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[189311_s_at]

Description H19M22.2 /REP_DB=WormBase Gene ID /WP=CE26469 /CHR=3 /FEA=Sanger Annotation /DEF=locus:let-805 (ST.LOUIS) TR:O44838 protein_id:AAB94998.1
Cluster 6grp_total > 21      4grp_total > 52      6grp_cgc > 44      6grp_cgc_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 100.7
(-0.6)
P 		177.1
(3)
P 		118.9
(0.3)
P 		49.2
(-3)
A 		128
B 126.4
(3)
P 		95.2
(1)
P 		85
(0.3)
P 		34.3
(-3)
A 		92
C 112.1
(0.5)
P 		141.7
(3)
P 		107.4
(0.1)
P 		69.7
(-3)
A 		72
avr 113.1
(1.3) 		138
(3) 		103.8
(0.6) 		51.1
(-3) 		87

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 104
1 2 3 4 5 6 range
A 24.4
(-2.2)
A 		107.5
(3)
P 		49.8
(-0.6)
A 		34.7
(-1.5)
A 		11.1
(-3)
A 		57.2
(-0.1)
A 		96
B 19.4
(-2.2)
A 		77.6
(3)
A 		59.9
(1.4)
A 		15.6
(-2.5)
A 		10.2
(-3)
A 		34.1
(-0.9)
A 		67
C 91.1
(1.7)
P 		40.1
(-1.2)
A 		112.8
(3)
M 		28.1
(-1.9)
A 		84.8
(1.4)
P 		8.7
(-3)
A 		104
avr 45
(-0.7) 		75.1
(3) 		74.2
(2.9) 		26.1
(-3) 		35.4
(-1.9) 		33.3
(-2.1) 		49
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 36.7
(2.7)
P 		38.4
(3)
P 		31.1
(1.6)
P 		7.2
(-3)
A 		31
B 40.3
(3)
P 		23.5
(-0.1)
P 		19.7
(-0.7)
P 		7.3
(-3)
A 		33
C 37.1
(2.3)
P 		40.7
(3)
P 		37.4
(2.3)
P 		11.8
(-3)
A 		29
avr 38
(3) 		34.2
(2.2) 		29.4
(1.2) 		8.8
(-3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 4.5
(-2.8)
A 		38.2
(3)
P 		10.8
(-1.7)
A 		8.8
(-2.1)
A 		3.4
(-3)
A 		13.9
(-1.2)
A 		35
B 6.7
(-2.3)
A 		25.8
(3)
A 		14.5
(-0.1)
A 		4.1
(-3)
A 		4.1
(-3)
A 		12.7
(-0.6)
A 		22
C 33.8
(3)
P 		12.2
(-1.2)
A 		28.7
(2)
M 		5.6
(-2.5)
A 		27.4
(1.8)
P 		2.9
(-3)
A 		31
avr 15
(-0.3) 		25.4
(3) 		18
(0.7) 		6.2
(-3) 		11.6
(-1.3) 		9.8
(-1.9) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189311_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7553
Sequence Type Exemplar sequence
Representative Public ID H19M22.2 NCBI
Target Description H19M22.2 /REP_DB=WormBase Gene ID /WP=CE26469 /CHR=3 /FEA=Sanger Annotation /DEF=locus:let-805 (ST.LOUIS) TR:O44838 protein_id:AAB94998.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027469(11), NM_001027470(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027469 NCBI Caenorhabditis elegans LEThal family member (let-805) (let-805) mRNA, complete cds. 11/11 None
NM_001027470 NCBI Caenorhabditis elegans LEThal family member (let-805) (let-805) mRNA, complete cds. 11/11 None
H19M22.2b.2 ENSEMBL cdna:known chromosome:CEL140:III:2658071:2681093:1 gene:H19M22.2 11/11 None
H19M22.2a ENSEMBL cdna:known chromosome:CEL140:III:2658073:2681101:1 gene:H19M22.2 11/11 A 
H19M22.2b.1 ENSEMBL cdna:known chromosome:CEL140:III:2658073:2681101:1 gene:H19M22.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175384 4 2 NM_001027469 175691_s_at (11), 189311_s_at (11)
NM_001027470 175691_s_at (11), 177186_s_at (11), 189311_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2658085-2670256(+) 100.0 100.0
Public Domain and Genome References
Ensembl H19M22.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK21414 Lethal protein 805, isoform a [Caenorhabditis elegans] ref|NP_001022640.1| LEThal family member (let-805) [Caenorhabditis elegans] gb|AAD37411.1| myotactin form A [Caenorhabditis elegans] 0.0
