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[189316_at]

Description T27A10.3 /REP_DB=WormBase Gene ID /WP=CE07553 /TR=Q22820 /GB=AAB52502.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=choline kinase
Cluster 6grp_high1_10_10 > 40      6grp_total > 41      4grp_total > 61      6grp_cgc > 82      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 439
1 2 3 4 range
A 508.1
(2.5)
P 		465.5
(1.8)
P 		543.1
(3)
P 		141.3
(-3)
A 		402
B 518.5
(3)
P 		388.4
(0.9)
P 		515.1
(2.9)
P 		145.4
(-3)
A 		373
C 579.8
(3)
P 		420.6
(0.4)
P 		568.2
(2.8)
P 		216
(-3)
M 		364
avr 535.5
(2.9) 		424.8
(1.1) 		542.1
(3) 		167.6
(-3) 		375

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 551
1 2 3 4 5 6 range
A 382
(-1.1)
A 		410.8
(-0.8)
P 		756.1
(3)
P 		478.2
(-0)
P 		210.8
(-2.9)
P 		205.3
(-3)
P 		551
B 490.9
(1.4)
P 		502.7
(1.7)
P 		570
(3)
P 		456.1
(0.7)
P 		357.1
(-1.3)
P 		270.6
(-3)
P 		299
C 394.1
(-0.8)
P 		531.2
(1.6)
P 		609.6
(3)
P 		607.9
(3)
P 		273.4
(-3)
P 		287.7
(-2.7)
P 		336
avr 422.3
(-0.4) 		481.6
(0.5) 		645.2
(3) 		514.1
(1) 		280.4
(-2.6) 		254.5
(-3) 		391
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 185
(3)
P 		101
(-0.1)
P 		142
(1.4)
P 		20.7
(-3)
A 		164
B 165.4
(3)
P 		95.9
(-0.1)
P 		119.4
(0.9)
P 		31
(-3)
A 		134
C 191.9
(2.8)
P 		120.9
(0.1)
P 		198
(3)
P 		36.4
(-3)
M 		162
avr 180.8
(3) 		105.9
(0) 		153.1
(1.9) 		29.4
(-3) 		151

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 70.4
(-1.9)
A 		146.1
(2)
P 		164.7
(3)
P 		121.3
(0.7)
P 		63.6
(-2.3)
P 		50
(-3)
P 		115
B 170.8
(3)
P 		167.3
(2.7)
P 		138.4
(0.2)
P 		120.8
(-1.3)
P 		145.1
(0.8)
P 		100.9
(-3)
P 		70
C 146.1
(1.7)
P 		162.1
(3)
P 		155.2
(2.4)
P 		122.3
(-0.2)
P 		88.5
(-3)
P 		96.3
(-2.4)
P 		74
avr 129.1
(0.7) 		158.5
(3) 		152.8
(2.6) 		121.5
(0.1) 		99.1
(-1.7) 		82.4
(-3) 		76
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189316_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18233
Sequence Type Exemplar sequence
Representative Public ID T27A10.3 NCBI
Target Description T27A10.3 /REP_DB=WormBase Gene ID /WP=CE07553 /TR=Q22820 /GB=AAB52502.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=choline kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029758(11), NM_001029759(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029758 NCBI Caenorhabditis elegans Choline Kinase C family member (ckc-1) (ckc-1) mRNA, complete cds. 11/11 None
NM_001029759 NCBI Caenorhabditis elegans Choline Kinase C family member (ckc-1) (ckc-1) mRNA, complete cds. 10/11 None
SNAP00000016181 ENSEMBL cdna:SNAP chromosome:CEL140:X:3576601:3578713:1 11/11 None
GENEFINDER00000016186 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3576601:3578713:1 11/11 None
T27A10.3a.1 ENSEMBL cdna:known chromosome:CEL140:X:3576593:3578800:1 gene:T27A10.3 11/11 None
T27A10.3a.2 ENSEMBL cdna:known chromosome:CEL140:X:3576595:3578713:1 gene:T27A10.3 11/11 None
T27A10.3b ENSEMBL cdna:known chromosome:CEL140:X:3576601:3578316:1 gene:T27A10.3 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3576601-3578714(+) 100.0 100.0
Public Domain and Genome References
Ensembl T27A10.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB52502 Choline kinase c protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024929.1| Choline Kinase C family member (ckc-1) [Caenorhabditis elegans] 0.0
blast AAO91673 Choline kinase c protein 1, isoform b [Caenorhabditis elegans] ref|NP_001024930.1| Choline Kinase C family member (ckc-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 1.0E-126
Pfam IPR002573 EMBL-EBI Choline/ethanolamine kinase 5.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189316_AT
cactcttccgttccaaccaatggaaaaactccagtgtttgaaaaaatgcgaacattttta
caacagctgaatccgtcatttgaaaaagaaagccaacagaattttttccacgagaacttt
ccaactgatctaggagcagaaatttccaaaattgaaaagatgatcgtaatgctaaaagag
ccaattgtattctgtcataacgaccttcttgtgcacaacattgtgtatgacagtgagaag
aaatcaatcgaatttattgactatgaatatgcattcccaaattatgctctatacgacatt
gccaatcacttttgtgaatatgcaggcgtcgaaggatctcctgactactcaaaatgtttg
acaaaagacgagaagtgggcatttatcaacgactatttacgtttttcaaatggaaaagag
cactctgacacccgaattgctacaatgttcaagaacttgctattgtttgaagcagccgcc
catcttttctgggctgtgtgggcacttgtccaagcacagaattcaacgattgactttgat
tatttaacgtacgctcacgctcgatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACTCTTCCGTTCCAACCAATGGAA 627 197 424 Antisense
ACAACAGCTGAATCCGTCATTTGAA 399 111 483 Antisense
TTCCACGAGAACTTTCCAACTGATC 604 697 529 Antisense
GTCATAACGACCTTCTTGTGCACAA 558 467 617 Antisense
TGCTCTATACGACATTGCCAATCAC 45 581 708 Antisense
AGGCGTCGAAGGATCTCCTGACTAC 159 53 747 Antisense
GGAAAAGAGCACTCTGACACCCGAA 315 527 835 Antisense
TGCTATTGTTTGAAGCAGCCGCCCA 396 577 881 Antisense
CCGCCCATCTTTTCTGGGCTGTGTG 347 263 899 Antisense
CTGTGTGGGCACTTGTCCAAGCACA 615 239 917 Antisense
TTTAACGTACGCTCACGCTCGATAT 448 663 966 Antisense
Sequence Source Affymetrix Proprietary Database