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[189343_at] cytochrome P450

Description T09H2.1 /REP_DB=WormBase Gene ID /WP=CE18231 /TR=O61935 /GB=AAC17779.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_total > 96      4grp_total > 02      6grp_cgc > 68      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2758
1 2 3 4 range
A 4.4
(-3)
A 		74.4
(-2.8)
A 		16.4
(-3)
A 		2127.7
(3)
P 		2123
B 40
(-2.9)
A 		38
(-2.9)
A 		13.9
(-3)
A 		2762.7
(3)
P 		2749
C 28.3
(-3)
A 		63.1
(-2.9)
A 		105.2
(-2.8)
A 		2327.4
(3)
P 		2299
avr 24.2
(-3) 		58.5
(-2.9) 		45.2
(-2.9) 		2405.9
(3) 		2382

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 952
1 2 3 4 5 6 range
A 4.7
(-3)
A 		3.5
(-3)
A 		25.1
(-2.9)
A 		384
(-0.6)
P 		363.1
(-0.7)
P 		955.1
(3)
P 		952
B 4.3
(-3)
A 		10.2
(-3)
A 		30
(-2.8)
A 		235.1
(-1.3)
P 		450.8
(0.2)
P 		832.1
(3)
P 		828
C 4.3
(-3)
A 		6.6
(-3)
A 		13.9
(-2.9)
A 		250.3
(-1.4)
P 		738.5
(1.7)
P 		936
(3)
P 		932
avr 4.4
(-3) 		6.8
(-3) 		23
(-2.9) 		289.8
(-1.1) 		517.5
(0.4) 		907.7
(3) 		903
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 587
1 2 3 4 range
A 1.6
(-3)
A 		16.1
(-2.7)
A 		4.3
(-2.9)
A 		311.4
(3)
P 		310
B 12.8
(-2.9)
A 		9.4
(-2.9)
A 		3.2
(-3)
A 		588.7
(3)
P 		586
C 9.4
(-3)
A 		18.1
(-2.9)
A 		36.7
(-2.6)
A 		392.4
(3)
P 		383
avr 7.9
(-3) 		14.5
(-2.9) 		14.7
(-2.9) 		430.8
(3) 		423

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 312
1 2 3 4 5 6 range
A 0.9
(-3)
A 		1.3
(-3)
A 		5.5
(-2.9)
A 		97.4
(-0.5)
P 		109.6
(-0.2)
P 		232.5
(3)
P 		232
B 1.5
(-3)
A 		3.4
(-3)
A 		7.3
(-2.9)
A 		62.3
(-1.8)
P 		183.2
(0.5)
P 		310.3
(3)
P 		309
C 1.6
(-3)
A 		2
(-3)
A 		3.5
(-3)
A 		50.3
(-2.1)
P 		239
(1.6)
P 		313.3
(3)
P 		312
avr 1.3
(-3) 		2.2
(-3) 		5.4
(-2.9) 		70
(-1.5) 		177.3
(0.7) 		285.4
(3) 		284
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189343_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15314
Sequence Type Exemplar sequence
Representative Public ID T09H2.1 NCBI
Target Description T09H2.1 /REP_DB=WormBase Gene ID /WP=CE18231 /TR=O61935 /GB=AAC17779.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071696(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071696 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-34A4) (cyp-34A4) mRNA, complete cds. 11/11 None
SNAP00000040818 ENSEMBL cdna:SNAP chromosome:CEL140:V:3949879:3951776:1 11/11 None
GENEFINDER00000040819 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3949879:3951772:1 11/11 None
T09H2.1 ENSEMBL cdna:known chromosome:CEL140:V:3949879:3951776:1 gene:T09H2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3949878-3951776(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-34A4
Ensembl T09H2.1
Entrez Gene 188350 Entrez gene
SwissProt O61935 EMBL-EBI
WormBase CE18231 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:260228_AT cytochrome P450, putative at
RT-U34:J04187_AT cytochrome P450, subfamily 2A, polypeptide 1 rn
RG-U34A:J04187_AT cytochrome P450, subfamily 2A, polypeptide 1 rn
RT-U34:J02669_S_AT cytochrome P450, subfamily 2A, polypeptide 1 rn
RG-U34A:J02669_S_AT cytochrome P450, subfamily 2A, polypeptide 1 rn
RAE230A:1369424_AT cytochrome P450, subfamily 2A, polypeptide 1 rn
RAT230_2:1369424_AT cytochrome P450, subfamily 2A, polypeptide 1 rn
RAE230A:1369275_S_AT cytochrome P450, subfamily 2A, polypeptide 1 rn
RAT230_2:1369275_S_AT cytochrome P450, subfamily 2A, polypeptide 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC17779 Hypothetical protein T09H2.1 [Caenorhabditis elegans] ref|NP_504097.1| CYtochrome P450 family member (cyp-34A4) [Caenorhabditis elegans] 0.0
blast CAE58176 Hypothetical protein CBG01268 [Caenorhabditis briggsae] 0.0
blast CAB03339 Hypothetical protein T10H4.11 [Caenorhabditis elegans] ref|NP_506788.1| CYtochrome P450 family member (cyp-34A2) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
p450 CPA2_RAT Animalia; CYP2A2; rat; CPA2_RAT; Rattus sp.; P15149 1.0E-153
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.6E-32
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.9E-60
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504097.1 1 2-20
T09H2.1 1 2-20
SNAP00000040818 1 2-20
GENEFINDER00000040819 1 2-20
Sequence
Target Sequence 
>C. ELEGANS:189343_AT
atgtacgacctgtggattgctggccaagaaaccacttctacaactctatcatgggcctgc
gcctgtcttctgaaccatccggaagttgtgaaaattgcacgagaagagctggtccatttg
actggagggcatcggagtatttcattgactgacaaaacttcaacgccttatctcaatgct
gttattaatgaagttcaacgaattgcttcaattctcaacgtgaacattttccgtcaaaca
tccgaggatacaactgtcaacggacaacctattgctgcaggaaccgctctgaccacacat
ttatctttaattcatactgacgagaatctgtttcaaaatcacaccgaattccggccggag
cgctttttggagaataataacctggagaagaagctgatcccatttggaatcggaaaacgt
gcatgcctcggagaatcattggctcgagctgagctctacttagtaaccggaaatatgatc
ttggactatgatcttcagccgatcggagaagttcctcaaatcaagacgacgtcaccatgc
ggaattatgaagcggccaccagtcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTACGACCTGTGGATTGCTGGCC 6 47 916 Antisense
ACTTCAACGCCTTATCTCAATGCTG 110 103 1072 Antisense
AACATTTTCCGTCAAACATCCGAGG 596 137 1138 Antisense
AACCTATTGCTGCAGGAACCGCTCT 224 147 1181 Antisense
GCTCTGACCACACATTTATCTTTAA 464 299 1201 Antisense
CAAAATCACACCGAATTCCGGCCGG 451 189 1249 Antisense
CGGCCGGAGCGCTTTTTGGAGAATA 372 241 1267 Antisense
ATCGGAAAACGTGCATGCCTCGGAG 384 33 1324 Antisense
CGAGCTGAGCTCTACTTAGTAACCG 387 251 1360 Antisense
CAAGACGACGTCACCATGCGGAATT 699 181 1437 Antisense
GAATTATGAAGCGGCCACCAGTCTA 588 323 1457 Antisense
Sequence Source Affymetrix Proprietary Database