blast AAK21413 Lethal protein 805, isoform b [Caenorhabditis elegans] ref|NP_001022641.1| LEThal family member (let-805) [Caenorhabditis elegans] gb|AAD37410.1| myotactin form B [Caenorhabditis elegans] 0.0
blast AAO91708 Lethal protein 805, isoform d [Caenorhabditis elegans] ref|NP_001022643.1| LEThal family member (let-805) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003961 EMBL-EBI Fibronectin, type III 4.6E-5
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.9E-9
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.6E-10
Pfam IPR003961 EMBL-EBI Fibronectin, type III 4.9E-10
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.9E-6
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.4E-22
Pfam IPR003961 EMBL-EBI Fibronectin, type III 8.6E-17
Pfam IPR003961 EMBL-EBI Fibronectin, type III 5.9E-6
Pfam IPR003961 EMBL-EBI Fibronectin, type III 9.8E-14
Pfam IPR003961 EMBL-EBI Fibronectin, type III 6.5E-5
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.0E-9
Pfam IPR003961 EMBL-EBI Fibronectin, type III 4.0E-15
Pfam IPR003961 EMBL-EBI Fibronectin, type III 4.0E-15
Pfam IPR003961 EMBL-EBI Fibronectin, type III 9.5E-10
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.9E-16
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.6E-6
Pfam IPR003961 EMBL-EBI Fibronectin, type III 3.4E-7
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.3E-20
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.8E-18
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.4E-12
Pfam IPR003961 EMBL-EBI Fibronectin, type III 5.2E-8
Pfam IPR003961 EMBL-EBI Fibronectin, type III 3.1E-14
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.5E-13
Pfam IPR003961 EMBL-EBI Fibronectin, type III 3.4E-6
Pfam IPR003961 EMBL-EBI Fibronectin, type III 7.0E-10
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.0E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022640.1 2 7-29,3909-3931
NP_001022641.1 2 7-29,3906-3928
H19M22.2b.2 2 7-29,3906-3928
H19M22.2a 2 7-29,3909-3931
H19M22.2b.1 2 7-29,3906-3928
Sequence
Target Sequence 
>C. ELEGANS:189311_S_AT
aatacttcggatcttcgtgtcactatcgatggattgaccccatacaccaagtatgtgatg
agagttaaggcctacaactcgattggtggaggaccaaacactgagaatctcgttgtcatg
accgccaaggctcaagccccacttccaccacaggatcttgtagttgctcaagaaggaacc
agcttcttcatggtatcctggcttccaccatacccaccatacggcccacacgatgcttac
aagatccgctatcaacagatcccatcggacgattggaaggaggtggagaaaggaatcaag
gatccacttcttcagtgcccaggagaaagtccgagattctgttacaacgcaactggattg
gattcagggcaacagttcaagattcaagttgcgacaagaattgagggaggaagctacgga
ccatggtcatcgttggtgattgctaacacgcttcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATACTTCGGATCTTCGTGTCACTA 185 173 6250 Antisense
GATGGATTGACCCCATACACCAAGT 642 407 6277 Antisense
TAAGGCCTACAACTCGATTGGTGGA 108 635 6315 Antisense
TGAGAATCTCGTTGTCATGACCGCC 483 569 6351 Antisense
TGTCATGACCGCCAAGGCTCAAGCC 187 559 6363 Antisense
CCACTTCCACCACAGGATCTTGTAG 494 273 6388 Antisense
CAGCTTCTTCATGGTATCCTGGCTT 46 205 6429 Antisense
GGCCCACACGATGCTTACAAGATCC 132 543 6472 Antisense
AGATCCGCTATCAACAGATCCCATC 294 67 6491 Antisense
GGAGGAAGCTACGGACCATGGTCAT 541 515 6655 Antisense
GTTGGTGATTGCTAACACGCTTCAA 484 435 6681 Antisense
Sequence Source Affymetrix Proprietary Database