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[189297_at] cytochrome P450

Description F42A9.5 /REP_DB=WormBase Gene ID /WP=CE07227 /TR=Q27499 /GB=AAB03160.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 60      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 71      6grp_total > 50      4grp_total > 53      6grp_highest_sub > 03_04      6grp_cgc > 60      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 118
1 2 3 4 range
A 198.8
(1.6)
P 		208.4
(3)
P 		167
(-3)
P 		173.1
(-2.1)
P 		41
B 226.9
(3)
P 		216.7
(2.5)
P 		176.2
(0.4)
P 		108.6
(-3)
P 		118
C 225.4
(3)
P 		182.7
(-0.1)
P 		204.7
(1.5)
P 		142.1
(-3)
P 		83
avr 217
(3) 		202.6
(1.9) 		182.6
(0.3) 		141.3
(-3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 308
1 2 3 4 5 6 range
A 440.7
(3)
P 		238.9
(-2.3)
P 		344.4
(0.5)
P 		372.2
(1.2)
P 		349.3
(0.6)
P 		213.7
(-3)
P 		227
B 304.4
(0.4)
P 		262.9
(-0.4)
P 		436.6
(3)
P 		333.3
(1)
P 		326.4
(0.8)
P 		134
(-3)
P 		303
C 300
(0.2)
P 		336.1
(0.9)
P 		338.2
(0.9)
P 		442.1
(3)
P 		215.7
(-1.5)
P 		139.2
(-3)
P 		303
avr 348.4
(2.1) 		279.3
(0.2) 		373.1
(2.7) 		382.5
(3) 		297.1
(0.7) 		162.3
(-3) 		220
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 72.4
(3)
P 		45.2
(-0.5)
P 		43.7
(-0.7)
P 		25.3
(-3)
P 		47
B 72.4
(3)
P 		53.5
(0.7)
P 		40.8
(-0.8)
P 		23.1
(-3)
P 		49
C 74.6
(3)
P 		52.5
(0.4)
P 		71.4
(2.6)
P 		24
(-3)
P 		51
avr 73.1
(3) 		50.4
(0.2) 		52
(0.4) 		24.1
(-3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 81.2
(0.3)
P 		85
(0.7)
P 		75
(-0.4)
P 		94.4
(1.8)
P 		105.4
(3)
P 		52
(-3)
P 		53
B 105.9
(1.1)
P 		87.5
(-0.3)
P 		106
(1.1)
P 		88.3
(-0.2)
P 		132.6
(3)
P 		50
(-3)
P 		83
C 111.2
(3)
P 		102.6
(2.2)
P 		86.1
(0.7)
P 		88.9
(0.9)
P 		69.8
(-0.8)
P 		46.6
(-3)
P 		65
avr 99.4
(2.6) 		91.7
(1.8) 		89
(1.5) 		90.5
(1.6) 		102.6
(3) 		49.5
(-3) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189297_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9552
Sequence Type Exemplar sequence
Representative Public ID F42A9.5 NCBI
Target Description F42A9.5 /REP_DB=WormBase Gene ID /WP=CE07227 /TR=Q27499 /GB=AAB03160.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069069(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069069 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-33E2) (cyp-33E2) mRNA, complete cds. 11/11 None
SNAP00000042132 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8612957:8614596:1 11/11 None
GENEFINDER00000042145 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8612562:8615853:1 11/11 None
F42A9.5 ENSEMBL cdna:known chromosome:CEL140:IV:8612374:8614629:1 gene:F42A9.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8612567-8614602(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-33E2
Ensembl F42A9.5
Entrez Gene 185653 Entrez gene
SwissProt Q27499 EMBL-EBI
WormBase CE07227 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.8532.1.S1_S_AT similar to cytochrome P450, family 2, subfamily F, polypeptide 1 cfa
CANINE_2:CFAAFFX.8536.1.S1_AT similar to cytochrome P450, family 2, subfamily F, polypeptide 1 cfa
CANINE_2:CFAAFFX.8538.1.S1_AT similar to cytochrome P450, family 2, subfamily F, polypeptide 1 cfa
HG-U95AV2:1517_AT cytochrome P450, family 2, subfamily F, polypeptide 1 hs
HC-G110:1517_AT cytochrome P450, family 2, subfamily F, polypeptide 1 hs
