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[189386_at] cytochrome P450

Description C49C8.4 /REP_DB=WormBase Gene ID /WP=CE08839 /TR=Q27482 /GB=AAB03125.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 61      4grp_highest_8_8 > 44      4grp_high_8_8 > 16      6grp_highest_nonorm > 81      6grp_total > 61      4grp_total > 31      6grp_highest_sub > 03_04      6grp_cgc > 83      6grp_high1_sub > 04      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 269.2
(3)
P 		126.4
(-3)
P 		168
(-1.3)
P 		194.3
(-0.1)
P 		143
B 247.2
(3)
P 		144.7
(-2.8)
P 		140.7
(-3)
P 		208.3
(0.8)
P 		107
C 254
(3)
P 		197.6
(-3)
P 		211.4
(-1.5)
P 		204.1
(-2.3)
P 		56
avr 256.8
(3) 		156.2
(-3) 		173.4
(-2) 		202.2
(-0.3) 		101

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 302
1 2 3 4 5 6 range
A 208.2
(-1.4)
P 		277.5
(0.6)
P 		326.7
(2.1)
P 		358.3
(3)
P 		262.6
(0.2)
P 		155.5
(-3)
P 		203
B 277.6
(-1.3)
P 		247.5
(-2)
P 		457.4
(3)
P 		375.9
(1)
P 		240.8
(-2.2)
P 		207.7
(-3)
P 		250
C 197.4
(-2.8)
P 		258.6
(-0.7)
P 		321.8
(1.5)
P 		364.4
(3)
P 		220.9
(-2)
P 		191.7
(-3)
P 		173
avr 227.7
(-1.6) 		261.2
(-0.5) 		368.6
(3) 		366.2
(2.9) 		241.4
(-1.2) 		185
(-3) 		184
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 98
(3)
P 		27.4
(-3)
P 		43.9
(-1.6)
P 		28.4
(-2.9)
P 		71
B 78.8
(3)
P 		35.7
(-2.6)
P 		32.6
(-3)
P 		44.4
(-1.5)
P 		46
C 84.1
(3)
P 		56.8
(-0.3)
P 		73.7
(1.7)
P 		34.4
(-3)
P 		50
avr 87
(3) 		40
(-2.5) 		50.1
(-1.3) 		35.7
(-3) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 38.4
(-3)
P 		98.7
(3)
P 		71.1
(0.3)
P 		90.9
(2.2)
P 		79.2
(1.1)
P 		37.9
(-3)
P 		61
B 96.6
(0.4)
P 		82.4
(-2.1)
P 		111.1
(3)
P 		99.6
(1)
P 		97.9
(0.6)
P 		77.4
(-3)
P 		34
C 73.2
(0.1)
P 		78.9
(2)
P 		81.9
(3)
P 		73.3
(0.1)
P 		71.5
(-0.5)
P 		64.2
(-3)
P 		18
avr 69.4
(-1) 		86.7
(2.7) 		88
(3) 		87.9
(3) 		82.9
(1.9) 		59.8
(-3) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189386_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9677
Sequence Type Exemplar sequence
Representative Public ID C49C8.4 NCBI
Target Description C49C8.4 /REP_DB=WormBase Gene ID /WP=CE08839 /TR=Q27482 /GB=AAB03125.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069079(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069079 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-33E1) (cyp-33E1) mRNA, complete cds. 11/11 None
SNAP00000039871 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8645564:8648162:-1 11/11 None
GENEFINDER00000039877 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8645564:8648162:-1 11/11 None
C49C8.4 ENSEMBL cdna:known chromosome:CEL140:IV:8645554:8648163:-1 gene:C49C8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8645569-8648168(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-33E1
Ensembl C49C8.4
Entrez Gene 183602 Entrez gene
SwissProt Q27482 EMBL-EBI
WormBase CE31452 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1589244_AT similar to cytochrome P450, family 2, subfamily A, polypeptide 13 cfa
CANINE_2:CFA.13404.1.A1_AT similar to cytochrome P450, family 2, subfamily A, polypeptide 13 cfa
CANINE_2:CFA.17848.1.S1_S_AT similar to cytochrome P450, family 2, subfamily A, polypeptide 13 cfa
CANINE_2:CFAAFFX.8510.1.S1_S_AT similar to cytochrome P450, family 2, subfamily A, polypeptide 13 cfa
HG-U95AV2:1493_R_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HC-G110:1493_R_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U95AV2:1492_F_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HC-G110:1492_F_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133_PLUS_2:207244_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133A:207244_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133A_2:207244_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133_PLUS_2:211295_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133A:211295_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133A_2:211295_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133_PLUS_2:207718_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133A:207718_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-FOCUS:207718_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133A_2:207718_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U95AV2:1494_F_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