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[189351_at]

Description F53A2.8 /REP_DB=WormBase Gene ID /WP=CE16100 /TR=O45553 /GB=CAB04456.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=FYVE zinc finger
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 49      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 81      6grp_total > 38      4grp_total > 47      6grp_highest_sub > Cu      6grp_cgc > 09      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 304
1 2 3 4 range
A 462.1
(3)
P 		242.9
(-2.3)
P 		215.6
(-2.9)
P 		212.6
(-3)
P 		250
B 400.4
(3)
P 		225.5
(-1.3)
P 		157.7
(-3)
P 		236.5
(-1.1)
P 		243
C 366.3
(3)
P 		198.4
(-3)
P 		232.5
(-1.8)
P 		205.5
(-2.7)
P 		168
avr 409.6
(3) 		222.3
(-2.4) 		201.9
(-3) 		218.2
(-2.5) 		208

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 195
1 2 3 4 5 6 range
A 317.5
(1)
P 		322.5
(1.2)
P 		226.3
(-3)
P 		251.7
(-1.9)
P 		251.6
(-1.9)
P 		363.4
(3)
P 		137
B 314.6
(2.4)
P 		329.7
(3)
P 		190.2
(-3)
P 		301.7
(1.8)
P 		280.9
(0.9)
P 		310.5
(2.2)
P 		140
C 385.3
(3)
P 		295.5
(-0.8)
P 		241.7
(-3)
P 		306.6
(-0.3)
P 		309.6
(-0.2)
P 		246.6
(-2.8)
P 		144
avr 339.1
(3) 		315.9
(1.8) 		219.4
(-3) 		286.7
(0.4) 		280.7
(0.1) 		306.8
(1.4) 		120
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 168.3
(3)
P 		52.7
(-2.1)
P 		56.4
(-1.9)
P 		31.1
(-3)
P 		137
B 127.7
(3)
P 		55.7
(-1.7)
P 		36.5
(-3)
P 		50.4
(-2.1)
P 		91
C 121.2
(3)
P 		57
(-1.5)
P 		81
(0.2)
P 		34.7
(-3)
P 		87
avr 139.1
(3) 		55.1
(-2) 		58
(-1.8) 		38.7
(-3) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 97
1 2 3 4 5 6 range
A 58.5
(-2.2)
P 		114.7
(3)
P 		49.3
(-3)
P 		63.8
(-1.7)
P 		75.9
(-0.6)
P 		88.5
(0.6)
P 		65
B 109.5
(2.5)
P 		109.7
(2.5)
P 		46.2
(-3)
P 		79.9
(-0.1)
P 		114.1
(2.9)
P 		115.8
(3)
P 		70
C 142.8
(3)
P 		90.2
(-0.9)
P 		61.5
(-3)
P 		61.7
(-3)
P 		100.2
(-0.1)
P 		82.5
(-1.5)
P 		81
avr 103.6
(2.9) 		104.9
(3) 		52.3
(-3) 		68.5
(-1.2) 		96.7
(2.1) 		95.6
(1.9) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189351_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7713
Sequence Type Exemplar sequence
Representative Public ID F53A2.8 NCBI
Target Description F53A2.8 /REP_DB=WormBase Gene ID /WP=CE16100 /TR=O45553 /GB=CAB04456.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=FYVE zinc finger
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067352(11), NM_001027431(11), NM_181951(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067352 NCBI Caenorhabditis elegans MTM (myotubularin) family member (mtm-6) (mtm-6) mRNA, complete cds. 11/11 None
NM_001027431 NCBI Caenorhabditis elegans MTM (myotubularin) family member (mtm-6) (mtm-6) mRNA, complete cds. 11/11 None
NM_181951 NCBI Caenorhabditis elegans MTM (myotubularin) family member (mtm-6) (mtm-6) mRNA, complete cds. 11/11 None
SNAP00000020112 ENSEMBL cdna:SNAP chromosome:CEL140:III:13350830:13354976:1 11/11 None
GENEFINDER00000020117 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13350926:13354976:1 11/11 None
