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[189292_at] cytochrome P450

Description K06B9.1 /REP_DB=WormBase Gene ID /WP=CE07339 /TR=Q21262 /GB=AAA93451.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 67      4grp_high_8_8 > 06      6grp_highest_nonorm > 93      6grp_total > 68      4grp_total > 02      6grp_highest_sub > Da      6grp_cgc > 38      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3956
1 2 3 4 range
A 95.4
(-3)
P 		216.4
(-2.8)
P 		129.3
(-2.9)
P 		4051
(3)
P 		3956
B 97
(-3)
P 		207.5
(-2.8)
P 		96
(-3)
P 		3624.1
(3)
P 		3528
C 274
(-2.8)
P 		292
(-2.7)
M 		144.2
(-3)
P 		3477.7
(3)
P 		3334
avr 155.5
(-2.9) 		238.6
(-2.8) 		123.2
(-3) 		3717.6
(3) 		3594

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1035
1 2 3 4 5 6 range
A 248
(-2.9)
P 		378
(-1.9)
P 		364.6
(-2)
P 		423.2
(-1.5)
P 		238.5
(-3)
P 		995.1
(3)
P 		757
B 465.5
(-1.4)
P 		193
(-3)
P 		218.3
(-2.9)
P 		276.5
(-2.5)
P 		431.1
(-1.6)
P 		1227.9
(3)
P 		1035
C 646.3
(1.9)
P 		268.3
(-2.7)
P 		328.4
(-2)
P 		247.9
(-3)
P 		314.8
(-2.2)
P 		735.9
(3)
P 		488
avr 453.3
(-1.5) 		279.8
(-3) 		303.8
(-2.8) 		315.9
(-2.7) 		328.1
(-2.6) 		986.3
(3) 		707
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 750
1 2 3 4 range
A 34.7
(-3)
P 		47
(-2.9)
P 		33.8
(-3)
P 		593
(3)
P 		559
B 30.9
(-2.9)
P 		51.3
(-2.8)
P 		22.2
(-3)
P 		772.3
(3)
P 		750
C 90.7
(-2.5)
P 		83.9
(-2.6)
M 		50.3
(-3)
P 		586.4
(3)
P 		536
avr 52.1
(-2.8) 		60.7
(-2.8) 		35.4
(-3) 		650.6
(3) 		615

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 412
1 2 3 4 5 6 range
A 45.7
(-3)
P 		134.4
(-0.3)
P 		79.4
(-2)
P 		107.3
(-1.1)
P 		72
(-2.2)
P 		242.3
(3)
P 		197
B 162
(-1.4)
P 		64.2
(-2.8)
P 		53
(-3)
P 		73.2
(-2.7)
P 		175.2
(-1.2)
P 		457.9
(3)
P 		405
C 239.5
(2.8)
P 		81.9
(-2)
P 		83.6
(-2)
P 		49.8
(-3)
P 		101.8
(-1.4)
P 		246.3
(3)
P 		197
avr 149.1
(-1.1) 		93.5
(-2.5) 		72
(-3) 		76.8
(-2.9) 		116.3
(-1.9) 		315.5
(3) 		244
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189292_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11067
Sequence Type Exemplar sequence
Representative Public ID K06B9.1 NCBI
Target Description K06B9.1 /REP_DB=WormBase Gene ID /WP=CE07339 /TR=Q21262 /GB=AAA93451.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068111(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068111 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-25A6) (cyp-25A6) mRNA, complete cds. 11/11 None
SNAP00000011536 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4197460:4198358:1 10/11 None
GENEFINDER00000011546 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4197460:4199158:1 10/11 None
K06B9.1 ENSEMBL cdna:known chromosome:CEL140:IV:4197460:4198358:1 gene:K06B9.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028392 8/11 Cross Hyb Matching Probes None
GENEFINDER00000028404 8/11 Cross Hyb Matching Probes None
C36A4.3 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4197465-4198364(+) 98.01 100.0
chrIII:3838574-3839454(-) 96.02 96.02
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-25A6
Ensembl K06B9.1
Entrez Gene 187059 Entrez gene
SwissProt Q21262 EMBL-EBI
WormBase CE07339 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA93451 Cytochrome p450 family protein 25A6 [Caenorhabditis elegans] ref|NP_500512.1| CYtochrome P450 family member (cyp-25A6) [Caenorhabditis elegans] 1.0E-131
blast AAA93451 Cytochrome p450 family protein 25A6 [Caenorhabditis elegans] ref|NP_500512.1| CYtochrome P450 family member (cyp-25A6) [Caenorhabditis elegans] 1.0E-119
blast A88414 protein C36A4.3 [imported] - Caenorhabditis elegans 1.0E-117
blast AAA93451 Cytochrome p450 family protein 25A6 [Caenorhabditis elegans] ref|NP_500512.1| CYtochrome P450 family member (cyp-25A6) [Caenorhabditis elegans] 1.0E-117
blast A88414 protein C36A4.3 [imported] - Caenorhabditis elegans 1.0E-114
blast CAA91267 Hypothetical protein C36A4.2 [Caenorhabditis elegans] ref|NP_497776.2| CYtochrome P450 family member (cyp-25A2) [Caenorhabditis elegans] 2.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 2.5E-21
Pfam IPR001128 EMBL-EBI Cytochrome P450 4.4E-27
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500512.1 1 4-23
K06B9.1 1 4-23
SNAP00000011536 1 4-23
GENEFINDER00000011546 1 4-23
Sequence
Target Sequence 
>C. ELEGANS:189292_AT
ggttacgatgattcttatgattggtacaacaagcttcacaaacaatttggcgaaactttt
ggaatctactttggcacccagctcaacataaacattaccaatgaggaagatattaaagaa
gtttttatcaaaaatttctcgaatttttcggacagaactccaccaccaattattgaagat
aataaactcaaagaatcgttgctccaaaacacatacgaatctggatggaagcatactcga
tctgcaatcgctccgattttttcaacgggaaaaatgaaggcaatgcatgagacaattcat
tcgaaagttgacctgttcctggagattttgaaggaaaaagcaagctccgggcagaaatgg
gatatttatgagttagttcaaaaacttttgaatgattcggatatttattatttcagcgat
ttccaaggtct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTACGATGATTCTTATGATTGGT 265 507 187 Antisense
CAACAAGCTTCACAAACAATTTGGC 214 179 213 Antisense
TTTGGAATCTACTTTGGCACCCAGC 245 665 244 Antisense
GCACCCAGCTCAACATAAACATTAC 316 315 260 Antisense
GAATTTTTCGGACAGAACTCCACCA 169 307 327 Antisense
TAAACTCAAAGAATCGTTGCTCCAA 218 639 369 Antisense
GTTGCTCCAAAACACATACGAATCT 464 439 384 Antisense
CGATCTGCAATCGCTCCGATTTTTT 510 249 424 Antisense
CAATCGCTCCGATTTTTTCAACGGG 36 181 431 Antisense
AAAGTTGACCTGTTCCTGGAGATTT 376 123 490 Antisense
ATTATTTCAGCGATTTCCAAGGTCT 538 13 593 Antisense
Sequence Source Affymetrix Proprietary Database