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[189344_s_at] cytochrome P450

Description F08F3.7 /REP_DB=WormBase Gene ID /WP=CE09262 /TR=Q27531 /GB=AAB04873.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 59      4grp_highest_8_8 > 27      4grp_high_8_8 > 57      6grp_highest_nonorm > 84      6grp_total > 49      4grp_total > 63      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 666
1 2 3 4 range
A 780.1
(1.2)
P 		551.8
(-1.4)
P 		938.1
(3)
P 		410.2
(-3)
P 		528
B 855.1
(1.8)
P 		539
(-3)
P 		936.4
(3)
P 		579.6
(-2.4)
P 		397
C 899.3
(1.3)
P 		522.6
(-2.5)
P 		1075.8
(3)
P 		468.8
(-3)
P 		607
avr 844.8
(1.3) 		537.8
(-2.4) 		983.4
(3) 		486.2
(-3) 		497

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 356
1 2 3 4 5 6 range
A 493.2
(-1)
P 		600.8
(1.5)
P 		510.3
(-0.6)
P 		409.5
(-3)
P 		560.5
(0.6)
P 		663
(3)
P 		254
B 645.3
(2.2)
P 		518.5
(-0.3)
P 		530.7
(-0.1)
P 		383.4
(-3)
P 		688.2
(3)
P 		643
(2.1)
P 		305
C 738.9
(3)
P 		474.2
(-2.8)
P 		479.5
(-2.7)
P 		464.3
(-3)
P 		562.8
(-0.8)
P 		668.3
(1.5)
P 		275
avr 625.8
(2.2) 		531.2
(-0.2) 		506.8
(-0.8) 		419.1
(-3) 		603.8
(1.6) 		658.1
(3) 		239
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 315
1 2 3 4 range
A 284.1
(3)
P 		119.8
(-1.4)
P 		245.2
(2)
P 		60
(-3)
P 		224
B 272.7
(3)
P 		133.1
(-2.6)
P 		217
(0.8)
P 		123.5
(-3)
P 		149
C 297.7
(1.4)
P 		150.2
(-1.6)
P 		374.9
(3)
P 		79
(-3)
P 		296
avr 284.8
(3) 		134.4
(-1.6) 		279
(2.8) 		87.5
(-3) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 189
1 2 3 4 5 6 range
A 90.9
(-3)
P 		213.7
(3)
P 		111.1
(-2)
P 		103.9
(-2.4)
P 		169.1
(0.8)
P 		161.4
(0.4)
P 		123
B 224.5
(1.1)
P 		172.6
(-0.6)
P 		128.9
(-2.1)
P 		101.5
(-3)
P 		279.6
(3)
P 		239.8
(1.7)
P 		178
C 273.9
(3)
P 		144.7
(-1.3)
P 		122
(-2)
P 		93.4
(-3)
P 		182.1
(-0.1)
P 		223.7
(1.3)
P 		181
avr 196.4
(2.2) 		177
(1.2) 		120.7
(-1.9) 		99.6
(-3) 		210.3
(3) 		208.3
(2.9) 		111
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189344_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11813
Sequence Type Exemplar sequence
Representative Public ID F08F3.7 NCBI
Target Description F08F3.7 /REP_DB=WormBase Gene ID /WP=CE09262 /TR=Q27531 /GB=AAB04873.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072034(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072034 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-14A5) (cyp-14A5) mRNA, complete cds. 11/11 None
SNAP00000026682 ENSEMBL cdna:SNAP chromosome:CEL140:V:5420894:5423076:-1 11/11 None
GENEFINDER00000026696 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5420894:5423076:-1 11/11 None
F08F3.7 ENSEMBL cdna:known chromosome:CEL140:V:5420851:5423117:-1 gene:F08F3.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5420893-5423076(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-14A5
Ensembl F08F3.7
Entrez Gene 178926 Entrez gene
SwissProt Q27531 EMBL-EBI
