(-3) 		15.3
(2.7) 		10.2
(0.3) 		16
(3) 		13
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 5.7
(-3)
A 		7.2
(-0.9)
A 		8.9
(1.6)
A 		9.9
(3)
A 		4
B 3.5
(-1.2)
A 		0.7
(-3)
A 		2.8
(-1.6)
A 		9.8
(3)
A 		9
C 2.5
(-1.1)
A 		3.2
(-0.3)
A 		0.7
(-3)
A 		6.3
(3)
A 		6
avr 3.9
(-2.8) 		3.7
(-3) 		4.1
(-2.5) 		8.7
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 0.4
(-2.6)
A 		0.7
(-2.2)
A 		0.1
(-3)
A 		4.6
(3)
A 		0.8
(-2.1)
A 		3.7
(1.8)
A 		5
B 2.6
(-0.5)
A 		1.9
(-1.3)
A 		1.5
(-1.8)
A 		0.4
(-3)
A 		2.5
(-0.6)
A 		5.7
(3)
A 		5
C 5.3
(1.4)
A 		2.5
(-1.2)
A 		0.6
(-3)
A 		5.3
(1.4)
A 		7
(3)
A 		5.8
(1.9)
A 		6
avr 2.8
(-0.1) 		1.7
(-1.6) 		0.7
(-3) 		3.4
(0.7) 		3.4
(0.7) 		5.1
(3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189397_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4326
Sequence Type Exemplar sequence
Representative Public ID C16A11.3 NCBI
Target Description C16A11.3 /REP_DB=WormBase Gene ID /WP=CE19353 /TR=O76581 /GB=AAK31409.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062344(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062344 NCBI Caenorhabditis elegans C16A11.3 (C16A11.3) mRNA, complete cds. 11/11 A 
SNAP00000042940 ENSEMBL cdna:SNAP chromosome:CEL140:II:4229545:4234317:1 11/11 None
GENEFINDER00000042949 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4231379:4234317:1 11/11 None
C16A11.3 ENSEMBL cdna:known chromosome:CEL140:II:4229545:4234610:1 gene:C16A11.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075674 6/11 Cross Hyb Matching Probes A 
SNAP00000022303 6/11 Cross Hyb Matching Probes None
Y73B3A.16 6/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4229543-4234316(+) 100.0 100.0
Public Domain and Genome References
Gene Title protein kinase
Ensembl C16A11.3
Entrez Gene 173756 Entrez gene
SwissProt O76581 EMBL-EBI
WormBase CE19353 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31409 Hypothetical protein C16A11.3 [Caenorhabditis elegans] ref|NP_494745.1| C16A11.3 [Caenorhabditis elegans] 0.0
blast AAK39370 Hypothetical protein Y73B3A.16 [Caenorhabditis elegans] ref|NP_508075.2| Y73B3A.16 [Caenorhabditis elegans] 1.0E-148
blast AAK39370 Hypothetical protein Y73B3A.16 [Caenorhabditis elegans] ref|NP_508075.2| Y73B3A.16 [Caenorhabditis elegans] 1.0E-147
blast CAC35832 Hypothetical protein Y111B2A.1 [Caenorhabditis elegans] ref|NP_499631.2| Y111B2A.1 [Caenorhabditis elegans] 1.0E-107
Protein Families
Method ID Description E-Value
hanks 3.1.5 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk6 6.0E-16
hanks 3.1.5 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk6 8.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR006570 EMBL-EBI SPK 2.1E-37
Pfam IPR000719 EMBL-EBI Protein kinase 7.9E-9
Pfam IPR000719 EMBL-EBI Protein kinase 9.1E-35
Pfam IPR000719 EMBL-EBI Protein kinase 4.5E-6
Pfam IPR000719 EMBL-EBI Protein kinase 9.1E-35
Pfam IPR000719 EMBL-EBI Protein kinase 7.9E-9
Pfam IPR000719 EMBL-EBI Protein kinase 9.1E-35
Sequence
Target Sequence 
>C. ELEGANS:189397_AT
ttggttgtattcttagtgagctctacactggcgacctgctcttttacggaaattcgaaaa
gtgacacggaagagcttcaattcgaattaatgcagatgattgttcaacaacctccgtcgt
gtgccatgttgagagaagccgaaaaaatcaaaagcacaaaaattcggattgaaaacggca
atgtgtacatgaagcggaaatcgaaatcatcgtttgagccgccaaagccgcttcataagc
aacgacgtccaaaggatttagaagcgattccactattcgaccttcttgaatgtattctca
tcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGTTGTATTCTTAGTGAGCTCTA 428 707 1832 Antisense
TATTCTTAGTGAGCTCTACACTGGC 338 661 1839 Antisense
GACCTGCTCTTTTACGGAAATTCGA 494 383 1864 Antisense
GAAAAGTGACACGGAAGAGCTTCAA 110 355 1887 Antisense
GGAAGAGCTTCAATTCGAATTAATG 233 531 1899 Antisense
GAATTAATGCAGATGATTGTTCAAC 127 325 1915 Antisense
CCTCCGTCGTGTGCCATGTTGAGAG 547 267 1942 Antisense
GTGTGCCATGTTGAGAGAAGCCGAA 395 487 1950 Antisense
GAAGCCGAAAAAATCAAAAGCACAA 15 345 1966 Antisense
CCACTATTCGACCTTCTTGAATGTA 262 275 2101 Antisense
GACCTTCTTGAATGTATTCTCATCG 574 383 2110 Antisense
Sequence Source Affymetrix Proprietary Database