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[189334_at] cytochrome P450

Description F41B5.4 /REP_DB=WormBase Gene ID /WP=CE10222 /TR=O16670 /GB=AAG24106.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_total > 68      4grp_total > 20      6grp_cgc > 39      6grp_cgc_sub > Cu      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 140
1 2 3 4 range
A 105.5
(-1.4)
A 		112.5
(-1.1)
A 		67.9
(-3)
A 		208.3
(3)
P 		140
B 115.8
(-0.7)
A 		102.2
(-1.5)
A 		73.2
(-3)
A 		186.6
(3)
P 		113
C 116.7
(-0.6)
A 		127.4
(0.1)
A 		78.9
(-3)
A 		173.2
(3)
P 		94
avr 112.7
(-1) 		114
(-0.9) 		73.3
(-3) 		189.4
(3) 		116

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 103
(-2)
A 		113.4
(-1.6)
A 		79
(-3)
A 		153.8
(0)
A 		129.3
(-1)
P 		226.3
(3)
P 		147
B 124.7
(-0.2)
M 		101.6
(-1.4)
A 		69.7
(-3)
A 		81.7
(-2.4)
A 		110.1
(-0.9)
P 		185.9
(3)
P 		116
C 142.8
(1.3)
A 		91.6
(-1.9)
A 		108.2
(-0.9)
A 		73.6
(-3)
A 		108.8
(-0.8)
A 		170.2
(3)
P 		97
avr 123.5
(-0.9) 		102.2
(-2.1) 		85.6
(-3) 		103
(-2) 		116.1
(-1.3) 		194.1
(3) 		109
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 38.4
(3)
A 		24.4
(-1.1)
A 		17.8
(-3)
A 		30.5
(0.7)
P 		21
B 36.9
(2.2)
A 		25.2
(-0.8)
A 		17
(-3)
A 		39.8
(3)
P 		23
C 38.6
(3)
A 		36.6
(1.9)
A 		27.5
(-3)
A 		29.2
(-2.1)
P 		11
avr 38
(3) 		28.7
(-0.2) 		20.8
(-3) 		33.2
(1.3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 19
(-2.7)
A 		40.3
(0.7)
A 		17.2
(-3)
A 		39
(0.5)
A 		39
(0.5)
P 		55.1
(3)
P 		38
B 43.4
(0)
M 		33.8
(-1.1)
A 		16.9
(-3)
A 		21.7
(-2.5)
A 		44.7
(0.2)
P 		69.3
(3)
P 		52
C 52.9
(2.4)
A 		28
(-1.1)
A 		27.5
(-1.2)
A 		14.8
(-3)
A 		35.2
(-0.1)
A 		57
(3)
P 		42
avr 38.4
(-0.3) 		34
(-1) 		20.5
(-3) 		25.2
(-2.3) 		39.6
(-0.1) 		60.5
(3) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189334_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15458
Sequence Type Exemplar sequence
Representative Public ID F41B5.4 NCBI
Target Description F41B5.4 /REP_DB=WormBase Gene ID /WP=CE10222 /TR=O16670 /GB=AAG24106.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071214(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071214 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-33C3) (cyp-33C3) mRNA, complete cds. 11/11 None
SNAP00000036000 ENSEMBL cdna:SNAP chromosome:CEL140:V:2242804:2244610:1 11/11 None
GENEFINDER00000036015 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2243126:2244610:1 11/11 None
F41B5.4 ENSEMBL cdna:known chromosome:CEL140:V:2242606:2244693:1 gene:F41B5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2242803-2244610(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-33C3
Ensembl F41B5.4
Entrez Gene 3565571 Entrez gene
SwissProt O16670 EMBL-EBI
WormBase CE10222 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGA.14761.1.A1_AT similar to cytochrome P450 2K5 gga
CHICKEN:GGAAFFX.26059.1.S1_AT similar to cytochrome P450 2K5 gga
CHICKEN:GGAAFFX.26059.3.S1_S_AT similar to cytochrome P450 2K5 gga
MOE430A:1449479_AT cytochrome P450, family 2, subfamily b, polypeptide 13 mm
MOUSE430A_2:1449479_AT cytochrome P450, family 2, subfamily b, polypeptide 13 mm
MOUSE430_2:1449479_AT cytochrome P450, family 2, subfamily b, polypeptide 13 mm
MU11KSUBA:M60358_AT cytochrome P450, family 2, subfamily b, polypeptide 13 mm
MU11KSUBB:MSA.134.0_S_AT cytochrome P450, family 2, subfamily b, polypeptide 13 mm
MG-U74AV2:102820_AT cytochrome P450, family 2, subfamily b, polypeptide 13 mm
RG-U34C:RC_AA818412_F_AT cytochrome P450 CYP2B21 rn
RAE230A:1387993_AT cytochrome P450 CYP2B21 rn
RAT230_2:1387993_AT cytochrome P450 CYP2B21 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAG24106 Cytochrome p450 family protein 33C3 [Caenorhabditis elegans] ref|NP_503615.1| CYtochrome P450 family member (cyp-33C3) [Caenorhabditis elegans] 0.0
blast AAB65888 Cytochrome p450 family protein 33C4 [Caenorhabditis elegans] ref|NP_503612.1| CYtochrome P450 family member (cyp-33C4) [Caenorhabditis elegans] 0.0
blast AAG24101 Cytochrome p450 family protein 33C5 [Caenorhabditis elegans] ref|NP_503616.1| CYtochrome P450 family member (cyp-33C5) [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-165
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-141
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.8E-96
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.6E-80
Sequence
Target Sequence 
>C. ELEGANS:189334_AT
tcggcaggactgtcgacaacctacacaacagttacctgggctcttgcctatgtcttgaat
tcgccggaagtattggagaagatgagatccgagttagatgaagttgttggaaaagataga
ttcgtgacaaccgctgataagaatgatctaccttatatgaacgcggctatcaatgaggtt
cagagatgtgcgaaccttttgccggtcaatgttgctcacgcaacaaccagggataccgtt
atcaatgggtatgcaattaaaaaggggactggagttattgcacagatcagtacagtgatg
cttgatgagaaggtcttcccggatccctataaattcaaccctgacagatttatagatgag
catgggaaacttatcaaagtcgagcagctgatcccattctcaatcggaagacggcagtgc
ccaggagaaggccttgctcggatggaaattttcctttttgtagcgaacttctttaatcat
tatcagatctccccatcatcggaagggttcccgtccatcgacaaaagcgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGGCAGGACTGTCGACAACCTACA 472 607 910 Antisense
CACAACAGTTACCTGGGCTCTTGCC 403 193 933 Antisense
TTGCCTATGTCTTGAATTCGCCGGA 105 703 953 Antisense
CAGAGATGTGCGAACCTTTTGCCGG 256 205 1090 Antisense
TTTGCCGGTCAATGTTGCTCACGCA 436 667 1107 Antisense
GCTCACGCAACAACCAGGGATACCG 437 301 1123 Antisense
TCTTCCCGGATCCCTATAAATTCAA 89 617 1223 Antisense
GGCAGTGCCCAGGAGAAGGCCTTGC 602 533 1322 Antisense
TAATCATTATCAGATCTCCCCATCA 578 635 1383 Antisense
CTCCCCATCATCGGAAGGGTTCCCG 236 229 1398 Antisense
GTTCCCGTCCATCGACAAAAGCGAC 18 443 1416 Antisense
Sequence Source Affymetrix Proprietary Database