WormBase CE09262 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.8528.1.S1_AT similar to Cytochrome P450 2G1 (CYPIIG1) (P450-OLF1) (Olfactive) cfa
MOUSE430_2:1449565_AT cytochrome P450, family 2, subfamily g, polypeptide 1 mm
MOUSE430A_2:1449565_AT cytochrome P450, family 2, subfamily g, polypeptide 1 mm
MOE430A:1449565_AT cytochrome P450, family 2, subfamily g, polypeptide 1 mm
MU11KSUBB:MSA.41270.0_S_AT cytochrome P450, family 2, subfamily g, polypeptide 1 mm
RT-U34:M33296COMPLETE_SEQ_AT cytochrome P450, subfamily 2G, polypeptide 1 rn
RG-U34A:M33296COMPLETE_SEQ_AT cytochrome P450, subfamily 2G, polypeptide 1 rn
RT-U34:M31931CDS_S_AT cytochrome P450, subfamily 2G, polypeptide 1 rn
RG-U34A:M31931CDS_S_AT cytochrome P450, subfamily 2G, polypeptide 1 rn
RAE230A:1371142_AT cytochrome P450, subfamily 2G, polypeptide 1 rn
RAT230_2:1371142_AT cytochrome P450, subfamily 2G, polypeptide 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB04873 Cytochrome p450 protein 14A5 [Caenorhabditis elegans] ref|NP_504435.1| CYtochrome P450 family member (cyp-14A5) [Caenorhabditis elegans] 0.0
blast CAA90615 Hypothetical protein K09A11.3 [Caenorhabditis elegans] ref|NP_510204.1| CYtochrome P450 family member (cyp-14A2) [Caenorhabditis elegans] 0.0
blast CAA90617 Hypothetical protein K09A11.4 [Caenorhabditis elegans] ref|NP_510205.1| CYtochrome P450 family member (cyp-14A3) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
p450 pir:B37222 Animalia; CYP1A2; dog; pir:B37222; Canis familiaris; B37222 1.0E-149
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 7.7E-118
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504435.1 1 2-21
F08F3.7 1 2-21
SNAP00000026682 1 2-21
GENEFINDER00000026696 1 2-21
Sequence
Target Sequence 
>C. ELEGANS:189344_S_AT
aaatctatgcgcatcagtacttgatttctggctagctggaatggaaacagcctcgaattc
ccttcgttggcatttggcattcatgatgaaatatccagaagttcaagataaagttcgaaa
cgaaatttttgagaatataggcacggcgagactaccatccatgtctgacaaacaaaatat
gccttacactcaagcagtgatacatgaagttcagagatgctccaatatggttcccatttt
agcaactcatatgaacacagaagatgtccttgtcaaagaacacaacataccaactggaac
tctcttatttgctcaaatttggtcggttttgaaaaatgacccagttttcgaagagaactc
taaattcaacccggacagatatttgatgcctgacggaaaaacgttgaacaagactgtact
tgaacgaacaataccgttcagtgtcggaaagcgaaattgcgtgggcgaaggactggcccg
aatggagttgtttttgattttcagtgcgctcatccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATCTATGCGCATCAGTACTTGAT 81 117 870 Antisense
AGTACTTGATTTCTGGCTAGCTGGA 126 63 885 Antisense
CTCGAATTCCCTTCGTTGGCATTTG 376 229 921 Antisense
ATATAGGCACGGCGAGACTACCATC 555 19 1004 Antisense
GACTACCATCCATGTCTGACAAACA 213 373 1019 Antisense
GAGATGCTCCAATATGGTTCCCATT 340 395 1083 Antisense
ATGGTTCCCATTTTAGCAACTCATA 381 51 1096 Antisense
CTGGAACTCTCTTATTTGCTCAAAT 477 241 1163 Antisense
GAGAACTCTAAATTCAACCCGGACA 242 391 1222 Antisense
GAAGGACTGGCCCGAATGGAGTTGT 691 333 1336 Antisense
TTTGATTTTCAGTGCGCTCATCCAA 691 667 1362 Antisense
Sequence Source Affymetrix Proprietary Database