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[189293_s_at] EGF-like domain

Description H02I12.4 /REP_DB=WormBase Gene ID /WP=CE16161 /TR=O45602 /GB=CAB07218.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=EGF-like domain
Cluster 6grp_total > 68      4grp_total > 02      6grp_cgc > 39      6grp_cgc_sub > Cu      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 28.6
(-0.2)
A 		9.3
(-2.8)
A 		7.8
(-3)
A 		51.6
(3)
A 		44
B 7.2
(-2.1)
A 		9.6
(-1.4)
A 		4.3
(-3)
A 		24.2
(3)
A 		20
C 6.2
(-3)
A 		5.6
(-3)
A 		22.3
(-1.9)
A 		94.4
(3)
A 		89
avr 14
(-2.3) 		8.2
(-3) 		11.5
(-2.6) 		56.7
(3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 223
1 2 3 4 5 6 range
A 11
(-2.7)
A 		3.5
(-3)
A 		20.7
(-2.4)
A 		48.2
(-1.5)
A 		57.9
(-1.2)
A 		181
(3)
P 		178
B 84.6
(-0.6)
A 		41.6
(-1.9)
A 		9.7
(-2.9)
A 		7.2
(-3)
A 		60.1
(-1.4)
A 		199.6
(3)
P 		192
C 74.9
(-1.1)
A 		8.7
(-2.9)
A 		5.1
(-3)
A 		9.3
(-2.9)
A 		24.9
(-2.5)
A 		226.4
(3)
P 		221
avr 56.8
(-1.6) 		17.9
(-2.8) 		11.8
(-3) 		21.6
(-2.7) 		47.6
(-1.9) 		202.3
(3) 		191
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 10.4
(3)
A 		2
(-3)
A 		2
(-3)
A 		7.6
(1)
A 		8
B 2.3
(-1.1)
A 		2.4
(-1)
A 		1
(-3)
A 		5.1
(3)
A 		4
C 2.1
(-2.8)
A 		1.6
(-3)
A 		7.8
(-0.4)
A 		15.9
(3)
A 		14
avr 4.9
(-0.7) 		2
(-3) 		3.6
(-1.7) 		9.5
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 2
(-2.9)
A 		1.2
(-3)
A 		4.5
(-2.5)
A 		12.2
(-1.5)
A 		17.5
(-0.7)
A 		44.1
(3)
P 		43
B 29.4
(-0.7)
A 		13.9
(-2)
A 		2.3
(-3)
A 		1.9
(-3)
A 		24.4
(-1.1)
A 		74.4
(3)
P 		73
C 27.8
(-0.9)
A 		2.7
(-2.9)
A 		1.3
(-3)
A 		1.9
(-3)
A 		8
(-2.5)
A 		75.8
(3)
P 		75
avr 19.7
(-1.4) 		5.9
(-2.7) 		2.7
(-3) 		5.3
(-2.7) 		16.6
(-1.7) 		64.8
(3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189293_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9353
Sequence Type Exemplar sequence
Representative Public ID H02I12.4 NCBI
Target Description H02I12.4 /REP_DB=WormBase Gene ID /WP=CE16161 /TR=O45602 /GB=CAB07218.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=EGF-like domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069747(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069747 NCBI Caenorhabditis elegans Delta/Serrate/Lag-2 domain family member (dsl-6) (dsl-6) mRNA, complete cds. 11/11 None
SNAP00000036322 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11396817:11398262:-1 11/11 None
GENEFINDER00000036328 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11396817:11397891:-1 11/11 None
H02I12.4 ENSEMBL cdna:known chromosome:CEL140:IV:11396759:11398267:-1 gene:H02I12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11396822-11398361(-) 100.0 100.0
Public Domain and Genome References
Gene Title EGF-like domain
Gene Symbol dsl-6
Ensembl H02I12.4
Entrez Gene 178063 Entrez gene
SwissProt O45602 EMBL-EBI