HC-G110:1494_F_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133A:1494_F_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133_PLUS_2:1494_F_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-FOCUS:1494_F_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133A_2:1494_F_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HU35KSUBA:K03192_F_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HUGENEFL:K03192_F_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U95AV2:1338_S_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HC-G110:1338_S_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HUGENEFL:X13930_F_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HUGENEFL:M33318_R_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
U133_X3P:G6470138_3P_S_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
U133_X3P:G4503216_3P_S_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
U133_X3P:G13699808_3P_S_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
U133_X3P:G13699805_3P_S_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
U133_X3P:M33318MRNA_3P_S_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
U133_X3P:207244_3P_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
U133_X3P:1494_3P_F_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133A_2:214320_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133_PLUS_2:214320_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U133A:214320_X_AT cytochrome P450, family 2, subfamily A, polypeptide 6 hs
HG-U95AV2:1555_F_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
HC-G110:1555_F_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
HG-U133_PLUS_2:207718_X_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
HG-U133A:207718_X_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
HG-FOCUS:207718_X_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
HG-U133A_2:207718_X_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
HG-U95AV2:1338_S_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
HC-G110:1338_S_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
HUGENEFL:U22029_F_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
HUGENEFL:M33317_F_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
U133_X3P:G6470138_3P_S_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
U133_X3P:G4503216_3P_S_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
U133_X3P:G13699808_3P_S_AT cytochrome P450, family 2, subfamily A, polypeptide 7 hs
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB03125 Hypothetical protein C49C8.4 [Caenorhabditis elegans] ref|NP_501480.2| CYtochrome P450 family member (cyp-33E1) [Caenorhabditis elegans] 0.0
blast AAB03160 Cytochrome p450 family protein 33E2 [Caenorhabditis elegans] ref|NP_501470.1| CYtochrome P450 family member (cyp-33E2) [Caenorhabditis elegans] 0.0
blast CAE60003 Hypothetical protein CBG03499 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-140
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.4E-110
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501480.2 1 2-20
C49C8.4 1 2-20
SNAP00000039871 1 2-20
GENEFINDER00000039877 1 2-20
Sequence
Target Sequence 
>C. ELEGANS:189386_AT
gcacttctctatgtccttttgaatccagaagtacgtcaaaaagtctatgaagaattggaa
agagaaattggaagtgataggattattacaactaccgaccgacctaaactcaactatatc
aatgcaactgttaatgaatctcaacgtcttgctaatcttcttccaatgaatctatctaga
tctacaaatgctgatgttgaaattgctggctaccgtattccaaaagatactgtaatcact
ccacaaatcagttcagtaatgtatgatccagagatattccctgaaccatacgaattcaaa
cctgaaagattcctagaatcggatggaagtttgaaaaaagtagaagaacttgtgccattt
tcaattggaaaacggcaatgtcttggagaaggactggccaaaatggaactattcttgtat
tttgcaaatttgttcaacaaatttgatattaaatttcacgaatcgaatccaaatccaagt
atta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCACTTCTCTATGTCCTTTTGAATC 137 317 994 Antisense
GTCCTTTTGAATCCAGAAGTACGTC 367 475 1006 Antisense
GAATCTCAACGTCTTGCTAATCTTC 219 329 1129 Antisense
GCTAATCTTCTTCCAATGAATCTAT 196 303 1144 Antisense
GAAATTGCTGGCTACCGTATTCCAA 604 363 1192 Antisense
GATACTGTAATCACTCCACAAATCA 632 423 1219 Antisense
GATCCAGAGATATTCCCTGAACCAT 569 415 1258 Antisense
GAACCATACGAATTCAAACCTGAAA 414 345 1276 Antisense
GAAGAACTTGTGCCATTTTCAATTG 402 339 1336 Antisense
GGAACTATTCTTGTATTTTGCAAAT 515 529 1398 Antisense
GAATCGAATCCAAATCCAAGTATTA 538 329 1453 Antisense
Sequence Source Affymetrix Proprietary Database