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[189338_at]

Description ZK892.1 /REP_DB=WormBase Gene ID /WP=CE24743 /CHR=2 /FEA=Sanger Annotation /DEF=locus:lec-3 galactoside-binding lectin (HINXTON) SW:Q09581
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 16      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 01      6grp_total > 06      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2330
1 2 3 4 range
A 2904.4
(3)
P 		2739.7
(2.6)
P 		1836.6
(0.1)
P 		687.6
(-3)
P 		2217
B 2686.9
(3)
P 		2634.1
(2.8)
P 		2137.2
(1.4)
P 		687.3
(-3)
P 		2000
C 2965.4
(3)
P 		2673.2
(2.2)
P 		1878.6
(0.2)
P 		635.8
(-3)
P 		2330
avr 2852.2
(3) 		2682.3
(2.5) 		1950.8
(0.5) 		670.2
(-3) 		2182

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3084
1 2 3 4 5 6 range
A 2573.6
(1.1)
P 		3242.6
(3)
P 		1309.5
(-2.4)
P 		1174
(-2.8)
P 		1090.2
(-3)
P 		1323.7
(-2.3)
P 		2152
B 2418.5
(3)
P 		1885.4
(1)
P 		1178.4
(-1.7)
P 		991.5
(-2.4)
P 		1207.8
(-1.6)
P 		830.4
(-3)
P 		1588
C 3816
(3)
P 		1694.6
(-1.1)
P 		1711.6
(-1.1)
P 		732.1
(-3)
P 		1775.9
(-1)
P 		917.5
(-2.6)
P 		3084
avr 2936
(3) 		2274.2
(1) 		1399.8
(-1.7) 		965.9
(-3) 		1358
(-1.8) 		1023.9
(-2.8) 		1970
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 957
1 2 3 4 range
A 1057.5
(3)
P 		594.7
(0.1)
P 		480.1
(-0.6)
P 		100.6
(-3)
P 		957
B 856.8
(3)
P 		650.6
(1.3)
P 		495.2
(-0.1)
P 		146.5
(-3)
P 		710
C 981.6
(3)
P 		768.6
(1.5)
P 		654.8
(0.8)
P 		107.2
(-3)
P 		874
avr 965.3
(3) 		671.3
(0.9) 		543.4
(0) 		118.1
(-3) 		847

