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[189382_s_at] 14-3-3 protein

Description M117.2 /REP_DB=WormBase Gene ID /WP=CE06200 /TR=SW:P41932 /GB=CAA98138.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=14-3-3 protein
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 53      4grp_highest_8_8 > 12      4grp_high_8_8 > 30      6grp_highest_nonorm > 28      6grp_total > 54      4grp_total > 32      6grp_cgc > 91      6grp_high1_sub > 03      6grp_cgc_sub > 03      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6488
1 2 3 4 range
A 10735.9
(-3)
P 		15862.9
(2.7)
P 		15177.3
(1.9)
P 		16172.3
(3)
P 		5436
B 9684.8
(-3)
P 		13251.3
(1)
P 		15063
(3)
P 		13896.3
(1.7)
P 		5378
C 11122.2
(-3)
P 		12832.3
(-0.1)
P 		13142.8
(0.5)
P 		14628.3
(3)
P 		3506
avr 10514.3
(-3) 		13982.2
(1.7) 		14461
(2.4) 		14899
(3) 		4385

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2842
1 2 3 4 5 6 range
A 9163.4
(-1.2)
P 		8448.3
(-3)
P 		10799
(3)
P 		8516.7
(-2.8)
P 		8784.1
(-2.1)
P 		8778.5
(-2.2)
P 		2351
B 9139.4
(-3)
P 		9869.8
(-1)
P 		11283.9
(3)
P 		9313.2
(-2.5)
P 		9653.8
(-1.6)
P 		9404.2
(-2.3)
P 		2145
C 8442.1
(-3)
P 		10085.4
(3)
P 		9438.6
(0.6)
P 		9230.9
(-0.1)
P 		8777.8
(-1.8)
P 		9899.3
(2.3)
P 		1643
avr 8915
(-3) 		9467.8
(-0.9) 		10507.2
(3) 		9020.3
(-2.6) 		9071.9
(-2.4) 		9360.7
(-1.3) 		1592
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2213
1 2 3 4 range
A 3908.9
(2.8)
P 		3443.4
(1)
P 		3967.6
(3)
P 		2367.3
(-3)
P 		1600
B 3088.4
(-1.6)
P 		3272.8
(0.5)
P 		3490.4
(3)
P 		2961.3
(-3)
P 		529
C 3681.8
(0.4)
P 		3689.4
(0.5)
P 		4580.7
(3)
P 		2466.6
(-3)
P 		2114
avr 3559.7
(1.1) 		3468.5
(0.7) 		4012.9
(3) 		2598.4
(-3) 		1415

