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[189317_at] tubulin alpha chain

Description ZK899.4 /REP_DB=WormBase Gene ID /WP=CE03837 /TR=SW:P52274 /GB=CAA85497.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=alpha-tubulin
Cluster 6grp_high1_10_10 > 60      6grp_total > 50      4grp_total > 58      6grp_cgc > 60      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1011
1 2 3 4 range
A 289.4
(-1)
P 		855
(3)
P 		783.5
(2.5)
P 		14.1
(-3)
A 		841
B 296.6
(-1.4)
P 		1025.2
(3)
P 		825.6
(1.8)
P 		34.2
(-3)
A 		991
C 323.2
(-1.2)
P 		916.1
(3)
P 		915.7
(3)
P 		65.5
(-3)
A 		851
avr 303.1
(-1.2) 		932.1
(3) 		841.6
(2.4) 		37.9
(-3) 		894

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1855
1 2 3 4 5 6 range
A 1331.9
(1.3)
P 		907.5
(-0.3)
P 		1218.9
(0.8)
P 		1816.9
(3)
P 		720.1
(-1)
P 		153.4
(-3)
P 		1664
B 1534.2
(2.5)
P 		851.6
(-0.2)
P 		1375.4
(1.9)
P 		1657
(3)
P 		808.5
(-0.3)
P 		132.3
(-3)
P 		1525
C 1416.8
(1.3)
P 		855.5
(-0.6)
P 		1666.9
(2.1)
P 		1955.2
(3)
P 		913.7
(-0.4)
P 		100.2
(-3)
A 		1855
avr 1427.6
(1.6) 		871.5
(-0.3) 		1420.4
(1.6) 		1809.7
(3) 		814.1
(-0.6) 		128.6
(-3) 		1681
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 317
1 2 3 4 range
A 105.4
(0.1)
P 		185.6
(2.4)
P 		204.8
(3)
P 		2.1
(-3)
A 		203
B 94.6
(-0.9)
P 		253.2
(3)
P 		191.3
(1.5)
P 		7.3
(-3)
A 		246
C 107
(-1.1)
P 		263.4
(1.9)
P 		319.1
(3)
P 		11
(-3)
A 		308
avr 102.3
(-0.5) 		234.1
(2.9) 		238.4
(3) 		6.8
(-3) 		232

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 500
1 2 3 4 5 6 range
A 245.5
(-0.1)
P 		322.7
(1)
P 		265.4
(0.2)
P 		460.8
(3)
P 		217.3
(-0.4)
P 		37.3
(-3)
P 		424
B 533.8
(3)
P 		283.5
(-0.1)
P 		334
(0.5)
P 		438.9
(1.8)
P 		328.5
(0.5)
P 		49.3
(-3)
P 		485
C 525.1
(3)
P 		261
(-0.2)
P 		424.3
(1.8)
P 		393.2
(1.4)
P 		295.7
(0.2)
P 		33.5
(-3)
A 		492
avr 434.8
(3) 		289.1
(0.8) 		341.2
(1.6) 		431
(2.9) 		280.5
(0.7) 		40
(-3) 		395
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189317_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16717
Sequence Type Exemplar sequence
Representative Public ID ZK899.4 NCBI
Target Description ZK899.4 /REP_DB=WormBase Gene ID /WP=CE03837 /TR=SW:P52274 /GB=CAA85497.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=alpha-tubulin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077190(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077190 NCBI Caenorhabditis elegans TuBulin, Alpha family member (tba-8) (tba-8) mRNA, complete cds. 11/11 None
SNAP00000025042 ENSEMBL cdna:SNAP chromosome:CEL140:X:9455492:9458145:-1 11/11 None
GENEFINDER00000025053 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9455492:9458665:-1 11/11 None
ZK899.4 ENSEMBL cdna:known chromosome:CEL140:X:9455447:9458187:-1 gene:ZK899.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9455491-9458080(-) 99.85 99.85
Public Domain and Genome References
Gene Title tubulin alpha chain
Gene Symbol tba-8
Ensembl ZK899.4
Entrez Gene 191455 Entrez gene
SwissProt P52274 EMBL-EBI
WormBase CE37468 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
46785 microtubule polymerization inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5874 microtubule inferred from electronic annotation QuickGO AmiGO
45298 tubulin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3924 GTPase activity inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA85497 Hypothetical protein ZK899.4 [Caenorhabditis elegans] ref|NP_509591.2| TuBulin, Alpha family member (tba-8) [Caenorhabditis elegans] sp|P52274|TBA8_CAEEL Tubulin alpha-8 chain 0.0
blast CAE69815 Hypothetical protein CBG16131 [Caenorhabditis briggsae] 0.0
blast NP_989078 tubulin, alpha 7 [Xenopus tropicalis] gb|AAH61260.1| Hypothetical protein MGC75684 [Xenopus tropicalis] gb|AAH46841.1| Tuba6-prov protein [Xenopus laevis] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 4.7E-103
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 5.9E-72
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 7.5E-99
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 5.9E-72
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 7.5E-99
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 5.9E-72
Sequence
Target Sequence 
>C. ELEGANS:189317_AT
gacaaacctggttccatatccaagaattcattttccactggctacctacgctccagtcat
ttcagctgatagggctcaccacgaacccttgtcagttcaagatatcactcacatgtgttt
cgaaaaggctaatcagatggtgaaatgtgacccaagtgctggaaaatacatggcggtctg
tttgctttatcgcggcgacgtcgttcctaaggatgtcaacgcagctatttcgtcagtaaa
ggcaaaacgaggaatcaactttgtagattggtgtccaactggattcaaagtcggaatcaa
ctaccaagccccaataacagtcgagggtggagatcttgcaaaggtacaccgtgctgtttg
catgctgtccaataccacagcaattgctgaagcgtgggctcgtctcgaccacaaatttga
cctaatgtattcgaagcgcgcatttgtgcactggtttgtaggagaaggtatggaggaagg
agaattcatggaagctcgtgatgatctggctgctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAAACCTGGTTCCATATCCAAGA 652 299 777 Antisense
AGAATTCATTTTCCACTGGCTACCT 37 75 799 Antisense
TTCAGCTGATAGGGCTCACCACGAA 274 687 837 Antisense
TCACCACGAACCCTTGTCAGTTCAA 674 625 852 Antisense
TACATGGCGGTCTGTTTGCTTTATC 631 643 943 Antisense
GGATGTCAACGCAGCTATTTCGTCA 90 515 987 Antisense
CTACCAAGCCCCAATAACAGTCGAG 169 225 1077 Antisense
AAAGGTACACCGTGCTGTTTGCATG 653 121 1116 Antisense
TGTTTGCATGCTGTCCAATACCACA 389 561 1131 Antisense
AGCGCGCATTTGTGCACTGGTTTGT 218 83 1211 Antisense
GAAGCTCGTGATGATCTGGCTGCTC 407 343 1267 Antisense
Sequence Source Affymetrix Proprietary Database