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[189390_at] Cytochrome P450

Description B0304.3 /REP_DB=WormBase Gene ID /WP=CE27050 /TR=Q27465 /GB=AAK31395.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Cytochrome P450
Cluster 6grp_high1_10_10 > 91      6grp_total > 92      4grp_total > 49      6grp_cgc > 97      6grp_high1_sub > 05      6grp_rule_based > S5      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 33.7
(-2.4)
A 		91.4
(3)
P 		88.5
(2.7)
P 		27.4
(-3)
A 		64
B 11.9
(-3)
A 		82.7
(3)
P 		63.2
(1.3)
P 		35.8
(-1)
P 		71
C 39.9
(-3)
M 		90.4
(0.5)
P 		125.7
(3)
P 		73.5
(-0.7)
P 		86
avr 28.5
(-3) 		88.2
(2.6) 		92.5
(3) 		45.6
(-1.4) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 346
1 2 3 4 5 6 range
A 103.5
(-0.5)
P 		83
(-1.1)
P 		116.9
(-0.1)
P 		220.5
(3)
P 		210.1
(2.7)
P 		21.4
(-3)
A 		199
B 96.3
(-2.2)
P 		110.8
(-1.9)
P 		82.8
(-2.4)
P 		164.5
(-0.9)
P 		367.2
(3)
P 		52.7
(-3)
P 		315
C 87.9
(-1.7)
P 		98.7
(-1.4)
P 		86.3
(-1.7)
P 		192.8
(1.1)
P 		262
(3)
P 		38.2
(-3)
P 		224
avr 95.9
(-1.6) 		97.5
(-1.5) 		95.3
(-1.6) 		192.6
(0.8) 		279.8
(3) 		37.4
(-3) 		242
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 12.3
(-0.4)
A 		19.8
(2)
P 		23.1
(3)
P 		4
(-3)
A 		19
B 3.8
(-3)
A 		20.4
(3)
P 		14.6
(0.9)
P 		7.6
(-1.6)
P 		17
C 13.2
(-2.8)
M 		26
(-0.4)
P 		43.8
(3)
P 		12.4
(-3)
P 		31
avr 9.8
(-2.4) 		22.1
(1.4) 		27.2
(3) 		8
(-3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 144
1 2 3 4 5 6 range
A 19.1
(-1.6)
P 		29.5
(-0.5)
P 		25.5
(-0.9)
P 		55.9
(2.2)
P 		63.4
(3)
P 		5.2
(-3)
A 		58
B 33.5
(-2.4)
P 		36.9
(-2.2)
P 		20.1
(-3)
P 		43.6
(-1.9)
P 		149.2
(3)
P 		19.6
(-3)
P 		130
C 32.6
(-1.4)
P 		30.1
(-1.6)
P 		22
(-2.2)
P 		38.8
(-0.8)
P 		84.8
(3)
P 		12.8
(-3)
P 		72
avr 28.4
(-1.9) 		32.2
(-1.6) 		22.5
(-2.3) 		46.1
(-0.7) 		99.1
(3) 		12.5
(-3) 		87
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189390_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5193
Sequence Type Exemplar sequence
Representative Public ID B0304.3 NCBI
Target Description B0304.3 /REP_DB=WormBase Gene ID /WP=CE27050 /TR=Q27465 /GB=AAK31395.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062396(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062396 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-23A1) (cyp-23A1) mRNA, complete cds. 9/11 A 
SNAP00000018301 ENSEMBL cdna:SNAP chromosome:CEL140:II:4510241:4516021:1 9/11 None
GENEFINDER00000018312 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4512497:4516240:1 9/11 None
B0304.3 ENSEMBL cdna:known chromosome:CEL140:II:4512198:4516102:1 gene:B0304.3 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4512495-4515646(+) 97.94 100.0
Public Domain and Genome References
Gene Title Cytochrome P450
Gene Symbol cyp-23A1
Ensembl B0304.3
Entrez Gene 173787 Entrez gene
SwissProt Q27465 EMBL-EBI
WormBase CE27050 Wormbase
Functional Annotations
Ortholog
ID Title Organism
MU11KSUBA:U62294_S_AT cytochrome P450, family 2, subfamily j, polypeptide 5 mm
MG-U74AV2:92814_AT cytochrome P450, family 2, subfamily j, polypeptide 5 mm
