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[189378_at] cytochrome p450

Description K09A11.4 /REP_DB=WormBase Gene ID /WP=CE03475 /TR=Q27507 /GB=CAA90617.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=cytochrome p450
Cluster 6grp_total > 44      4grp_total > 13      6grp_cgc > 90      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 75.8
(0.1)
P 		40.6
(-3)
A 		52.4
(-2)
A 		109
(3)
P 		68
B 62.1
(0.1)
P 		40.8
(-2.8)
A 		39.2
(-3)
M 		83.4
(3)
A 		44
C 76.9
(1.9)
P 		73.4
(1.6)
A 		29.1
(-3)
A 		87.5
(3)
P 		58
avr 71.6
(0.5) 		51.6
(-1.7) 		40.2
(-3) 		93.3
(3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 62.9
(0.9)
A 		39.1
(-2.4)
A 		77.9
(3)
P 		34.9
(-3)
A 		60.5
(0.6)
A 		35
(-3)
A 		43
B 42
(-0.1)
A 		68.8
(2.7)
P 		40.5
(-0.3)
A 		13.8
(-3)
A 		72.1
(3)
P 		44.4
(0.1)
P 		58
C 54.9
(1.3)
P 		54.9
(1.3)
A 		72.5
(3)
A 		10.7
(-3)
A 		50.5
(0.9)
A 		43.1
(0.1)
A 		62
avr 53.3
(1.6) 		54.3
(1.7) 		63.6
(3) 		19.8
(-3) 		61
(2.6) 		40.8
(-0.1) 		44
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 27.6
(3)
P 		8.8
(-3)
A 		13.7
(-1.4)
A 		16
(-0.7)
P 		19
B 19.8
(3)
P 		10.1
(-2.4)
A 		9.1
(-3)
M 		17.8
(1.9)
A 		11
C 25.5
(3)
P 		21.1
(1.3)
A 		10.2
(-3)
A 		14.7
(-1.2)
P 		15
avr 24.3
(3) 		13.3
(-2) 		11
(-3) 		16.2
(-0.7) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 11.6
(-1.1)
A 		13.9
(0.3)
A 		17
(2.2)
P 		8.9
(-2.8)
A 		18.3
(3)
A 		8.5
(-3)
A 		10
B 14.6
(-0.4)
A 		22.9
(1.5)
P 		9.8
(-1.6)
A 		3.6
(-3)
A 		29.3
(3)
P 		16.5
(0)
P 		26
C 20.4
(3)
P 		16.8
(1.8)
A 		18.5
(2.4)
A 		2.2
(-3)
A 		16.3
(1.6)
A 		14.4
(1)
A 		18
avr 15.5
(0.9) 		17.9
(1.8) 		15.1
(0.7) 		4.9
(-3) 		21.3
(3) 		13.1
(0) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189378_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17741
Sequence Type Exemplar sequence
Representative Public ID K09A11.4 NCBI
Target Description K09A11.4 /REP_DB=WormBase Gene ID /WP=CE03475 /TR=Q27507 /GB=CAA90617.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=cytochrome p450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077804(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077804 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-14A3) (cyp-14A3) mRNA, complete cds. 11/11 None
SNAP00000010028 ENSEMBL cdna:SNAP chromosome:CEL140:X:13271797:13278896:-1 11/11 None
K09A11.4 ENSEMBL cdna:known chromosome:CEL140:X:13271797:13274118:-1 gene:K09A11.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000010030 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13271796-13274118(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome p450
Gene Symbol cyp-14A3
Ensembl K09A11.4
Entrez Gene 187185 Entrez gene
SwissProt Q27507 EMBL-EBI
WormBase CE03475 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.13499.1.S1_AT similar to cytochrome P450, family 2, subfamily R, polypeptide 1 cfa
CHICKEN:GGA.14394.1.S1_AT cytochrome P450, family 2, subfamily R, polypeptide 1 gga
HG-U133_PLUS_2:207786_AT cytochrome P450, family 2, subfamily R, polypeptide 1 hs
HG-U133A:207786_AT cytochrome P450, family 2, subfamily R, polypeptide 1 hs
HG-U133A_2:207786_AT cytochrome P450, family 2, subfamily R, polypeptide 1 hs
HU35KSUBA:RC_AA449325_AT cytochrome P450, family 2, subfamily R, polypeptide 1 hs
