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[189314_at] cytochrome P450

Description C45H4.2 /REP_DB=WormBase Gene ID /WP=CE08736 /TR=O44704 /GB=AAC25869.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_total > 80      4grp_total > 30      6grp_cgc > 86      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 6.7
(-2.5)
A 		8.8
(-0.6)
A 		6.2
(-3)
A 		12.7
(3)
A 		7
B 6.7
(3)
A 		4
(-1.8)
A 		3.8
(-2.1)
A 		3.3
(-3)
A 		3
C 10.8
(3)
A 		5.3
(-1.5)
A 		3.4
(-3)
A 		3.8
(-2.7)
A 		7
avr 8.1
(3) 		6
(-0.5) 		4.5
(-3) 		6.6
(0.5) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 5.3
(-2.8)
A 		4.7
(-2.9)
A 		10.6
(-1.8)
A 		36.4
(3)
A 		13.5
(-1.3)
A 		4.3
(-3)
A 		32
B 3.5
(-3)
A 		7.4
(-2.3)
A 		3.9
(-2.9)
A 		37.3
(3)
M 		15.5
(-0.9)
A 		4.6
(-2.8)
A 		34
C 8.9
(-1.7)
A 		3.7
(-3)
A 		24
(2.3)
A 		20.7
(1.4)
A 		26.9
(3)
A 		21.1
(1.5)
A 		23
avr 5.9
(-2.9) 		5.3
(-3) 		12.8
(-1.3) 		31.5
(3) 		18.6
(0) 		10
(-1.9) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3
1 2 3 4 range
A 2.4
(3)
A 		1.9
(-0.8)
A 		1.6
(-3)
A 		1.9
(-0.8)
A 		1
B 2.1
(3)
A 		1
(-1.7)
A 		0.9
(-2.1)
A 		0.7
(-3)
A 		1
C 3.6
(3)
A 		1.5
(-1.2)
A 		1.2
(-1.8)
A 		0.6
(-3)
A 		3
avr 2.7
(3) 		1.5
(-1.5) 		1.2
(-2.6) 		1.1
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 1
(-3)
A 		1.7
(-2.5)
A 		2.3
(-2)
A 		9.2
(3)
A 		4.1
(-0.7)
A 		1
(-3)
A 		8
B 1.2
(-2.8)
A 		2.5
(-1.9)
A 		0.9
(-3)
A 		9.9
(3)
M 		6.3
(0.6)
A 		1.7
(-2.5)
A 		9
C 3.3
(-1.3)
A 		1.1
(-3)
A 		6.1
(0.9)
A 		4.2
(-0.6)
A 		8.7
(3)
A 		7.1
(1.7)
A 		8
avr 1.8
(-3) 		1.8
(-3) 		3.1
(-1.7) 		7.8
(3) 		6.4
(1.6) 		3.3
(-1.5) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189314_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14857
Sequence Type Exemplar sequence
Representative Public ID C45H4.2 NCBI
Target Description C45H4.2 /REP_DB=WormBase Gene ID /WP=CE08736 /TR=O44704 /GB=AAC25869.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071191(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071191 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-33C1) (cyp-33C1) mRNA, complete cds. 11/11 None
SNAP00000009348 ENSEMBL cdna:SNAP chromosome:CEL140:V:2179414:2181587:1 11/11 None
GENEFINDER00000009360 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2180055:2181587:1 11/11 None
C45H4.2 ENSEMBL cdna:known chromosome:CEL140:V:2179414:2181587:1 gene:C45H4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2179413-2181587(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-33C1
Ensembl C45H4.2
Entrez Gene 183484 Entrez gene
SwissProt O44704 EMBL-EBI
WormBase CE08736 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16172_AT cytochrome P450 family protein at
ATH1-121501:257635_AT cytochrome P450 family protein at
RAE230B:1396327_AT similar to cytochrome P450, family 2, subfamily J, polypeptide 4 rn
RAT230_2:1396327_AT similar to cytochrome P450, family 2, subfamily J, polypeptide 4 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC25869 Cytochrome p450 family protein 33C1 [Caenorhabditis elegans] ref|NP_503592.1| CYtochrome P450 family member (cyp-33C1) [Caenorhabditis elegans] 0.0
blast AAC25881 Cytochrome p450 family protein 33C2 [Caenorhabditis elegans] ref|NP_503594.1| CYtochrome P450 family member (cyp-33C2) [Caenorhabditis elegans] 0.0
blast CAE64212 Hypothetical protein CBG08845 [Caenorhabditis briggsae] 0.0
blast CAE74164 Hypothetical protein CBG21837 [Caenorhabditis briggsae] emb|CAE74152.1| Hypothetical protein CBG21823 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-161
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-162
p450 CP1B_HUMAN Animalia; CYP1B1; hum; CP1B_HUMAN; Homo sapiens; Q16678 1.0E-127
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 8.5E-108
Pfam IPR001128 EMBL-EBI Cytochrome P450 9.2E-108
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.1E-9
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.0E-23
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.3E-45
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503592.1 1 2-20
C45H4.2 1 2-20
SNAP00000009348 1 2-20
Sequence
Target Sequence 
>C. ELEGANS:189314_AT
gcattgatctttggtttgcgggcctcaacaccacaacaaacactataacatgggcaatca
gctacgttttgcatcatccagaggttcaggataagattcatgaggaactagacaaagtga
ttggaagtgataggcttatcaccacagctgataagaatgatcttccgtacttcaacgcgt
ctataaatgaatcacaacgaggtatcaacattctccccctgaacttgcagcacgcaacca
cccgtgatactgtgatagatgggttcaaaatccccaagggcactggagtggttgcccaaa
ttagtaccgttatgaataatgaggaagtgttcccagatccgtacacttttaatccagaca
gatttatcgatgaaaatgggaaattgaagaaggtagacgagctcgccccgttctcagtcg
gaaaacgaagttgcccgggagaaggccttgctcggatggagcttttccttttcattgcta
attttttgaatagatacaagatacatccctcaaaagaaggcctaccgtcaatggccaaag
gcagcggaccagtggttgctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCATTGATCTTTGGTTTGCGGGCCT 303 307 908 Antisense
CCTCAACACCACAACAAACACTATA 285 267 930 Antisense
CAACGAGGTATCAACATTCTCCCCC 3 189 1102 Antisense
AAATCCCCAAGGGCACTGGAGTGGT 169 111 1175 Antisense
GTTGCCCAAATTAGTACCGTTATGA 622 437 1198 Antisense
TGAGGAAGTGTTCCCAGATCCGTAC 529 567 1227 Antisense
AGATCCGTACACTTTTAATCCAGAC 333 65 1242 Antisense
TGAAGAAGGTAGACGAGCTCGCCCC 272 573 1292 Antisense
ACATCCCTCAAAAGAAGGCCTACCG 581 103 1410 Antisense
AGGCCTACCGTCAATGGCCAAAGGC 407 45 1425 Antisense
AAAGGCAGCGGACCAGTGGTTGCTC 418 121 1444 Antisense
Sequence Source Affymetrix Proprietary Database