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[189340_at] RNA helicase

Description EEED8.5 /REP_DB=WormBase Gene ID /WP=CE01889 /TR=SW:Q09530 /GB=AAC46765.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=RNA helicase
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 46      4grp_highest_8_8 > 44      4grp_high_8_8 > 08      6grp_highest_nonorm > 75      6grp_total > 45      4grp_total > 31      6grp_highest_sub > Cu      6grp_cgc > 35      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 636
1 2 3 4 range
A 955.9
(3)
P 		599
(-3)
P 		637.4
(-2.4)
P 		792.2
(0.2)
P 		357
B 811.4
(-0.7)
P 		714.2
(-2.6)
P 		693.3
(-3)
P 		1006.7
(3)
P 		313
C 1235
(3)
P 		604.6
(-3)
P 		806
(-1.1)
P 		737.8
(-1.7)
P 		630
avr 1000.8
(3) 		639.3
(-3) 		712.2
(-1.8) 		845.6
(0.4) 		362

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 505
1 2 3 4 5 6 range
A 854.6
(3)
P 		836.8
(2.6)
P 		773
(1.2)
P 		580.2
(-3)
P 		682.9
(-0.8)
P 		752.3
(0.8)
P 		274
B 954.6
(2.2)
P 		909.9
(1.6)
P 		1009.5
(3)
P 		766.3
(-0.4)
P 		578.2
(-3)
P 		918.9
(1.7)
P 		431
C 797.7
(0.5)
P 		937.7
(2.2)
P 		822.6
(0.8)
P 		639.7
(-1.4)
P 		504.5
(-3)
P 		1003.5
(3)
P 		499
avr 869
(2.5) 		894.8
(3) 		868.4
(2.5) 		662.1
(-1.6) 		588.5
(-3) 		891.6
(2.9) 		306
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 293
1 2 3 4 range
A 348
(3)
P 		130
(-2.6)
P 		166.6
(-1.7)
P 		116
(-3)
P 		232
B 258.8
(3)
P 		176.4
(-2)
P 		160.6
(-3)
P 		214.5
(0.3)
P 		98
C 408.8
(3)
P 		173.8
(-2)
P 		280.9
(0.3)
P 		124.4
(-3)
P 		284
avr 338.5
(3) 		160.1
(-2.7) 		202.7
(-1.4) 		151.6
(-3) 		187

