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[189328_at] aldose reductase

Description ZC443.1 /REP_DB=WormBase Gene ID /WP=CE27788 /TR=Q23320 /GB=CAA99947.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=aldose reductase
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 44      4grp_highest_8_8 > 31      4grp_high_8_8 > 13      6grp_highest_nonorm > 81      6grp_total > 55      4grp_total > 11      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 369
1 2 3 4 range
A 234.5
(-1.6)
P 		238.1
(-1.5)
P 		163.6
(-3)
P 		470.2
(3)
P 		307
B 212.6
(-1.3)
P 		223.5
(-1.2)
P 		109.8
(-3)
P 		479.1
(3)
P 		369
C 187.8
(-3)
P 		248
(-1.6)
P 		186.7
(-3)
P 		448.8
(3)
P 		262
avr 211.6
(-1.9) 		236.5
(-1.4) 		153.4
(-3) 		466
(3) 		313

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 174
1 2 3 4 5 6 range
A 189.6
(-3)
P 		343.3
(2.3)
P 		304.7
(1)
P 		305.7
(1)
P 		247.8
(-1)
P 		363.9
(3)
P 		174
B 232.7
(-2.4)
P 		312.2
(1.9)
P 		333.5
(3)
P 		221.8
(-3)
P 		284.6
(0.4)
P 		324.2
(2.5)
P 		112
C 234.5
(-0.9)
P 		297.2
(3)
P 		272.9
(1.5)
P 		220.9
(-1.8)
P 		201.8
(-3)
P 		229.9
(-1.2)
P 		95
avr 218.9
(-3) 		317.6
(3) 		303.7
(2.2) 		249.5
(-1.1) 		244.7
(-1.4) 		306
(2.3) 		99
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 85.4
(3)
P 		51.7
(-1.7)
P 		42.8
(-3)
P 		68.8
(0.7)
P 		43
B 67.8
(0.3)
P 		55.2
(-0.7)
P 		25.5
(-3)
P 		102.1
(3)
P 		77
C 62.2
(-3)
P 		71.3
(1)
P 		65.1
(-1.7)
P 		75.7
(3)
P 		14
avr 71.8
(1.3) 		59.4
(-0.6) 		44.5
(-3) 		82.2
(3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 35
(-3)
P 		122.1
(3)
P 		66.3
(-0.8)
P 		77.5
(-0.1)
P 		74.8
(-0.3)
P 		88.6
(0.7)
P 		87
B 81
(-0.9)
P 		103.9
(1.4)
P 		81
(-0.9)
P 		58.8
(-3)
P 		115.6
(2.5)
P 		120.9
(3)
P 		62
C 86.9
(2.5)
P 		90.7
(3)
P 		69.5
(0.3)
P 		44.4
(-3)
P 		65.3
(-0.3)
P 		77
(1.2)
P 		46
avr 67.6
(-2) 		105.6
(3) 		72.3
(-1.4) 		60.2
(-3) 		85.2
(0.3) 		95.5
(1.7) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189328_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14665
Sequence Type Exemplar sequence
Representative Public ID ZC443.1 NCBI
Target Description ZC443.1 /REP_DB=WormBase Gene ID /WP=CE27788 /TR=Q23320 /GB=CAA99947.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=aldose reductase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073804(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073804 NCBI Caenorhabditis elegans ZC443.1 (ZC443.1) mRNA, complete cds. 10/11 A 
SNAP00000030942 ENSEMBL cdna:SNAP chromosome:CEL140:V:12811083:12812648:-1 10/11 None
GENEFINDER00000030950 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12811083:12812179:-1 10/11 None
ZC443.1 ENSEMBL cdna:known chromosome:CEL140:V:12811073:12812661:-1 gene:ZC443.1 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12811060-12812648(-) 100.0 100.0
Public Domain and Genome References
Gene Title aldose reductase
Ensembl ZC443.1
Entrez Gene 179756 Entrez gene
SwissProt Q23320 EMBL-EBI
WormBase CE27788 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1640451_AT dm
DROSGENOME1:142257_AT dm
Protein Similarities
Method ID Description E-Value
blast CAA99947 Hypothetical protein ZC443.1 [Caenorhabditis elegans] ref|NP_506205.1| ZC443.1 [Caenorhabditis elegans] 0.0
blast CAA99947 Hypothetical protein ZC443.1 [Caenorhabditis elegans] ref|NP_506205.1| ZC443.1 [Caenorhabditis elegans] 1.0E-168
blast CAE64002 Hypothetical protein CBG08596 [Caenorhabditis briggsae] 1.0E-157
blast CAE64002 Hypothetical protein CBG08596 [Caenorhabditis briggsae] 1.0E-144
blast CAE65877 Hypothetical protein CBG11027 [Caenorhabditis briggsae] 3.0E-85
Protein Domains
Database ID Description E-Value
Pfam IPR001395 EMBL-EBI Aldo/keto reductase 1.0E-126
Pfam IPR001395 EMBL-EBI Aldo/keto reductase 5.2E-115
Sequence
Target Sequence 
>C. ELEGANS:189328_AT
tgaaagtcagattgtccgaattatgaatatccaaaaagttcctattcacgcttctcagct
tgagcttcatctctatttgcctcaaaaagctcacagagagctatgcaaaaaacacaacat
cctcataacagcctatgcgacgttaggctctcctggaagaatgtctgtcgttggttcaaa
tggtagacctttgtttgaaagcactcaaaattctgaaaatgaaatgaacgacaaacatgt
gaaggcgctcgcacagaaatacagtaaaactccagctcagattcttctccgggctacagt
tgaaatgggaattatagttattccaaaaactacgaatcctgaaagaatgaaagaaaacat
caacatttttgatttcaacatttcaaatgctgaagtaaatctcctagaagcgcacgagag
aacaaaacaggaaagacttttctggtggccaaagtatttttttttaagcaacaaaaatga
tactgaacactatttttcagcgttgctga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAAGTCAGATTGTCCGAATTATG 124 571 480 Antisense
AAAAAGTTCCTATTCACGCTTCTCA 91 129 512 Antisense
TATTCACGCTTCTCAGCTTGAGCTT 527 663 522 Antisense
GCTTGAGCTTCATCTCTATTTGCCT 470 307 537 Antisense
CTATTTGCCTCAAAAAGCTCACAGA 139 225 552 Antisense
ACACAACATCCTCATAACAGCCTAT 592 111 591 Antisense
AGCCTATGCGACGTTAGGCTCTCCT 506 81 609 Antisense
CTCTCCTGGAAGAATGTCTGTCGTT 168 231 627 Antisense
GTCTGTCGTTGGTTCAAATGGTAGA 635 469 642 Antisense
GGTAGACCTTTGTTTGAAAGCACTC 44 505 661 Antisense
GAACACTATTTTTCAGCGTTGCTGA 99 353 964 Antisense
Sequence Source Affymetrix Proprietary Database