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1267
1 2 3 4 5 6 range
A 474.4
(-1.7)
P 		1153.2
(3)
P 		285.2
(-3)
P 		297.8
(-2.9)
P 		329
(-2.7)
P 		322.3
(-2.7)
P 		868
B 841.5
(3)
P 		627.6
(0.8)
P 		286.2
(-2.8)
P 		262.6
(-3)
P 		490.7
(-0.6)
P 		309.6
(-2.5)
P 		579
C 1414.4
(3)
P 		517.1
(-1.2)
P 		435.6
(-1.6)
P 		147.2
(-3)
P 		574.6
(-1)
P 		307.1
(-2.2)
P 		1267
avr 910.1
(3) 		766
(1.7) 		335.7
(-2.1) 		235.9
(-3) 		464.8
(-1) 		313
(-2.3) 		674
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189338_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2706
Sequence Type Exemplar sequence
Representative Public ID ZK892.1 NCBI
Target Description ZK892.1 /REP_DB=WormBase Gene ID /WP=CE24743 /CHR=2 /FEA=Sanger Annotation /DEF=locus:lec-3 galactoside-binding lectin (HINXTON) SW:Q09581
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063758(11), NM_001027360(9), NM_001027362(11), NM_001027361(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063758 NCBI Caenorhabditis elegans gaLECtin family member (lec-3) (lec-3) mRNA, complete cds. 11/11 A 
NM_001027360 NCBI Caenorhabditis elegans gaLECtin family member (lec-3) (lec-3) mRNA, complete cds. 9/11 None
NM_001027362 NCBI Caenorhabditis elegans gaLECtin family member (lec-3) (lec-3) mRNA, complete cds. 11/11 None
NM_001027361 NCBI Caenorhabditis elegans gaLECtin family member (lec-3) (lec-3) mRNA, complete cds. 10/11 None
GENEFINDER00000002403 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9991071:9999895:1 10/11 None
ZK892.1a ENSEMBL cdna:known chromosome:CEL140:II:9991280:10000248:1 gene:ZK892.1 11/11 A 
ZK892.1b ENSEMBL cdna:known chromosome:CEL140:II:9991320:10000244:1 gene:ZK892.1 9/11 A 
ZK892.1d ENSEMBL cdna:known chromosome:CEL140:II:9993531:10000256:1 gene:ZK892.1 11/11 A 
ZK892.1c ENSEMBL cdna:known chromosome:CEL140:II:9993845:10000248:1 gene:ZK892.1 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000002392 2/11 Cross Hyb Matching Probes None
SNAP00000002393 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174557 5 4 NM_001027360 174166_at (11), 189338_at (9)
NM_001027361 174166_at (11), 189338_at (10)
NM_001027362 174166_at (11), 189338_at (11)
NM_063758 174166_at (11), 189338_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9994183-9999892(+) 97.65 97.65
Public Domain and Genome References
Ensembl ZK892.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD57721 Hypothetical protein ZK892.1d [Caenorhabditis elegans] ref|NP_001022533.1| gaLECtin family member (lec-3) [Caenorhabditis elegans] 0.0
blast CAA88570 Hypothetical protein ZK892.1a [Caenorhabditis elegans] ref|NP_496159.1| gaLECtin family member (lec-3) [Caenorhabditis elegans] sp|Q09581|LEC3_CAEEL 32 kDa beta-galactoside-binding lectin lec-3 (32 kDa GBP) dbj|BAB11969.1| galectin LEC-3 [Caenorhabditis elegans] 1.0E-174
blast CAD57721 Hypothetical protein ZK892.1d [Caenorhabditis elegans] ref|NP_001022533.1| gaLECtin family member (lec-3) [Caenorhabditis elegans] 1.0E-171
blast CAD57721 Hypothetical protein ZK892.1d [Caenorhabditis elegans] ref|NP_001022533.1| gaLECtin family member (lec-3) [Caenorhabditis elegans] 1.0E-167
blast CAA88570 Hypothetical protein ZK892.1a [Caenorhabditis elegans] ref|NP_496159.1| gaLECtin family member (lec-3) [Caenorhabditis elegans] sp|Q09581|LEC3_CAEEL 32 kDa beta-galactoside-binding lectin lec-3 (32 kDa GBP) dbj|BAB11969.1| galectin LEC-3 [Caenorhabditis elegans] 1.0E-167
blast CAC42392 Hypothetical protein ZK892.1b [Caenorhabditis elegans] ref|NP_001022531.1| gaLECtin family member (lec-3) [Caenorhabditis elegans] 1.0E-166
Protein Domains
Database ID Description E-Value
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 5.6E-32
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 1.3E-15
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 6.3E-29
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 5.6E-32
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 4.2E-46
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 1.1E-50
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 5.6E-32
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 1.3E-15
Pfam IPR001079 EMBL-EBI Galectin, galactose-binding lectin 6.3E-29
Sequence
Target Sequence 
>C. ELEGANS:189338_AT
tttcgatatccgcattcgggctcacgattccaagttccaagtgtccattaaccacaaaga
ggtgaagaacttcgagcaccgtatcccactcaactcggtgtcccatttgtccatcgatgg
ggatgttgtcctgaaccacgttcaatggggaggaaagtactacccagtcccatatgaatc
cggaatcgccgccgatggattggtcccaggaaagactctcgttgtctatggaactccaga
gaagaaggccaagaaattcaatattaaccttctcaagaagaacggagatattgctcttca
tttcaatccacgtttcgatgagaaggcaaatggatttatgtgcgctaagccgacccctgg
aagtgtcgtccgtaactcgcttgtcaatggagaatggggaaatgaggaacgagagggaaa
gaacccattcgagagattgacggctttcgatttggagatcagaaacgaagagtttgcttt
ccagatctttgtcaacggagaacgcttcgcctcatacgcccaccgcgtcgacccacacga
catcgccggacttcagattcaggga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTCGATATCCGCATTCGGGCTCAC 16 663 288 Antisense
ACGATTCCAAGTTCCAAGTGTCCAT 162 93 311 Antisense
TGAAGAACTTCGAGCACCGTATCCC 119 573 350 Antisense
CACCGTATCCCACTCAACTCGGTGT 402 199 364 Antisense
ATATGAATCCGGAATCGCCGCCGAT 317 21 459 Antisense
GACTCTCGTTGTCTATGGAACTCCA 637 371 501 Antisense
GGATTTATGTGCGCTAAGCCGACCC 27 511 619 Antisense
TAAGCCGACCCCTGGAAGTGTCGTC 363 627 633 Antisense
CGTCCGTAACTCGCTTGTCAATGGA 141 245 654 Antisense
GTTTGCTTTCCAGATCTTTGTCAAC 341 451 759 Antisense
CATCGCCGGACTTCAGATTCAGGGA 101 211 828 Antisense
Sequence Source Affymetrix Proprietary Database