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 214
1 2 3 4 5 6 range
A 157.5
(-2.6)
P 		297.6
(3)
P 		168.3
(-2.2)
P 		147.2
(-3)
P 		206.1
(-0.7)
P 		183.2
(-1.6)
P 		150
B 332.2
(2.6)
P 		302.9
(1.3)
P 		245.1
(-1.2)
P 		203
(-3)
P 		234.9
(-1.6)
P 		342.6
(3)
P 		140
C 295.7
(1.8)
P 		286.1
(1.6)
P 		209.4
(-0.7)
P 		128.7
(-3)
P 		163.2
(-2)
P 		335.9
(3)
P 		207
avr 261.8
(1.5) 		295.5
(3) 		207.6
(-0.9) 		159.6
(-3) 		201.4
(-1.2) 		287.2
(2.6) 		136
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189340_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5104
Sequence Type Exemplar sequence
Representative Public ID EEED8.5 NCBI
Target Description EEED8.5 /REP_DB=WormBase Gene ID /WP=CE01889 /TR=SW:Q09530 /GB=AAC46765.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=RNA helicase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062618(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062618 NCBI Caenorhabditis elegans Masculinisation Of Germline family member (mog-5) (mog-5) mRNA, complete cds. 11/11 None
SNAP00000023169 ENSEMBL cdna:SNAP chromosome:CEL140:II:5394308:5405596:1 11/11 None
GENEFINDER00000023193 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5399509:5405596:1 11/11 None
EEED8.5 ENSEMBL cdna:known chromosome:CEL140:II:5399508:5405719:1 gene:EEED8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5399506-5405594(+) 98.61 100.0
Public Domain and Genome References
Gene Title RNA helicase
Gene Symbol mog-5
Ensembl EEED8.5
Entrez Gene 173920 Entrez gene
SwissProt Q09530 EMBL-EBI
WormBase CE01889 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:12603_G_AT ATP-dependent RNA helicase, putative at
ATGENOME1:12602_AT ATP-dependent RNA helicase, putative at
ATH1-121501:257310_AT ATP-dependent RNA helicase, putative at
CANINE_2:CFAAFFX.22452.1.S1_S_AT similar to DEAH (Asp-Glu-Ala-His) box polypeptide 8 cfa
DROSOPHILA_2:1640151_AT dm
DROSGENOME1:141695_AT dm
CHICKEN:GGAAFFX.5323.1.S1_S_AT similar to ATP-dependent helicase DHX8 (RNA helicase HRH1) (DEAH-box protein 8) gga
CHICKEN:GGAAFFX.5323.2.S1_AT similar to ATP-dependent helicase DHX8 (RNA helicase HRH1) (DEAH-box protein 8) gga
CHICKEN:GGAAFFX.5323.3.S1_AT similar to ATP-dependent helicase DHX8 (RNA helicase HRH1) (DEAH-box protein 8) gga
CHICKEN:GGAAFFX.5323.3.S1_S_AT similar to ATP-dependent helicase DHX8 (RNA helicase HRH1) (DEAH-box protein 8) gga
HG-U133_PLUS_2:227079_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HG-U133B:227079_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HG-U95AV2:744_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HC-G110:744_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HG-U133_PLUS_2:203334_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HG-U133A_2:203334_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HG-U133A:203334_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HG-FOCUS:203334_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HU35KSUBD:RC_AA434442_S_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HUGENEFL:D50487_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
U133_X3P:G4826689_3P_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
U133_X3P:HS.171872.2.S1_3P_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
U133_X3P:HS.171872.1.S1_3P_S_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HG-U133_PLUS_2:231184_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HG-U133B:231184_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HG-U95B:43074_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
HG-U95E:87989_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 hs
MG-U74BV2:111987_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 mm
MG-U74BV2:111988_G_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 mm
MG-U74BV2:111989_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 mm
MOE430A:1426629_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 mm
MOUSE430A_2:1426629_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 mm
MOUSE430_2:1426629_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 mm
MU19KSUBB:TC34583_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 (Dhx8), mRNA mm
MU19KSUBB:TC34583_G_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 (Dhx8), mRNA mm
MU19KSUBC:TC36104_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 (Dhx8), mRNA mm
MU19KSUBC:TC36104_G_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 (Dhx8), mRNA mm
RAE230B:1396052_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 (predicted) rn
RAT230_2:1396052_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 (predicted) rn
RAE230B:1391742_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 (predicted) rn
RAT230_2:1391742_AT DEAH (Asp-Glu-Ala-His) box polypeptide 8 (predicted) rn
YEAST_2:1770419_AT DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC46765 Masculinisation of germline protein 5 [Caenorhabditis elegans] ref|NP_495019.1| Masculinisation Of Germline family member (mog-5) [Caenorhabditis elegans] gb|AAG01332.1| sex determining protein MOG-5 [Caenorhabditis elegans] sp|Q09530|MOG5_CAEEL Probable pre-mRNA splicing factor ATP-dependent RNA helicase mog-5 (Sex determination protein mog-5) (Masculinization of germ line protein 5) 0.0
blast CAE59095 Hypothetical protein CBG02387 [Caenorhabditis briggsae] 0.0
blast EAA04624 ENSANGP00000015955 [Anopheles gambiae str. PEST] ref|XP_308573.2| ENSANGP00000015955 [Anopheles gambiae str. PEST] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007502 EMBL-EBI Helicase-associated region 3.5E-48
Pfam IPR003029 EMBL-EBI RNA binding S1 2.6E-11
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.0E-15
Pfam IPR007502 EMBL-EBI Helicase-associated region 3.5E-48
Pfam IPR003029 EMBL-EBI RNA binding S1 2.6E-11
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.0E-15
Sequence
Target Sequence 
>C. ELEGANS:189340_AT
gaaggcgaaattccatcaaccagaaggagatcatttgacacttctagcggtttacaattc
atggaaaaaccatcatttctcacaaccatggtgtttcgaaaatttcattcaagtgagaag
tatgaagagagctcaggacattcgtaagcaattactcggaatcatggatcgtcataaact
tctgatggtcagttgtggacgggatgtatcgagagttcagaaggctatttgctctggatt
cttccgaaatgctgcaaaacgtgatccacaggaaggatatcgaacattgactgatggaca
gaatgtttatattcatccatcatcagcatgtttccaacaacaaccagaatgggttgtata
tcatgaactcgtcatgacaactaaggaatatatgagagaagtcaccgcaatcgatccaaa
atggcttgttgaatttgcaccgtcattcttcaaaatcggagattctaccaaattatcaac
attcaaacgaaaccagaaaatcgatccgttgttcgacaagtatgcagatgcaaatgcctg
gcgtatcacacgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGGCGAAATTCCATCAACCAGAA 484 333 3030 Antisense
TGACACTTCTAGCGGTTTACAATTC 78 571 3065 Antisense
AAAACCATCATTTCTCACAACCATG 672 131 3095 Antisense
GAGAGCTCAGGACATTCGTAAGCAA 20 395 3156 Antisense
GGATCGTCATAAACTTCTGATGGTC 118 513 3195 Antisense
GAAATGCTGCAAAACGTGATCCACA 72 363 3275 Antisense
GTTTATATTCATCCATCATCAGCAT 500 449 3334 Antisense
GAGAAGTCACCGCAATCGATCCAAA 587 391 3425 Antisense
AATGGCTTGTTGAATTTGCACCGTC 154 163 3449 Antisense
ATTTGCACCGTCATTCTTCAAAATC 549 17 3462 Antisense
GCAAATGCCTGGCGTATCACACGTG 339 317 3559 Antisense
Sequence Source Affymetrix Proprietary Database