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[189374_s_at] Yeast ORF YKL162

Description F45H7.6 /REP_DB=WormBase Gene ID /WP=CE01588 /TR=Q09547 /GB=CAA84325.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Yeast ORF YKL162
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      4grp_highest_8_8 > 56      4grp_high_8_8 > 34      6grp_highest_nonorm > 70      6grp_total > 25      4grp_total > 37      6grp_highest_sub > 01_02      6grp_high1_sub > 01_02      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 241
1 2 3 4 range
A 433.9
(3)
P 		353.5
(0.6)
P 		233.8
(-3)
P 		355.3
(0.6)
P 		200
B 401.2
(3)
P 		377.2
(1.8)
P 		279.5
(-3)
P 		360.6
(1)
P 		122
C 474.6
(3)
P 		428.7
(1.8)
P 		296.8
(-1.7)
P 		245.8
(-3)
P 		229
avr 436.6
(3) 		386.5
(1.2) 		270
(-3) 		320.6
(-1.2) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 399
(3)
P 		300.7
(0.2)
P 		245.4
(-1.3)
P 		276.9
(-0.4)
P 		236.1
(-1.6)
P 		186
(-3)
P 		213
B 392
(2.8)
P 		397.9
(3)
P 		313.5
(0.8)
P 		303.6
(0.5)
P 		172.1
(-3)
P 		276.8
(-0.2)
P 		226
C 356
(1.2)
P 		417.1
(3)
P 		248
(-2)
P 		219.3
(-2.9)
P 		215
(-3)
P 		286.4
(-0.9)
P 		202
avr 382.3
(3) 		371.9
(2.6) 		269
(-0.9) 		266.6
(-1) 		207.7
(-3) 		249.7
(-1.6) 		175
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 158
(3)
P 		76.7
(-1.6)
P 		61.1
(-2.5)
P 		52
(-3)
P 		106
B 127.9
(3)
P 		93.2
(-0.3)
P 		64.8
(-3)
P 		76.8
(-1.9)
P 		63
C 157.1
(3)
P 		123.3
(1.2)
P 		103.4
(0.2)
P 		41.4
(-3)
P 		116
avr 147.7
(3) 		97.7
(-0.3) 		76.4
(-1.7) 		56.7
(-3) 		91

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 73.6
(-0.2)
P 		106.9
(3)
P 		53.4
(-2.2)
P 		70.2
(-0.6)
P 		71.3
(-0.5)
P 		45.3
(-3)
P 		62
B 136.4
(3)
P 		132.4
(2.6)
P 		76.1
(-2.4)
P 		80.4
(-2.1)
P 		69.9
(-3)
P 		103.2
(0)
P 		67
C 131.9
(3)
P 		127.3
(2.7)
P 		63.1
(-1.7)
P 		44.1
(-3)
P 		69.6
(-1.3)
P 		95.9
(0.5)
P 		88
avr 114
(2.2) 		122.2
(3) 		64.2
(-3) 		64.9
(-2.9) 		70.3
(-2.4) 		81.5
(-1.2) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189374_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6300
Sequence Type Exemplar sequence
Representative Public ID F45H7.6 NCBI
Target Description F45H7.6 /REP_DB=WormBase Gene ID /WP=CE01588 /TR=Q09547 /GB=CAA84325.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Yeast ORF YKL162
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065296(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065296 NCBI Caenorhabditis elegans F45H7.6 (F45H7.6) mRNA, complete cds. 11/11 A 
F45H7.6 ENSEMBL cdna:known chromosome:CEL140:III:3377397:3384157:-1 gene:F45H7.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3377422-3384157(-) 100.0 100.0
Public Domain and Genome References
Gene Title Yeast ORF YKL162
Ensembl F45H7.6
Entrez Gene 175438 Entrez gene
SwissProt Q09547 EMBL-EBI
WormBase CE01588 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1590261_AT similar to NEDD4-like ubiquitin-protein ligase 1 cfa
CANINE_2:CFA.8453.1.A1_AT similar to NEDD4-like ubiquitin-protein ligase 1 cfa
CANINE_2:CFAAFFX.6189.1.S1_S_AT similar to NEDD4-like ubiquitin-protein ligase 1 cfa
CHICKEN:GGAAFFX.20797.1.S1_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 gga
CHICKEN:GGAAFFX.7819.1.S1_S_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 gga
CHICKEN:GGAAFFX.7819.3.S1_S_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 gga
HG-U133_PLUS_2:210331_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133A:210331_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133A_2:210331_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133_PLUS_2:215584_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133A_2:215584_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133A:215584_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HU35KSUBA:AB002320_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
