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[189302_s_at] vacuolar ATPase G subunit

Description F46F11.5 /REP_DB=WormBase Gene ID /WP=CE10604 /GB=AAK21386.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=vacuolar ATPase G subunit
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 12      4grp_highest_8_8 > 24      4grp_high_8_8 > 52      6grp_highest_nonorm > 05      6grp_total > 23      4grp_total > 59      6grp_highest_sub > 01_02_03      6grp_cgc > 71      6grp_high1_sub > De      6grp_cgc_sub > 03      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5218
1 2 3 4 range
A 7536.3
(2.2)
P 		8222.6
(3)
P 		8130.1
(2.9)
P 		3241
(-3)
P 		4982
B 7252.8
(1.5)
P 		8459.3
(3)
P 		8024.7
(2.5)
P 		3658.5
(-3)
P 		4801
C 7508.9
(2.3)
P 		8022.9
(3)
P 		8039.3
(3)
P 		3448.8
(-3)
P 		4591
avr 7432.7
(2) 		8234.9
(3) 		8064.7
(2.8) 		3449.4
(-3) 		4786

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4908
1 2 3 4 5 6 range
A 7452.2
(2.3)
P 		7299.3
(2.1)
P 		8017.9
(3)
P 		5753.8
(0.2)
P 		3645.9
(-2.3)
P 		3109.8
(-3)
P 		4908
B 7702
(3)
P 		7193.7
(2.3)
P 		7393.6
(2.6)
P 		4895
(-0.7)
P 		3866.1
(-2)
P 		3142.9
(-3)
P 		4559
C 6972.5
(2.2)
P 		7522
(3)
P 		6596.6
(1.6)
P 		5009.3
(-0.7)
P 		3832
(-2.4)
P 		3423.1
(-3)
P 		4099
avr 7375.6
(3) 		7338.3
(2.9) 		7336
(2.9) 		5219.4
(-0.1) 		3781.3
(-2.2) 		3225.3
(-3) 		4150
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2328
1 2 3 4 range
A 2744
(3)
P 		1784.9
(0.5)
P 		2125.4
(1.4)
P 		474.4
(-3)
P 		2270
B 2312.9
(3)
P 		2089.2
(2.1)
P 		1859.5
(1.2)
P 		779.6
(-3)
P 		1533
C 2485.7
(2.1)
P 		2306.6
(1.7)
P 		2801.9
(3)
P 		581.5
(-3)
P 		2220
avr 2514.2
(3) 		2060.2
(1.6) 		2262.3
(2.2) 		611.8
(-3) 		1902

