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[189347_at] tRNA synthetase

Description T07A9.2 /REP_DB=WormBase Gene ID /WP=CE17211 /TR=O44413 /GB=AAK39275.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=tRNA synthetase
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 53      4grp_highest_8_8 > 60      4grp_high_8_8 > 25      6grp_highest_nonorm > 62      6grp_total > 30      4grp_total > 38      6grp_highest_sub > 03      6grp_cgc > 72      6grp_high1_sub > 03      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 208
1 2 3 4 range
A 539.5
(3)
P 		366.1
(-2.1)
P 		337.4
(-3)
P 		369.4
(-2)
P 		202
B 467.3
(3)
P 		432.7
(1.5)
P 		331.9
(-3)
P 		462.9
(2.8)
P 		135
C 538.6
(3)
P 		419.2
(-3)
P 		450.3
(-1.4)
P 		454
(-1.3)
P 		119
avr 515.1
(3) 		406
(-1.6) 		373.2
(-3) 		428.8
(-0.6) 		142

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 376
1 2 3 4 5 6 range
A 396.3
(-2.5)
P 		555.7
(0.7)
P 		674.7
(3)
P 		562.4
(0.8)
P 		370.8
(-3)
P 		442.6
(-1.6)
P 		304
B 541.5
(-0.9)
P 		568.4
(-0.1)
P 		664.2
(3)
P 		477.3
(-3)
P 		480.5
(-2.9)
P 		488.7
(-2.6)
P 		187
C 490.5
(0.5)
P 		628.7
(3)
P 		459.8
(-0.1)
P 		553.9
(1.6)
P 		298.8
(-3)
P 		471.4
(0.1)
P 		330
avr 476.1
(-0.4) 		584.3
(2.6) 		599.6
(3) 		531.2
(1.1) 		383.4
(-3) 		467.6
(-0.7) 		216
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 196.4
(3)
P 		79.5
(-1.9)
P 		88.2
(-1.6)
P 		54.1
(-3)
P 		142
B 149
(3)
P 		106.9
(-0.5)
P 		76.9
(-3)
P 		98.6
(-1.2)
P 		72
C 178.3
(3)
P 		120.5
(-0.4)
P 		156.9
(1.7)
P 		76.6
(-3)
P 		102
avr 174.6
(3) 		102.3
(-1.4) 		107.3
(-1.1) 		76.4
(-3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 73.1
(-3)
P 		197.6
(3)
P 		146.9
(0.6)
P 		142.6
(0.3)
P 		111.9
(-1.1)
P 		107.8
(-1.3)
P 		125
B 188.4
(2.4)
P 		189.2
(2.5)
P 		161.3
(0)
P 		126.4
(-3)
P 		195.2
(3)
P 		182.2
(1.9)
P 		69
C 181.8
(2.4)
P 		191.8
(3)
P 		117
(-1.7)
P 		111.4
(-2.1)
P 		96.7
(-3)
P 		157.8
(0.9)
P 		95
avr 147.8
(-1.1) 		192.9
(3) 		141.7
(-1.6) 		126.8
(-3) 		134.6
(-2.3) 		149.3
(-1) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189347_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10482
Sequence Type Exemplar sequence
Representative Public ID T07A9.2 NCBI
Target Description T07A9.2 /REP_DB=WormBase Gene ID /WP=CE17211 /TR=O44413 /GB=AAK39275.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=tRNA synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067516(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067516 NCBI Caenorhabditis elegans glutamyl(E)/glutaminyl(Q) tRNA Synthetase family member (ers-3) (ers-3) mRNA, complete cds. 11/11 None
SNAP00000017757 ENSEMBL cdna:SNAP chromosome:CEL140:IV:388759:389855:-1 11/11 None
GENEFINDER00000017779 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:388759:390414:-1 11/11 None
T07A9.2 ENSEMBL cdna:known chromosome:CEL140:IV:388711:390414:-1 gene:T07A9.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:388758-390414(-) 100.0 100.0
Public Domain and Genome References
Gene Title tRNA synthetase
Gene Symbol ers-3
Ensembl T07A9.2
Entrez Gene 176860 Entrez gene
SwissProt O44413 EMBL-EBI
WormBase CE34997 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:247319_AT glutamate-tRNA ligase family protein / glutamyl-tRNA synthetase family protein at
CANINE_2:CFAAFFX.27043.1.S1_AT similar to CG4573-PA cfa
CANINE_2:CFAAFFX.27043.1.S1_S_AT similar to CG4573-PA cfa
CANINE_2:CFAAFFX.27045.1.S1_S_AT similar to CG4573-PA cfa
DROSOPHILA_2:1634404_AT dm
DROSGENOME1:142648_AT dm
CHICKEN:GGAAFFX.12671.1.S1_AT similar to KIAA1970 protein gga
CHICKEN:GGAAFFX.3818.1.S1_S_AT similar to KIAA1970 protein gga
MOE430A:1451471_AT RIKEN cDNA 3230401I01 gene mm