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2233
1 2 3 4 5 6 range
A 1689.2
(-3)
P 		3004.6
(3)
P 		2351.8
(0)
P 		2160
(-0.9)
P 		2650.7
(1.4)
P 		2137.3
(-1)
P 		1315
B 3180.1
(-0.1)
P 		3285.2
(0.4)
P 		2740.1
(-1.9)
P 		2466.8
(-3)
P 		3922.2
(3)
P 		3506.8
(1.3)
P 		1455
C 3129.1
(2.2)
P 		3077.3
(2)
P 		2402.3
(-0.8)
P 		1856.3
(-3)
P 		2840.4
(1.1)
P 		3313.9
(3)
P 		1458
avr 2666.1
(0.1) 		3122.4
(2.9) 		2498.1
(-0.9) 		2161
(-3) 		3137.8
(3) 		2986
(2.1) 		977
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189382_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10277
Sequence Type Exemplar sequence
Representative Public ID M117.2 NCBI
Target Description M117.2 /REP_DB=WormBase Gene ID /WP=CE06200 /TR=SW:P41932 /GB=CAA98138.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=14-3-3 protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069834(11), U05038(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069834 NCBI Caenorhabditis elegans abnormal embryonic PARtitioning of cytoplasm family member (par-5) (par-5) mRNA, complete cds. 11/11 A 
U05038 NCBI Caenorhabditis elegans Bristol N2 14-3-3 protein mRNA, complete cds. 11/11 A 
SNAP00000015384 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11820961:11821867:-1 11/11 None
GENEFINDER00000015393 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11820961:11821867:-1 11/11 None
M117.2.1 ENSEMBL cdna:known chromosome:CEL140:IV:11820476:11821894:-1 gene:M117.2 11/11 A 
M117.2.2 ENSEMBL cdna:known chromosome:CEL140:IV:11820476:11821887:-1 gene:M117.2 11/11 A 
M117.2.4 ENSEMBL cdna:known chromosome:CEL140:IV:11820501:11821894:-1 gene:M117.2 11/11 None
M117.2.3 ENSEMBL cdna:known chromosome:CEL140:IV:11820501:11821894:-1 gene:M117.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11820966-11821873(-) 100.0 100.0
Public Domain and Genome References
Gene Title 14-3-3 protein
Gene Symbol par-5
Ensembl M117.2
Entrez Gene 178113 Entrez gene
SwissProt P41932 EMBL-EBI
WormBase CE06200 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1591859_AT similar to 14-3-3 protein beta/alpha (Protein kinase C inhibitor protein-1) (KCIP-1) cfa
CANINE_2:CFA.4344.1.S1_AT similar to 14-3-3 protein beta/alpha (Protein kinase C inhibitor protein-1) (KCIP-1) cfa
CANINE_2:CFAAFFX.14973.1.S1_S_AT similar to 14-3-3 protein beta/alpha (Protein kinase C inhibitor protein-1) (KCIP-1) cfa
CHICKEN:GGA.4816.1.S1_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide gga
HG-U133_PLUS_2:208743_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HG-U133A:208743_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HG-U133A_2:208743_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HG-FOCUS:208743_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HG-U95AV2:32324_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HG-U133A:217718_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HG-U133_PLUS_2:217718_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HG-U133A_2:217718_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HU35KSUBD:RC_W80790_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HU35KSUBD:RC_AA442767_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HUGENEFL:X57346_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
U133_X3P:G7661715_3P_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
U133_X3P:G12655022_3P_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
U133_X3P:G12655022_3P_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
U133_X3P:HS.182238.0.A1_3P_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HG-U133A_2:217717_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HG-U133_PLUS_2:217717_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
HG-U133A:217717_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide hs
MOUSE430_2:1438708_X_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOE430A:1438708_X_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOUSE430A_2:1438708_X_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MU11KSUBB:MSA.37497.0_F_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MU11KSUBB:MSA.11382.0_I_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MU11KSUBB:MSA.11382.0_F_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MU11KSUBB:MSA.10201.0_F_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOE430A:1420878_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOUSE430A_2:1420878_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOUSE430_2:1420878_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOE430A:1436783_X_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOUSE430A_2:1436783_X_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOUSE430_2:1436783_X_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOE430A:1455815_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOUSE430A_2:1455815_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOUSE430_2:1455815_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOE430A:1420879_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOUSE430A_2:1420879_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOUSE430_2:1420879_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOE430A:1420880_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOUSE430A_2:1420880_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MOUSE430_2:1420880_A_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MG-U74AV2:98053_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide mm
MU19KSUBA:TC18424_AT Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (Ywhab), mRNA mm
MU19KSUBA:TC18425_AT Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (Ywhab), mRNA mm
MU19KSUBA:TC18425_G_AT Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (Ywhab), mRNA mm
RG-U34A:S55223_S_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide rn
RAE230A:1386999_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide rn
RAT230_2:1386999_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide rn
RAE230A:1398800_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide rn
RAT230_2:1398800_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide rn
RG-U34A:RC_AA924084_AT tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
19904 protein domain specific binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98138 Hypothetical protein M117.2 [Caenorhabditis elegans] ref|NP_502235.1| abnormal embryonic PARtitioning of cytoplasm family member (par-5) [Caenorhabditis elegans] 1.0E-135
blast P41932 14-3-3-like protein 1 gb|AAA61872.1| 14-3-3 protein 1.0E-135
Protein Domains
Database ID Description E-Value
Pfam IPR000308 EMBL-EBI 14-3-3 protein 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189382_S_AT
acgaagagcgtaaccttctctctgttgcctacaaaaacgtcgtcggagctcgccgttcat
cgtggagagttatctcttccattgagcaaaagaccgagggatccgagaagaagcaacaac
tcgccaaggaataccgcgtcaaggttgagcaagaactcaacgacatctgccaagatgtgc
tcaagctcctcgacgagttcctcattgtcaaggctggagctgctgagtcgaaggtcttct
atctcaagatgaaaggagattactacagatacctcgccgaggtcgcttcagaggatcgcg
ctgccgttgttgagaagtcccagaaggcttaccaggaggctcttgatatcgctaaggaca
agatgcagccaacccacccaattcgccttggacttgccctcaacttctctgtcttctact
acgagatcttgaacactccagagcacgcttgccaactcgcgaaacaggctttcgatgatg
ctattgctgagctcgacacgttgaacgaggattcgtacaaggactcgaccttgatcatgc
aacttctacgtgacaatctcactctctggacatctgacgttggagctga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGAAGAGCGTAACCTTCTCTCTGT 52 93 131 Antisense
CCTTCTCTCTGTTGCCTACAAAAAC 237 269 144 Antisense
GCTCGCCGTTCATCGTGGAGAGTTA 679 297 178 Antisense
GCAACAACTCGCCAAGGAATACCGC 458 319 243 Antisense
ATCTGCCAAGATGTGCTCAAGCTCC 284 33 295 Antisense
TCCTCGACGAGTTCCTCATTGTCAA 27 593 317 Antisense
GAGCTGCTGAGTCGAAGGTCTTCTA 529 387 347 Antisense
ATTACTACAGATACCTCGCCGAGGT 183 13 389 Antisense
CGCCGAGGTCGCTTCAGAGGATCGC 460 257 405 Antisense
CGCTGCCGTTGTTGAGAAGTCCCAG 100 255 429 Antisense
TCTGGACATCTGACGTTGGAGCTGA 124 607 695 Antisense
Sequence Source Affymetrix Proprietary Database