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[189336_at] Cytochrome P450

Description K09A11.3 /REP_DB=WormBase Gene ID /WP=CE03474 /TR=Q27505 /GB=CAA90615.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=Cytochrome P450
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 62      4grp_high_8_8 > 38      6grp_highest_nonorm > 90      6grp_total > 61      4grp_total > 41      6grp_highest_sub > 03      6grp_cgc > 93      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 45.3
(-3)
A 		75.4
(1.5)
P 		74.1
(1.3)
P 		85.8
(3)
P 		41
B 42.4
(-3)
A 		96.6
(3)
M 		79.9
(1.2)
P 		74.4
(0.5)
P 		54
C 40.2
(-3)
P 		89.3
(3)
A 		84.8
(2.5)
P 		56.3
(-1)
A 		49
avr 42.6
(-3) 		87.1
(3) 		79.6
(2) 		72.2
(1) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 146
1 2 3 4 5 6 range
A 128
(-0.6)
P 		111.6
(-1.4)
P 		205.2
(3)
P 		197.3
(2.6)
P 		83.5
(-2.7)
P 		77.4
(-3)
P 		128
B 76
(-2.2)
P 		72.1
(-2.4)
P 		189.5
(3)
P 		152.6
(1.3)
P 		119.3
(-0.2)
P 		59
(-3)
P 		131
C 88.8
(-1.9)
P 		114
(-0.8)
P 		201.6
(3)
P 		174.5
(1.8)
P 		105.9
(-1.2)
P 		64
(-3)
P 		138
avr 97.6
(-1.6) 		99.2
(-1.5) 		198.8
(3) 		174.8
(1.9) 		102.9
(-1.4) 		66.8
(-3) 		132
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 16.5
(0.4)
A 		16.4
(0.4)
P 		19.4
(3)
P 		12.6
(-3)
P 		7
B 13.5
(-3)
A 		23.9
(3)
M 		18.5
(-0.1)
P 		15.8
(-1.7)
P 		10
C 13.3
(-1.9)
P 		25.7
(1.9)
A 		29.5
(3)
P 		9.5
(-3)
A 		20
avr 14.4
(-1.9) 		22
(2.7) 		22.5
(3) 		12.6
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 23.6
(-2.1)
P 		39.7
(1)
P 		44.7
(2)
P 		50.1
(3)
P 		25.2
(-1.8)
P 		18.8
(-3)
P 		31
B 26.4
(-2)
P 		24
(-2.5)
P 		46
(2.4)
P 		40.4
(1.2)
P 		48.5
(3)
P 		22
(-3)
P 		27
C 32.9
(-0.7)
P 		34.8
(-0.3)
P 		51.3
(3)
P 		35.1
(-0.3)
P 		34.3
(-0.4)
P 		21.4
(-3)
P 		30
avr 27.6
(-1.4) 		32.8
(-0.3) 		47.3
(3) 		41.9
(1.8) 		36
(0.5) 		20.7
(-3) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189336_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17599
Sequence Type Exemplar sequence
Representative Public ID K09A11.3 NCBI
Target Description K09A11.3 /REP_DB=WormBase Gene ID /WP=CE03474 /TR=Q27505 /GB=CAA90615.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=Cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077803(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077803 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-14A2) (cyp-14A2) mRNA, complete cds. 11/11 None
SNAP00000010026 ENSEMBL cdna:SNAP chromosome:CEL140:X:13267525:13269593:-1 11/11 None
GENEFINDER00000010029 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:13267525:13268252:-1 11/11 None
K09A11.3 ENSEMBL cdna:known chromosome:CEL140:X:13267525:13269593:-1 gene:K09A11.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13267524-13269593(-) 100.0 100.0
Public Domain and Genome References
Gene Title Cytochrome P450
Gene Symbol cyp-14A2
Ensembl K09A11.3
Entrez Gene 187184 Entrez gene
SwissProt Q27505 EMBL-EBI
WormBase CE03474 Wormbase
Functional Annotations
Ortholog
ID Title Organism
MG-U74AV2:94539_F_AT RIKEN cDNA 9030605E09 gene mm
MOE430A:1435853_AT RIKEN cDNA 9030605E09 gene mm