HG-U133_PLUS_2:207913_AT cytochrome P450, family 2, subfamily F, polypeptide 1 hs
HG-U133A:207913_AT cytochrome P450, family 2, subfamily F, polypeptide 1 hs
HG-FOCUS:207913_AT cytochrome P450, family 2, subfamily F, polypeptide 1 hs
HG-U133A_2:207913_AT cytochrome P450, family 2, subfamily F, polypeptide 1 hs
HUGENEFL:J02906_AT cytochrome P450, family 2, subfamily F, polypeptide 1 hs
U133_X3P:G4503224_3P_AT cytochrome P450, family 2, subfamily F, polypeptide 1 hs
MU11KSUBA:M77497_S_AT cytochrome P450, family 2, subfamily f, polypeptide 2 mm
MG-U74AV2:100069_AT cytochrome P450, family 2, subfamily f, polypeptide 2 mm
MOE430A:1448792_A_AT cytochrome P450, family 2, subfamily f, polypeptide 2 mm
MOUSE430A_2:1448792_A_AT cytochrome P450, family 2, subfamily f, polypeptide 2 mm
MOUSE430_2:1448792_A_AT cytochrome P450, family 2, subfamily f, polypeptide 2 mm
RT-U34:AF017393_AT cytochrome P450, family 2, subfamily f, polypeptide 2 rn
RG-U34A:AF017393_AT cytochrome P450, family 2, subfamily f, polypeptide 2 rn
RAE230A:1368608_AT cytochrome P450, family 2, subfamily f, polypeptide 2 rn
RAT230_2:1368608_AT cytochrome P450, family 2, subfamily f, polypeptide 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB03160 Cytochrome p450 family protein 33E2 [Caenorhabditis elegans] ref|NP_501470.1| CYtochrome P450 family member (cyp-33E2) [Caenorhabditis elegans] 0.0
blast AAB03125 Hypothetical protein C49C8.4 [Caenorhabditis elegans] ref|NP_501480.2| CYtochrome P450 family member (cyp-33E1) [Caenorhabditis elegans] 0.0
blast CAE60003 Hypothetical protein CBG03499 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-121
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-145
p450 CP1B_MOUSE Animalia; CYP1B-1; mou; CP1B_MOUSE; Mus musculus; Q64429 1.0E-150
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.5E-31
Pfam IPR001128 EMBL-EBI Cytochrome P450 1.1E-81
Pfam IPR001128 EMBL-EBI Cytochrome P450 6.3E-97
Pfam IPR001128 EMBL-EBI Cytochrome P450 4.3E-112
Pfam IPR001128 EMBL-EBI Cytochrome P450 5.1E-26
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501470.1 3 2-20,59-81,233-255
F42A9.5 3 2-20,59-81,233-255
GENEFINDER00000042145 3 2-20,477-496,535-552
Sequence
Target Sequence 
>C. ELEGANS:189297_AT
ttctttatgtcctccgaaatccagaagtacgccaaaaagtgtatgaagaattggatagag
aaattggaagtgataggattattacaacttctgataaacctaaactcaactatatcaatg
caactattaatgaatcacaacgtcttgccaatcttctcccgatgaatctagccagatcca
caacaaaagacgtggagattgcgggttatcatattaaaaagaatacagtcataattccac
aaatcagtttggttttgtataatccagaaatcttccctgaaccatacgagtttaaaccgg
aaagattcctagaatcggatggaagtttgaaaaaagtcgaagagcttgttccattttcga
ttggaaaacgacagtgtcccggcgaaggacttgccaaaatggaattattattattctttg
ctaatttattcaatagattcgatattcaattacaccagtcaaatccgaatccaagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTTTATGTCCTCCGAAATCCAGA 374 689 998 Antisense
GTCCTCCGAAATCCAGAAGTACGCC 368 475 1006 Antisense
TACAACTTCTGATAAACCTAAACTC 573 639 1080 Antisense
GAATCACAACGTCTTGCCAATCTTC 344 327 1129 Antisense
CAATCTTCTCCCGATGAATCTAGCC 498 181 1146 Antisense
GATGAATCTAGCCAGATCCACAACA 46 413 1158 Antisense
AGACGTGGAGATTGCGGGTTATCAT 269 69 1185 Antisense
TACAGTCATAATTCCACAAATCAGT 348 643 1221 Antisense
AATCCAGAAATCTTCCCTGAACCAT 364 167 1258 Antisense
GATTCGATATTCAATTACACCAGTC 229 433 1433 Antisense
ACACCAGTCAAATCCGAATCCAAGT 679 109 1449 Antisense
Sequence Source Affymetrix Proprietary Database