HU35KSUBD:RC_AA235247_S_AT cytochrome P450, family 2, subfamily R, polypeptide 1 hs
U133_X3P:HS.16846.0.A1_3P_AT cytochrome P450, family 2, subfamily R, polypeptide 1 hs
U133_X3P:207786_3P_AT cytochrome P450, family 2, subfamily R, polypeptide 1 hs
HG-U133_PLUS_2:227109_AT cytochrome P450, family 2, subfamily R, polypeptide 1 hs
HG-U133B:227109_AT cytochrome P450, family 2, subfamily R, polypeptide 1 hs
HG-U95B:58365_AT cytochrome P450, family 2, subfamily R, polypeptide 1 hs
U133_X3P:G13375649_3P_X_AT Cytochrome P450, family 2, subfamily R, polypeptide 1 hs
U133_X3P:G13375649_3P_AT Cytochrome P450, family 2, subfamily R, polypeptide 1 hs
HG-U133_PLUS_2:244757_AT Cytochrome P450, family 2, subfamily R, polypeptide 1 hs
HG-U133B:244757_AT Cytochrome P450, family 2, subfamily R, polypeptide 1 hs
HG-U95E:86534_AT Cytochrome P450, family 2, subfamily R, polypeptide 1 hs
U133_X3P:HS.265459.0.A1_3P_AT Cytochrome P450, family 2, subfamily R, polypeptide 1 hs
MOE430B:1444138_AT cytochrome P450, family 2, subfamily r, polypeptide 1 mm
MOUSE430_2:1444138_AT cytochrome P450, family 2, subfamily r, polypeptide 1 mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90617 Hypothetical protein K09A11.4 [Caenorhabditis elegans] ref|NP_510205.1| CYtochrome P450 family member (cyp-14A3) [Caenorhabditis elegans] 0.0
blast CAA90615 Hypothetical protein K09A11.3 [Caenorhabditis elegans] ref|NP_510204.1| CYtochrome P450 family member (cyp-14A2) [Caenorhabditis elegans] 0.0
blast AAB04873 Cytochrome p450 protein 14A5 [Caenorhabditis elegans] ref|NP_504435.1| CYtochrome P450 family member (cyp-14A5) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-145
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-153
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.3E-120
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.6E-108
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510205.1 1 2-21
K09A11.4 1 2-21
Sequence
Target Sequence 
>C. ELEGANS:189378_AT
gcatctgttcttgatttctggcttgctggaatggaaacaacctctaactcacttcgttgg
catttggcagcaatgatgaagtatcctgaaattcaagataaagtccggaaagaaatcttt
gacaatgtaggaaccgctagattaccgtcaatgtcagacaaaccaaatatgccgtacaca
caagctgtcatacatgaagttcaaagattttcaaatatgattccaattttgggaactcac
acaaacaaagaagatatcctgcttaaagggaaaaatgtacctacaggcactgtaatattt
gctcaaatctggtcggttctcaaaaatgactcagtatttgaagatagtcacaagttcaat
ccagatagatatctcctcactgatggaaaaactttcgacaaaactattttggaaagaaca
atcccattcagtgtcggcaaacgaaattgcgttggtgaaggattggctcgaatggagctt
ttcttgattttcacagcactaattcagaagtatgaatttattcctaacggcagcattgat
ttgtctccagtgcctggtgcggttctgacaacaaagccatacacgtgccgactaattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCATCTGTTCTTGATTTCTGGCTTG 399 309 880 Antisense
ACAACCTCTAACTCACTTCGTTGGC 125 113 916 Antisense
GAACCGCTAGATTACCGTCAATGTC 286 347 1010 Antisense
ATGCCGTACACACAAGCTGTCATAC 651 39 1048 Antisense
TTTGCTCAAATCTGGTCGGTTCTCA 490 665 1177 Antisense
GATAGATATCTCCTCACTGATGGAA 659 423 1243 Antisense
AATCCCATTCAGTGTCGGCAAACGA 634 165 1299 Antisense
ATGGAGCTTTTCTTGATTTTCACAG 179 53 1351 Antisense
ACGGCAGCATTGATTTGTCTCCAGT 278 97 1406 Antisense
GGTGCGGTTCTGACAACAAAGCCAT 79 501 1435 Antisense
AAGCCATACACGTGCCGACTAATTC 358 147 1453 Antisense
Sequence Source Affymetrix Proprietary Database