U133_X3P:G10039442_3P_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
U133_X3P:HS.287538.0.S1_3P_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U95AV2:35011_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
U133_X3P:HS2.13310.1.S1_3P_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133_PLUS_2:1569659_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133_PLUS_2:1563480_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133_PLUS_2:243743_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133B:243743_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U95B:42187_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U95C:65347_R_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U95E:88999_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HU35KSUBB:RC_R60040_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HU35KSUBD:RC_AA432127_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
U133_X3P:HS.13310.0.A1_3P_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
U133_X3P:HS.164453.0.A1_3P_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
MOUSE430_2:1460030_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOE430B:1460030_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MG-U74CV2:136261_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOE430B:1456527_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOUSE430_2:1456527_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOE430B:1441101_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOUSE430_2:1441101_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOE430B:1440656_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOUSE430_2:1440656_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
RAE230B:1393622_AT similar to HECT type E3 ubiquitin ligase rn
RAT230_2:1393622_AT similar to HECT type E3 ubiquitin ligase rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4842 ubiquitin-protein ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86773 Hypothetical protein F45H7.6 [Caenorhabditis elegans] emb|CAA84325.1| Hypothetical protein F45H7.6 [Caenorhabditis elegans] ref|NP_497697.1| F45H7.6 [Caenorhabditis elegans] 0.0
blast CAE65019 Hypothetical protein CBG09857 [Caenorhabditis briggsae] 0.0
blast XP_392900 PREDICTED: similar to HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 [Apis mellifera] 2.0E-97
Protein Domains
Database ID Description E-Value
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 1.2E-10
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 9.1E-8
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 1.6E-74
Sequence
Target Sequence 
>C. ELEGANS:189374_S_AT
ctctgattgtcatatagtcgtcgaatggttttgggaagtgatagagacgatgaccaacca
agaaagatttgatttgttgttgtttgtcaccggcagttcatcagttccttttgaaggatt
ctctgcgctacgtggcaatgaggaaataagcaagttttgcatagagaagtggggagatgc
cacgtcgtttcccagggctcatacgtgtttcaacaggcttcaactaccctcctataacac
taagcagcagttgaaatcaaaacttcagcaggcaatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCTGATTGTCATATAGTCGTCGAA 113 233 2376 Antisense
GTCGTCGAATGGTTTTGGGAAGTGA 635 471 2392 Antisense
GTGATAGAGACGATGACCAACCAAG 234 483 2413 Antisense
ATTTGTTGTTGTTTGTCACCGGCAG 693 17 2447 Antisense
GGCAGTTCATCAGTTCCTTTTGAAG 234 535 2467 Antisense
AGTGGGGAGATGCCACGTCGTTTCC 280 59 2543 Antisense
TCGTTTCCCAGGGCTCATACGTGTT 127 603 2560 Antisense
AGGGCTCATACGTGTTTCAACAGGC 54 59 2569 Antisense
GTTTCAACAGGCTTCAACTACCCTC 25 449 2582 Antisense
AACTACCCTCCTATAACACTAAGCA 322 109 2597 Antisense
GAAATCAAAACTTCAGCAGGCAATT 393 363 2628 Antisense
Sequence Source Affymetrix Proprietary Database