F53A2.8a ENSEMBL cdna:known chromosome:CEL140:III:13346601:13354976:1 gene:F53A2.8 11/11 None
F53A2.8c ENSEMBL cdna:known chromosome:CEL140:III:13346601:13355191:1 gene:F53A2.8 11/11 None
F53A2.8b.1 ENSEMBL cdna:known chromosome:CEL140:III:13350842:13354976:1 gene:F53A2.8 11/11 None
F53A2.8b.2 ENSEMBL cdna:known chromosome:CEL140:III:13350909:13354976:1 gene:F53A2.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13346616-13354972(+) 100.0 100.0
Public Domain and Genome References
Ensembl F53A2.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04456 Hypothetical protein F53A2.8a [Caenorhabditis elegans] ref|NP_499753.1| MTM (myotubularin) family member (mtm-6) [Caenorhabditis elegans] gb|AAP79302.1| myotubularin-related protein MTM-6A [Caenorhabditis elegans] 0.0
blast CAH10812 Hypothetical protein F53A2.8c [Caenorhabditis elegans] ref|NP_001022602.1| MTM (myotubularin) family member (mtm-6) [Caenorhabditis elegans] 0.0
blast CAD45596 Hypothetical protein F53A2.8b [Caenorhabditis elegans] ref|NP_871680.1| MTM (myotubularin) family member (mtm-6) [Caenorhabditis elegans] gb|AAP79304.1| myotubularin-related protein MTM-6B [Caenorhabditis elegans] 0.0
blast CAE56892 Hypothetical protein CBG24732 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR010569 EMBL-EBI Myotubularin-related 3.8E-65
Pfam IPR000306 EMBL-EBI Zn-finger, FYVE type 4.1E-7
Pfam IPR010569 EMBL-EBI Myotubularin-related 3.8E-65
Pfam IPR000306 EMBL-EBI Zn-finger, FYVE type 4.1E-7
Pfam IPR010569 EMBL-EBI Myotubularin-related 3.8E-65
Pfam IPR000306 EMBL-EBI Zn-finger, FYVE type 4.1E-7
Pfam IPR010569 EMBL-EBI Myotubularin-related 3.8E-65
Pfam IPR000306 EMBL-EBI Zn-finger, FYVE type 4.1E-7
Sequence
Target Sequence 
>C. ELEGANS:189351_AT
atgaatccattttattcgcctaccgcacacgttgctctactggatcttgacactcgtgca
gctcgcttcacagtttggactgcaatgtataatcgattcgacaatggtctacaaccaaga
gaacggcttgaagacctaactatggctgcgatggagcacgttggagtactagaatcccac
gtggcacagttgagaacgagactagccgaactgaagacacaacaaaatcaacagataact
tcaacgaatacaccgacgaatatggttgatagtggaatgagcagtgcaactgatgatttg
aagaatctcagtttaagccatcctttggatccattgagctctacactaccgattctggaa
agagcaacaagtcaagaaagtggagtcatggattcatcgttatactatcctgatgaggct
ctgaccaaatattcactaaaatggcaacctcttcgtggagccgatagatgctcaaatcca
gcgtgccgaggggagttctcatcgacgattgagagaagaattcattgtcatttgtgtgga
atgattttctgccgccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGAATCCATTTTATTCGCCTACCG 180 45 1426 Antisense
CAGCTCGCTTCACAGTTTGGACTGC 59 203 1484 Antisense
GAAGACCTAACTATGGCTGCGATGG 414 339 1555 Antisense
GGAGTACTAGAATCCCACGTGGCAC 49 505 1588 Antisense
AGAATCTCAGTTTAAGCCATCCTTT 96 75 1727 Antisense
CCTTTGGATCCATTGAGCTCTACAC 62 269 1747 Antisense
AGCTCTACACTACCGATTCTGGAAA 470 79 1762 Antisense
ACTATCCTGATGAGGCTCTGACCAA 508 101 1829 Antisense
AAAATGGCAACCTCTTCGTGGAGCC 513 127 1863 Antisense
TGCCGAGGGGAGTTCTCATCGACGA 17 585 1909 Antisense
TGTGTGGAATGATTTTCTGCCGCCG 679 555 1958 Antisense
Sequence Source Affymetrix Proprietary Database