MOUSE430A_2:1435853_AT RIKEN cDNA 9030605E09 gene mm
MOUSE430_2:1435853_AT RIKEN cDNA 9030605E09 gene mm
RT-U34:J02869MRNA_S_AT cytochrome P450, family 2, subfamily d, polypeptide 9 rn
RG-U34A:J02869MRNA_S_AT cytochrome P450, family 2, subfamily d, polypeptide 9 rn
RAE230A:1370377_AT cytochrome P450, family 2, subfamily d, polypeptide 9 rn
RAT230_2:1370377_AT cytochrome P450, family 2, subfamily d, polypeptide 9 rn
RT-U34:J02869MRNA_S_AT cytochrome P450, family 2, subfamily d, polypeptide 10 rn
RG-U34A:J02869MRNA_S_AT cytochrome P450, family 2, subfamily d, polypeptide 10 rn
RAE230A:1370377_AT cytochrome P450, family 2, subfamily d, polypeptide 10 rn
RAT230_2:1370377_AT cytochrome P450, family 2, subfamily d, polypeptide 10 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90615 Hypothetical protein K09A11.3 [Caenorhabditis elegans] ref|NP_510204.1| CYtochrome P450 family member (cyp-14A2) [Caenorhabditis elegans] 0.0
blast CAA90617 Hypothetical protein K09A11.4 [Caenorhabditis elegans] ref|NP_510205.1| CYtochrome P450 family member (cyp-14A3) [Caenorhabditis elegans] 0.0
blast AAB04873 Cytochrome p450 protein 14A5 [Caenorhabditis elegans] ref|NP_504435.1| CYtochrome P450 family member (cyp-14A5) [Caenorhabditis elegans] 0.0
blast CAA90615 Hypothetical protein K09A11.3 [Caenorhabditis elegans] ref|NP_510204.1| CYtochrome P450 family member (cyp-14A2) [Caenorhabditis elegans] 1.0E-118
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-154
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-151
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.6E-117
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.1E-111
Pfam IPR001128 EMBL-EBI Cytochrome P450 5.8E-72
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510204.1 1 2-21
K09A11.3 1 2-21
SNAP00000010026 1 2-21
Sequence
Target Sequence 
>C. ELEGANS:189336_AT
gaacaacctatgtgcatcagttatcgatttctggctggctggaatggaaaccacttctaa
ctctctccgctggcatttggcattcatgatgaaatatccagaaattcaagataaagtccg
gaaggaaatacttgataatgtaggaactgctagattaccatcaatgtcagacaaaccaaa
tatgccgtacactcaagcagtcatccatgaagttcaaagatgctcaaacatgataccaat
tcttgggactcatacaaaccgagatgacattctacttaaagggaagaaaataccaactgg
tacccttgtatttgcacaaatttggtcagttctgaaaaatgacccagttttcgaagagag
ctctaaattcaaccctgatagatatttaatggccgatggaaaaacaataaataagtctgt
attagaacgaacaattcccttcagtgtcggaaagcgaaattgcgttggagaaggattggc
ccgcatggagctcttcttgatttttagtgcattgatccaaaaatacgagttcattccaaa
aactaatattgatgtcaaaccggtctgcggagcagtcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACAACCTATGTGCATCAGTTATC 276 353 867 Antisense
GTTATCGATTTCTGGCTGGCTGGAA 620 447 886 Antisense
ATGGAAACCACTTCTAACTCTCTCC 228 53 910 Antisense
GAACTGCTAGATTACCATCAATGTC 400 349 1010 Antisense
TGCCGTACACTCAAGCAGTCATCCA 3 587 1049 Antisense
TACCAACTGGTACCCTTGTATTTGC 131 651 1157 Antisense
AAGAGAGCTCTAAATTCAACCCTGA 504 151 1220 Antisense
GAACGAACAATTCCCTTCAGTGTCG 313 345 1291 Antisense
GTTGGAGAAGGATTGGCCCGCATGG 708 435 1330 Antisense
GGAGCTCTTCTTGATTTTTAGTGCA 549 521 1353 Antisense
GTCAAACCGGTCTGCGGAGCAGTCC 297 461 1420 Antisense
Sequence Source Affymetrix Proprietary Database