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1923
1 2 3 4 5 6 range
A 1373.7
(-1)
P 		2596
(3)
P 		1746.1
(0.2)
P 		1459.3
(-0.7)
P 		1100.2
(-1.9)
P 		757.1
(-3)
P 		1839
B 2679.9
(3)
P 		2394.5
(1.9)
P 		1795.4
(-0.5)
P 		1296.5
(-2.5)
P 		1570.7
(-1.4)
P 		1172
(-3)
P 		1508
C 2584.4
(3)
P 		2295.2
(1.9)
P 		1679
(-0.4)
P 		1007.4
(-3)
P 		1240
(-2.1)
P 		1145.9
(-2.5)
P 		1577
avr 2212.7
(2.1) 		2428.6
(3) 		1740.2
(0.1) 		1254.4
(-2) 		1303.6
(-1.8) 		1025
(-3) 		1404
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189302_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2341
Sequence Type Exemplar sequence
Representative Public ID F46F11.5 NCBI
Target Description F46F11.5 /REP_DB=WormBase Gene ID /WP=CE10604 /GB=AAK21386.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=vacuolar ATPase G subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059240(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059240 NCBI Caenorhabditis elegans Vacuolar H ATPase family member (vha-10) (vha-10) mRNA, complete cds. 11/11 None
SNAP00000023446 ENSEMBL cdna:SNAP chromosome:CEL140:I:5609591:5610257:-1 11/11 None
GENEFINDER00000023463 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5609591:5616383:-1 11/11 None
F46F11.5.3 ENSEMBL cdna:known chromosome:CEL140:I:5609313:5610741:-1 gene:F46F11.5 11/11 None
F46F11.5.2 ENSEMBL cdna:known chromosome:CEL140:I:5609313:5610279:-1 gene:F46F11.5 11/11 None
F46F11.5.1 ENSEMBL cdna:known chromosome:CEL140:I:5609313:5610273:-1 gene:F46F11.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5609590-5610257(-) 100.0 100.0
Public Domain and Genome References
Gene Title vacuolar ATPase G subunit
Gene Symbol vha-10
Ensembl F46F11.5
Entrez Gene 172216 Entrez gene
SwissProt P91303 EMBL-EBI
WormBase CE10604 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.10969.1.S1_AT ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G isoform 2 cfa
CANINE_2:CFA.10969.1.S1_S_AT ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G isoform 2 cfa
CANINE_2:CFA.10969.1.S2_AT ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G isoform 2 cfa
CANINE:1587041_AT ATPase, H+ transporting, V1 subunit G cfa
U133_X3P:HS.249227.0.S1_3P_AT ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G isoform 2 hs
HG-U133A_2:214762_AT ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G isoform 2 hs
HG-U133_PLUS_2:214762_AT ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G isoform 2 hs
HG-U133A:214762_AT ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G isoform 2 hs
HG-U95AV2:33033_AT ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G isoform 2 hs
MG-U74BV2:111904_AT ATPase, H+ transporting, V1 subunit G isoform 2 mm
MOE430A:1417799_AT ATPase, H+ transporting, V1 subunit G isoform 2 mm
MOUSE430A_2:1417799_AT ATPase, H+ transporting, V1 subunit G isoform 2 mm
MOUSE430_2:1417799_AT ATPase, H+ transporting, V1 subunit G isoform 2 mm
MU19KSUBA:TC19200_AT Atp6g2 mRNA for G2 isoform of V-ATPase subunit mm
RAE230B:1377722_AT ATPase, H+ transporting, V1 subunit G isoform 2 rn
RAT230_2:1377722_AT ATPase, H+ transporting, V1 subunit G isoform 2 rn
Protein Similarities
Method ID Description E-Value
blast AAM15574 Hypothetical protein F46F11.9a [Caenorhabditis elegans] ref|NP_491643.2| F46F11.9a [Caenorhabditis elegans] 0.0
blast AAM15575 Hypothetical protein F46F11.9b [Caenorhabditis elegans] ref|NP_491642.2| F46F11.9b [Caenorhabditis elegans] 0.0
blast CAE68923 Hypothetical protein CBG14902 [Caenorhabditis briggsae] 0.0
blast AAK21386 Vacuolar h atpase protein 10 [Caenorhabditis elegans] ref|NP_491641.1| Vacuolar H ATPase family member (vha-10) [Caenorhabditis elegans] sp|P91303|VATG_CAEEL Probable Vacuolar ATP synthase subunit G (V-ATPase G subunit) (Vacuolar proton pump G subunit) (V-ATPase 13 kDa subunit) 5.0E-64
blast CAE68925 Hypothetical protein CBG14904 [Caenorhabditis briggsae] 6.0E-62
Protein Domains
Database ID Description E-Value
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 1.39999997615814
Pfam IPR005124 EMBL-EBI Vacuolar (H+)-ATPase G subunit 1.3E-56
Pfam IPR005124 EMBL-EBI Vacuolar (H+)-ATPase G subunit 1.3E-56
Sequence
Target Sequence 
>C. ELEGANS:189302_S_AT
atggcctcacaaacccagggaattcagcaacttttggccgctgagaaacgtgccgctgag
aagatcaacgaagctcgtaagagaaagttgcagagaacgaagcaggccaagcaagaggct
caagctgaggtggagaagtacaagcagcaacgcgaggcagagttcaaggcatttgaacaa
caatacttgggaaccaaagaagatattgagagcaagattcgtcgtgatactgaggatcaa
atcagtggaatgaagcaatctgtggctggaaacaagcaagctgtcattgtccgtctcctt
caactcgtttgcgacatcaagccagagctccatcataacttgactctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGCCTCACAAACCCAGGGAATTC 385 53 13 Antisense
GGGAATTCAGCAACTTTTGGCCGCT 665 493 30 Antisense
TTTGGCCGCTGAGAAACGTGCCGCT 259 659 45 Antisense
GAAACGTGCCGCTGAGAAGATCAAC 112 359 57 Antisense
GCAGAGAACGAAGCAGGCCAAGCAA 370 313 102 Antisense
GAAGTACAAGCAGCAACGCGAGGCA 374 335 147 Antisense
CGCGAGGCAGAGTTCAAGGCATTTG 469 255 163 Antisense
GAGAGCAAGATTCGTCGTGATACTG 347 393 220 Antisense
GGAATGAAGCAATCTGTGGCTGGAA 468 531 259 Antisense
ACATCAAGCCAGAGCTCCATCATAA 455 105 326 Antisense
GAGCTCCATCATAACTTGACTCTCC 661 387 337 Antisense
Sequence Source Affymetrix Proprietary Database