MOE430A:1417532_AT cytochrome P450, family 2, subfamily j, polypeptide 5 mm
MOUSE430A_2:1417532_AT cytochrome P450, family 2, subfamily j, polypeptide 5 mm
MOUSE430_2:1417532_AT cytochrome P450, family 2, subfamily j, polypeptide 5 mm
MOE430A:1417531_AT cytochrome P450, family 2, subfamily j, polypeptide 5 mm
MOUSE430A_2:1417531_AT cytochrome P450, family 2, subfamily j, polypeptide 5 mm
MOUSE430_2:1417531_AT cytochrome P450, family 2, subfamily j, polypeptide 5 mm
MU19KSUBA:TC24654_I_AT Cytochrome P450, family 2, subfamily j, polypeptide 5, mRNA (cDNA clone MGC:13729 IMAGE:4161995) mm
MU19KSUBC:TC38837_S_AT Cytochrome P450, family 2, subfamily j, polypeptide 5, mRNA (cDNA clone MGC:13729 IMAGE:4161995) mm
MU19KSUBC:TC40773_AT Cytochrome P450, family 2, subfamily j, polypeptide 5, mRNA (cDNA clone MGC:13729 IMAGE:4161995) mm
MU19KSUBC:TC40773_G_AT Cytochrome P450, family 2, subfamily j, polypeptide 5, mRNA (cDNA clone MGC:13729 IMAGE:4161995) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31395 Cytochrome p450 family protein 23A1 [Caenorhabditis elegans] ref|NP_494797.1| CYtochrome P450 family member (cyp-23A1) [Caenorhabditis elegans] 0.0
blast T15318 hypothetical protein B0304.3 - Caenorhabditis elegans 0.0
blast CAE67878 Hypothetical protein CBG13471 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-130
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-128
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-131
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.1E-111
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.1E-111
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.1E-111
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494797.1 3 7-24,30-47,89-111
B0304.3 3 7-24,30-47,89-111
SNAP00000018301 1 31-50
Sequence
Target Sequence 
>C. ELEGANS:189390_AT
taaccaccctgaaatgggctttccttctaatggctaaaaatcaaaaagtacagaaaaatg
ttcaagctgaactagacagtattggtcagccaatgattgaaattcaacatcgaacaaggc
ttccgtatgttcaagcaacaatcaatgaaattcaacgaattgcaaatattcttccaataa
atttattgagaactgtagcagaggatattgaaatcgatggatataatttcaaaaaaggag
atcttataattcctcaaatttcaattttgatgaatgatcctgaaatattcgaaaatccag
aagaattcaatccatcgcggtttttggatgaagataataatgtgaaaaagattgacgaat
ttctaccattttcaattggaaggaggcaatgtttaggagaatcattggctcgggcagagt
tgtacctggtttttgcaaatttaatacaaaatttcaattttgaagtggcggatgatgtga
cgactgaggtttgtttgaaacttggaaatccaaatttttcaactagaattttcaagttta
aaattttttatagttttacacgactaggtacattcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAACCACCCTGAAATGGGCTTTCCT 160 635 1034 Antisense
GAAATGGGCTTTCCTTCTAATGGCT 244 361 1044 Antisense
TGGGCTTTCCTTCTAATGGCTAAAA 365 553 1048 Antisense
GAACTAGACAGTATTGGTCAGCCAA 571 349 1102 Antisense
ATTCAACATCGAACAAGGCTTCCGT 77 13 1135 Antisense
AAGGCTTCCGTATGTTCAAGCAACA 700 159 1149 Antisense
CAACGAATTGCAAATATTCTTCCAA 698 187 1186 Antisense
GAGATCTTATAATTCCTCAAATTTC 704 395 1271 Antisense
CCAGAAGAATTCAATCCATCGCGGT 199 271 1330 Antisense
GATGTGACGACTGAGGTTTGTTTGA 166 409 1507 Antisense
GTTTTACACGACTAGGTACATTCAA 433 447 1586 Antisense
Sequence Source Affymetrix Proprietary Database