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[189373_at] Yeast ORF YKL162

Description F45H7.6 /REP_DB=WormBase Gene ID /WP=CE01588 /TR=Q09547 /GB=CAA84325.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Yeast ORF YKL162
Cluster 6grp_total > 18      4grp_total > 38      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 21
(-3)
A 		44
(3)
A 		26.2
(-1.6)
A 		42.5
(2.6)
A 		23
B 44
(3)
P 		14.7
(-3)
A 		23.4
(-1.2)
A 		30
(0.1)
A 		29
C 62.3
(3)
A 		34.7
(-1.2)
A 		36.9
(-0.9)
A 		22.9
(-3)
A 		39
avr 42.4
(3) 		31.1
(-2) 		28.8
(-3) 		31.8
(-1.7) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 55.4
(3)
A 		53.6
(2.7)
A 		47.3
(1.8)
A 		24.3
(-1.7)
A 		38.8
(0.5)
A 		16
(-3)
A 		39
B 47.3
(2)
A 		22.5
(-2.2)
A 		53.1
(3)
A 		19.2
(-2.8)
A 		33
(-0.4)
A 		18
(-3)
A 		35
C 71.2
(3)
A 		29.4
(-1.1)
M 		10.2
(-3)
A 		59.5
(1.8)
A 		41.1
(0)
A 		42.5
(0.2)
A 		61
avr 58
(3) 		35.2
(-1.2) 		36.9
(-0.9) 		34.3
(-1.4) 		37.6
(-0.8) 		25.5
(-3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 7.7
(-0.4)
A 		9.6
(3)
A 		6.8
(-1.9)
A 		6.2
(-3)
A 		3
B 14
(3)
P 		3.6
(-3)
A 		5.4
(-2)
A 		6.4
(-1.4)
A 		10
C 20.6
(3)
A 		10
(-0.8)
A 		12.9
(0.2)
A 		3.9
(-3)
A 		17
avr 14.1
(3) 		7.7
(-1.5) 		8.4
(-1) 		5.5
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 10.2
(-0.5)
A 		19
(3)
A 		10.3
(-0.5)
A 		6.2
(-2.1)
A 		11.7
(0.1)
A 		3.9
(-3)
A 		15
B 16.5
(3)
A 		7.5
(-1.7)
A 		12.9
(1.1)
A 		5.1
(-3)
A 		13.4
(1.4)
A 		6.7
(-2.2)
A 		11
C 26.4
(3)
A 		9
(-1.4)
M 		2.6
(-3)
A 		12
(-0.6)
A 		13.3
(-0.3)
A 		14.2
(-0.1)
A 		24
avr 17.7
(3) 		11.8
(-0.6) 		8.6
(-2.5) 		7.8
(-3) 		12.8
(0) 		8.3
(-2.7) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189373_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6300
Sequence Type Exemplar sequence
Representative Public ID F45H7.6 NCBI
Target Description F45H7.6 /REP_DB=WormBase Gene ID /WP=CE01588 /TR=Q09547 /GB=CAA84325.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Yeast ORF YKL162
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065296(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065296 NCBI Caenorhabditis elegans F45H7.6 (F45H7.6) mRNA, complete cds. 11/11 A 
F45H7.6 ENSEMBL cdna:known chromosome:CEL140:III:3377397:3384157:-1 gene:F45H7.6 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000009573 3/11 Cross Hyb Matching Probes None
GENEFINDER00000009580 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3377422-3384157(-) 100.0 100.0
Public Domain and Genome References
Gene Title Yeast ORF YKL162
Ensembl F45H7.6
Entrez Gene 175438 Entrez gene
SwissProt Q09547 EMBL-EBI
WormBase CE01588 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1590261_AT similar to NEDD4-like ubiquitin-protein ligase 1 cfa
CANINE_2:CFA.8453.1.A1_AT similar to NEDD4-like ubiquitin-protein ligase 1 cfa
CANINE_2:CFAAFFX.6189.1.S1_S_AT similar to NEDD4-like ubiquitin-protein ligase 1 cfa
CHICKEN:GGAAFFX.20797.1.S1_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 gga
CHICKEN:GGAAFFX.7819.1.S1_S_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 gga
CHICKEN:GGAAFFX.7819.3.S1_S_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 gga
HG-U133_PLUS_2:210331_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133A:210331_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133A_2:210331_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133_PLUS_2:215584_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133A_2:215584_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133A:215584_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HU35KSUBA:AB002320_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
U133_X3P:G10039442_3P_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
U133_X3P:HS.287538.0.S1_3P_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U95AV2:35011_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
U133_X3P:HS2.13310.1.S1_3P_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133_PLUS_2:1569659_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133_PLUS_2:1563480_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133_PLUS_2:243743_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U133B:243743_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U95B:42187_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U95C:65347_R_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HG-U95E:88999_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HU35KSUBB:RC_R60040_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
HU35KSUBD:RC_AA432127_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
U133_X3P:HS.13310.0.A1_3P_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
U133_X3P:HS.164453.0.A1_3P_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 hs
MOUSE430_2:1460030_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOE430B:1460030_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MG-U74CV2:136261_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOE430B:1456527_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOUSE430_2:1456527_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOE430B:1441101_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOUSE430_2:1441101_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOE430B:1440656_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
MOUSE430_2:1440656_AT HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 mm
RAE230B:1393622_AT similar to HECT type E3 ubiquitin ligase rn
RAT230_2:1393622_AT similar to HECT type E3 ubiquitin ligase rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4842 ubiquitin-protein ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86773 Hypothetical protein F45H7.6 [Caenorhabditis elegans] emb|CAA84325.1| Hypothetical protein F45H7.6 [Caenorhabditis elegans] ref|NP_497697.1| F45H7.6 [Caenorhabditis elegans] 0.0
blast CAE65019 Hypothetical protein CBG09857 [Caenorhabditis briggsae] 0.0
blast XP_392900 PREDICTED: similar to HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 [Apis mellifera] 2.0E-97
Protein Domains
Database ID Description E-Value
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 1.2E-10
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 9.1E-8
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 1.6E-74
Sequence
Target Sequence 
>C. ELEGANS:189373_AT
gtgagtgaccttaccttatcatctattgtttccgtagcattacagctagccgaacaagaa
cttgtacccggtggagaatcacaaatggtcactgaagctaataaagcagaatttatcgat
ttgatgtgtcagaaaaaggcgataagaggtgtggagaagccattggagattctattgaca
tcttttaatcagatattgaatgataatctcttaaactcactggaaagttcggatttgaag
cggattcttagtggatcactg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGAGTGACCTTACCTTATCATCTA 242 483 2065 Antisense
CCTTATCATCTATTGTTTCCGTAGC 528 269 2078 Antisense
GTTTCCGTAGCATTACAGCTAGCCG 502 451 2092 Antisense
ACAGCTAGCCGAACAAGAACTTGTA 566 107 2106 Antisense
GAACTTGTACCCGGTGGAGAATCAC 547 347 2122 Antisense
GAATCACAAATGGTCACTGAAGCTA 350 327 2140 Antisense
GAAGCCATTGGAGATTCTATTGACA 636 343 2220 Antisense
ATTCTATTGACATCTTTTAATCAGA 283 11 2233 Antisense
GATAATCTCTTAAACTCACTGGAAA 253 423 2266 Antisense
AGTTCGGATTTGAAGCGGATTCTTA 104 65 2290 Antisense
GAAGCGGATTCTTAGTGGATCACTG 578 343 2301 Antisense
Sequence Source Affymetrix Proprietary Database