WormBase CE36597 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7154 cell communication inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07218 Hypothetical protein H02I12.4 [Caenorhabditis elegans] ref|NP_502148.2| Delta/Serrate/Lag-2 domain family member (dsl-6) [Caenorhabditis elegans] 1.0E-176
blast CAB07218 Hypothetical protein H02I12.4 [Caenorhabditis elegans] ref|NP_502148.2| Delta/Serrate/Lag-2 domain family member (dsl-6) [Caenorhabditis elegans] 1.0E-172
blast CAB07218 Hypothetical protein H02I12.4 [Caenorhabditis elegans] ref|NP_502148.2| Delta/Serrate/Lag-2 domain family member (dsl-6) [Caenorhabditis elegans] 1.0E-124
blast CAA97804 Hypothetical protein F58B3.8 [Caenorhabditis elegans] ref|NP_502191.1| Delta/Serrate/Lag-2 domain family member (dsl-5) [Caenorhabditis elegans] 9.0E-56
blast CAA97804 Hypothetical protein F58B3.8 [Caenorhabditis elegans] ref|NP_502191.1| Delta/Serrate/Lag-2 domain family member (dsl-5) [Caenorhabditis elegans] 5.0E-53
blast CAA97804 Hypothetical protein F58B3.8 [Caenorhabditis elegans] ref|NP_502191.1| Delta/Serrate/Lag-2 domain family member (dsl-5) [Caenorhabditis elegans] 3.0E-36
blast AAK68460 Delta/serrate/lag-2 domain protein 3 [Caenorhabditis elegans] ref|NP_500108.1| Delta/Serrate/Lag-2 domain family member (dsl-3) [Caenorhabditis elegans] 6.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR006209 EMBL-EBI EGF-like domain 7.8E-7
Pfam IPR001774 EMBL-EBI Delta/Serrate/lag-2 (DSL) protein 1.6E-15
Pfam IPR006209 EMBL-EBI EGF-like domain 7.8E-7
Pfam IPR001774 EMBL-EBI Delta/Serrate/lag-2 (DSL) protein 8.4E-14
Pfam IPR006209 EMBL-EBI EGF-like domain 7.8E-7
Pfam IPR001774 EMBL-EBI Delta/Serrate/lag-2 (DSL) protein 1.6E-15
Sequence
Target Sequence 
>C. ELEGANS:189293_S_AT
tccacgtgttggattgatctccccaattcaagttacacgtacatttgatggtttacggat
tatcattgaatgtgatgaaaactggttcggtgaaaaatgtgatgttttctgcgataacat
tgaacaaagactacatggaaagcgttgcaattaccgtggagttccaagttgtttaaatat
gtttactggagcaggatgtatgaaaatgattactccgatggactgctcatgcaagaacaa
tggaaaatgtgttgactccgctgaaacgccaatttgtgaatgtacgagaggttttaaggg
agacacgtgtgaggaaatgcacgaaactataaaagcagctattaatgtccaacaggaaga
aatcggatgcggtggatcaattcataatagtattatggagaatttagttctaaatcagat
gagagaagttaaacaagatacaatgtttcaacttcttagaagcttggtaggagcgatctt
tggggctatttctagtgttttaccaattgatggaacactcggaaatgcagcctgtaataa
ttctcttgtggatagtgtttctgaattggatggaagtggcgtattccctgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCACGTGTTGGATTGATCTCCCCA 86 589 396 Antisense
GATCTCCCCAATTCAAGTTACACGT 342 419 411 Antisense
AAGCGTTGCAATTACCGTGGAGTTC 8 151 535 Antisense
GATTACTCCGATGGACTGCTCATGC 676 429 603 Antisense
AAATGTGTTGACTCCGCTGAAACGC 224 121 640 Antisense
ACTCCGCTGAAACGCCAATTTGTGA 617 97 650 Antisense
AAAGCAGCTATTAATGTCCAACAGG 320 123 727 Antisense
GCGATCTTTGGGGCTATTTCTAGTG 415 291 868 Antisense
GGAACACTCGGAAATGCAGCCTGTA 247 529 907 Antisense
GCAGCCTGTAATAATTCTCTTGTGG 402 313 922 Antisense
GGATGGAAGTGGCGTATTCCCTGAG 449 513 963 Antisense
Sequence Source Affymetrix Proprietary Database