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[189313_at] ACHR

Description K03F8.2 /REP_DB=WormBase Gene ID /WP=CE01357 /GEN=acr-5 /TR=Q21195 /GB=AAA50714.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=ACHR
Cluster 6grp_total > 43      4grp_total > 04      6grp_cgc > 90      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 19.1
(-2.5)
A 		31.1
(-1.6)
A 		12.9
(-3)
A 		91.3
(3)
M 		78
B 40.9
(1.5)
A 		19.6
(-3)
A 		21
(-2.7)
A 		48.1
(3)
A 		29
C 24.4
(-1.7)
A 		7.4
(-3)
A 		10.6
(-2.8)
A 		88.5
(3)
P 		81
avr 28.1
(-1.7) 		19.4
(-2.5) 		14.8
(-3) 		76
(3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 27.2
(0.3)
A 		1.9
(-3)
A 		47.6
(3)
A 		5.7
(-2.5)
A 		36.9
(1.6)
A 		11.7
(-1.7)
A 		46
B 40
(2.5)
A 		24.4
(-0)
A 		43.2
(3)
P 		6.2
(-3)
A 		26.6
(0.3)
A 		22.6
(-0.3)
A 		37
C 28.3
(1.3)
A 		7.8
(-2.7)
A 		6.3
(-3)
A 		37.2
(3)
A 		12.2
(-1.9)
A 		9.5
(-2.4)
A 		31
avr 31.8
(2.8) 		11.4
(-3) 		32.4
(3) 		16.4
(-1.6) 		25.2
(0.9) 		14.6
(-2.1) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 6.9
(-0.9)
A 		6.7
(-1)
A 		3.4
(-3)
A 		13.4
(3)
M 		10
B 13
(3)
A 		4.8
(-3)
A 		4.9
(-2.9)
A 		10.3
(1)
A 		8
C 8.1
(-0.2)
A 		2.1
(-3)
A 		3.7
(-2.3)
A 		14.9
(3)
P 		13
avr 9.3
(0.6) 		4.5
(-2.7) 		4
(-3) 		12.9
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 5
(-0.5)
A 		0.7
(-3)
A 		10.4
(2.6)
A 		1.4
(-2.6)
A 		11.1
(3)
A 		2.8
(-1.8)
A 		10
B 13.9
(3)
A 		8.1
(0.2)
A 		10.5
(1.3)
P 		1.6
(-3)
A 		10.8
(1.5)
A 		8.4
(0.3)
A 		12
C 10.5
(3)
A 		2.4
(-2.5)
A 		1.6
(-3)
A 		7.5
(1)
A 		4
(-1.4)
A 		3.2
(-1.9)
A 		9
avr 9.8
(3) 		3.7
(-2.8) 		7.5
(0.8) 		3.5
(-3) 		8.6
(1.9) 		4.8
(-1.8) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189313_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7492
Sequence Type Exemplar sequence
Representative Public ID K03F8.2 NCBI
Target Description K03F8.2 /REP_DB=WormBase Gene ID /WP=CE01357 /GEN=acr-5 /TR=Q21195 /GB=AAA50714.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=ACHR
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066036(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066036 NCBI Caenorhabditis elegans K03F8.2 (ACHR) mRNA, complete cds. 11/11 None
GENEFINDER00000006523 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6510539:6514700:-1 9/11 None
K03F8.2 ENSEMBL cdna:known chromosome:CEL140:III:6510891:6514700:-1 gene:K03F8.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000006522 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6510889-6514699(-) 100.0 100.0
Public Domain and Genome References
Gene Title ACHR
Gene Symbol acr-5
Ensembl K03F8.2
Entrez Gene 191596 Entrez gene
WormBase CE38967 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498437 K03F8.2 [Caenorhabditis elegans] sp|P54246|ACR5_CAEEL Acetylcholine receptor, alpha-type subunit acr-5 precursor 0.0
blast AAZ94293 Acetylcholine receptor protein 5 [Caenorhabditis elegans] 0.0
blast T16546 hypothetical protein K03F8.2 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 1.1E-5
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 1.1E-67
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498437.1 3 322-344,357-375,390-412
K03F8.2 3 322-344,357-375,390-412
GENEFINDER00000006523 3 322-344,357-375,390-412
Sequence
Target Sequence 
>C. ELEGANS:189313_AT
aatgctgtacggcaccatgggtgatgctatatgcacatttagtaattagaagaaaaccat
tgtactatatgattaatttggttgttcctacttccattattacaatagtagcagtaactg
gattctttacaccaacgtcgtcatcaagtgaaagggatgagaaattatatcttggtatca
atactcttctgacaatgtcagttatgatgttaatggtttgcaatcaaatgccttctacaa
gcacatatgttccattgatgagttggtattatattggaataattatggtgattgttgttg
gaacatttctagcaacaggagttctggcaattcatggacaaaagcattataacaaaccga
tttctgacaggatacgaaaattgatatataatccggttgttgaatttttcatattaagtc
ctccaacatctttaatcgatttatggacagaatttggagtgatatccgaacagcgacact
ctacacatctagacccacttcttttgcaacatatggatcctatttcagtaagtcttttgc
cttttcctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGCTGTACGGCACCATGGGTGAT 275 165 833 Antisense
ATTTGGTTGTTCCTACTTCCATTAT 220 19 908 Antisense
TGGATTCTTTACACCAACGTCGTCA 401 549 951 Antisense
GTATCAATACTCTTCTGACAATGTC 547 453 1007 Antisense
GCAATCAAATGCCTTCTACAAGCAC 296 323 1052 Antisense
GGAGTTCTGGCAATTCATGGACAAA 603 517 1150 Antisense
AAGTCCTCCAACATCTTTAATCGAT 356 155 1248 Antisense
TGATATCCGAACAGCGACACTCTAC 640 563 1292 Antisense
TAGACCCACTTCTTTTGCAACATAT 346 653 1322 Antisense
GCAACATATGGATCCTATTTCAGTA 367 319 1338 Antisense
TCAGTAAGTCTTTTGCCTTTTCCTT 549 619 1357 Antisense
Sequence Source Affymetrix Proprietary Database