MOUSE430A_2:1451471_AT RIKEN cDNA 3230401I01 gene mm
MOUSE430_2:1451471_AT RIKEN cDNA 3230401I01 gene mm
MU11KSUBB:MSA.26400.0_S_AT RIKEN cDNA 3230401I01 gene mm
MG-U74BV2:107509_AT RIKEN cDNA 3230401I01 gene mm
MOE430A:1424774_S_AT RIKEN cDNA 3230401I01 gene mm
MOUSE430A_2:1424774_S_AT RIKEN cDNA 3230401I01 gene mm
MOUSE430_2:1424774_S_AT RIKEN cDNA 3230401I01 gene mm
MOE430A:1451472_AT RIKEN cDNA 3230401I01 gene mm
MOUSE430A_2:1451472_AT RIKEN cDNA 3230401I01 gene mm
MOUSE430_2:1451472_AT RIKEN cDNA 3230401I01 gene mm
MG-U74BV2:104968_AT RIKEN cDNA 3230401I01 gene (3230401I01Rik), mRNA mm
MU19KSUBA:TC17570_AT RIKEN cDNA 3230401I01 gene (3230401I01Rik), mRNA mm
MU19KSUBA:TC20427_AT RIKEN cDNA 3230401I01 gene (3230401I01Rik), mRNA mm
RAE230B:1395503_AT similar to RIKEN cDNA 3230401I01 (predicted) rn
RAT230_2:1395503_AT similar to RIKEN cDNA 3230401I01 (predicted) rn
RAE230B:1385318_AT similar to RIKEN cDNA 3230401I01 (predicted) rn
RAT230_2:1385318_AT similar to RIKEN cDNA 3230401I01 (predicted) rn
RAE230B:1398544_AT Similar to RIKEN cDNA 3230401I01 (predicted) rn
RAT230_2:1398544_AT Similar to RIKEN cDNA 3230401I01 (predicted) rn
YEAST_2:1775445_AT Mitochondrial glutamyl-tRNA synthetase, encoded by a nuclear gene Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6418 tRNA aminoacylation for protein translation inferred from electronic annotation QuickGO AmiGO
6424 glutamyl-tRNA aminoacylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4812 tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
4818 glutamate-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK39275 Glutamyl(e)/glutaminyl(q) trna synthetase protein 3 [Caenorhabditis elegans] ref|NP_499917.2| glutamyl(E)/glutaminyl(Q) tRNA Synthetase family member (ers-3) [Caenorhabditis elegans] 0.0
blast CAE67945 Hypothetical protein CBG13545 [Caenorhabditis briggsae] 0.0
blast AAK39275 Glutamyl(e)/glutaminyl(q) trna synthetase protein 3 [Caenorhabditis elegans] ref|NP_499917.2| glutamyl(E)/glutaminyl(Q) tRNA Synthetase family member (ers-3) [Caenorhabditis elegans] 1.0E-176
blast CAE67945 Hypothetical protein CBG13545 [Caenorhabditis briggsae] 1.0E-135
blast EAA08061 ENSANGP00000012789 [Anopheles gambiae str. PEST] ref|XP_312366.2| ENSANGP00000012789 [Anopheles gambiae str. PEST] 7.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR000924 EMBL-EBI Glutamyl-tRNA synthetase, class Ic 4.8E-115
Pfam IPR000924 EMBL-EBI Glutamyl-tRNA synthetase, class Ic 1.4E-45
Sequence
Target Sequence 
>C. ELEGANS:189347_AT
cttggtgctctaccggaagcggtacttaatctgatgatcagaaatggagccggtattcgg
aattttgatgccgaacacttttattcactggacgaaatgattgaacagtttgatttgagc
ctgctgggaagaaggaatttgctgctagattctgacgttttgcaaaaatactcgagaatg
gcttttcaaaaatcggatttcaaagaattgtatcccaggattattgatattttgaataaa
aaatcgaattactccacatcgcgagaagatattcaaaaaatcgtcactttcttaaaggca
aaggaagaaaactttggattcttgagctcattgagtacagaattcagttggttttttact
cgacctcaatcctcacagcttctcaaggaatctcatccaaatgtcgatctcagaaatatt
ttgaacagtcttctggaaattgaagttttcaactctgaatctcttgaatatctcgcgaag
aatcatcaacttaatctggcgaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTGGTGCTCTACCGGAAGCGGTAC 562 217 808 Antisense
GAAGCGGTACTTAATCTGATGATCA 619 343 823 Antisense
GATGCCGAACACTTTTATTCACTGG 339 415 874 Antisense
GAACAGTTTGATTTGAGCCTGCTGG 384 351 910 Antisense
TTGCTGCTAGATTCTGACGTTTTGC 592 703 946 Antisense
AATCGAATTACTCCACATCGCGAGA 504 167 1049 Antisense
AACTTTGGATTCTTGAGCTCATTGA 588 139 1117 Antisense
TGGTTTTTTACTCGACCTCAATCCT 170 551 1156 Antisense
GGAATCTCATCCAAATGTCGATCTC 66 533 1194 Antisense
CTCTGAATCTCTTGAATATCTCGCG 471 231 1260 Antisense
GAATCATCAACTTAATCTGGCGAAA 561 327 1287 Antisense
Sequence Source Affymetrix Proprietary Database