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[189289_s_at] protein kinase

Description F28C10.3 /REP_DB=WormBase Gene ID /WP=CE07156 /TR=Q19858 /GB=AAB00607.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_highest_10_10 > 74      6grp_high1_10_10 > 47      4grp_highest_8_8 > 08      4grp_high_8_8 > 44      6grp_highest_nonorm > 90      6grp_total > 47      4grp_total > 52      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 160.8
(-1.6)
P 		164.1
(-0.9)
P 		182.5
(3)
P 		153.9
(-3)
P 		29
B 152.6
(-0.3)
P 		186.6
(3)
P 		137.6
(-1.7)
P 		124.1
(-3)
P 		63
C 160.2
(2.6)
P 		163.5
(3)
P 		148.9
(1.2)
P 		114
(-3)
P 		50
avr 157.9
(1) 		171.4
(3) 		156.3
(0.8) 		130.7
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 163.5
(1.3)
P 		146.5
(0.4)
P 		117.8
(-1)
P 		96.6
(-2.1)
P 		78.7
(-3)
P 		197.8
(3)
P 		119
B 212.4
(3)
P 		124.2
(-1.3)
P 		112.9
(-1.9)
P 		102.3
(-2.4)
P 		90.5
(-3)
P 		174.6
(1.1)
P 		122
C 185.8
(2.3)
P 		133.5
(-1.3)
P 		129.3
(-1.6)
P 		123.2
(-2)
P 		109.4
(-3)
P 		195.2
(3)
P 		86
avr 187.2
(2.9) 		134.7
(-0.4) 		120
(-1.3) 		107.4
(-2.1) 		92.9
(-3) 		189.2
(3) 		96
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 58.5
(3)
P 		35.6
(-0.8)
P 		47.7
(1.2)
P 		22.5
(-3)
P 		36
B 48.7
(3)
P 		46.1
(2.3)
P 		31.9
(-1.5)
P 		26.4
(-3)
P 		22
C 53
(3)
P 		47
(1.9)
P 		51.9
(2.8)
P 		19.2
(-3)
P 		34
avr 53.4
(3) 		42.9
(0.9) 		43.8
(1.1) 		22.7
(-3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 30.1
(-1.7)
P 		52.1
(3)
P 		25.7
(-2.6)
P 		24.5
(-2.9)
P 		23.8
(-3)
P 		48.2
(2.2)
P 		28
B 73.9
(3)
P 		41.4
(-1.2)
P 		27.4
(-3)
P 		27.1
(-3)
P 		36.8
(-1.8)
P 		65.1
(1.9)
P 		47
C 68.9
(3)
P 		40.7
(-0.8)
P 		32.9
(-1.9)
P 		24.8
(-3)
P 		35.4
(-1.6)
P 		65.3
(2.5)
P 		44
avr 57.6
(2.7) 		44.7
(0.4) 		28.7
(-2.4) 		25.5
(-3) 		32
(-1.9) 		59.5
(3) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189289_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18519
Sequence Type Exemplar sequence
Representative Public ID F28C10.3 NCBI
Target Description F28C10.3 /REP_DB=WormBase Gene ID /WP=CE07156 /TR=Q19858 /GB=AAB00607.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075713(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075713 NCBI Caenorhabditis elegans F28C10.3 (F28C10.3) mRNA, complete cds. 11/11 None
SNAP00000036535 ENSEMBL cdna:SNAP chromosome:CEL140:X:532287:535748:-1 10/11 None
GENEFINDER00000036543 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:532287:540901:-1 10/11 None
F28C10.3 ENSEMBL cdna:known chromosome:CEL140:X:531886:535750:-1 gene:F28C10.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:532150-535748(-) 92.71 92.71
Public Domain and Genome References
Gene Title protein kinase
Ensembl F28C10.3
Entrez Gene 180405 Entrez gene
SwissProt Q19858 EMBL-EBI
WormBase CE07156 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB00607 Hypothetical protein F28C10.3 [Caenorhabditis elegans] ref|NP_508114.1| F28C10.3 [Caenorhabditis elegans] 0.0
blast AAB00607 Hypothetical protein F28C10.3 [Caenorhabditis elegans] ref|NP_508114.1| F28C10.3 [Caenorhabditis elegans] 1.0E-176
blast AAB00607 Hypothetical protein F28C10.3 [Caenorhabditis elegans] ref|NP_508114.1| F28C10.3 [Caenorhabditis elegans] 1.0E-170
blast AAB00608 Hypothetical protein F28C10.4 [Caenorhabditis elegans] ref|NP_508115.1| F28C10.4 [Caenorhabditis elegans] 1.0E-126
blast CAE63652 Hypothetical protein CBG08151 [Caenorhabditis briggsae] 3.0E-93
blast CAE63652 Hypothetical protein CBG08151 [Caenorhabditis briggsae] 5.0E-93
blast CAE63652 Hypothetical protein CBG08151 [Caenorhabditis briggsae] 7.0E-87
blast XP_699952 PREDICTED: similar to ribosomal protein S6 kinase, 90kDa, polypeptide 2 isoform a [Danio rerio] 1.0E-52
Protein Families
Method ID Description E-Value
hanks 1.4.5 AGC group; AGC IV GPCR kinases; IT11 3.0E-63
hanks 1.4.4 AGC group; AGC IV GPCR kinases; GRK5 2.0E-63
hanks 1.2.13 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; PKC1 3.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.0E-53
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 8.9E-5
Pfam IPR000719 EMBL-EBI Protein kinase 1.0E-49
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 8.9E-5
Sequence
Target Sequence 
>C. ELEGANS:189289_S_AT
ttcctgcgactggtgggctgttggaatcacttattgcgatatgcgttccgacaaggctgt
atttgatggcgctgacagcaaagagtactctgatagcactgcaaagaagcgcccacgact
tccaaaagttttaagcccacgtgagagaggttttgtcaacaaactgattgtccgcgatcc
gacgcaacgtcttggcaacggaccggacggaactgcaactgtcaaggcacatgatatgtt
caaaggagttgtctgggaggatgtgctcgccaagaannnnnnnnnnnnnnnnnnnnnnnn
nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn
nnacaccctgtcccactttactggactgatattgacttcacggacccatccctgtgcaac
aactagtcatccaacaagaatggatttcaatatcattttctattgccatgccaagt

BLASTn GenBank NR
Note: "n"'s represent regions that are not probed by the probe sequences.
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCCTGCGACTGGTGGGCTGTTGGA 461 699 630 Antisense
TGCGATATGCGTTCCGACAAGGCTG 365 583 664 Antisense
ATAGCACTGCAAAGAAGCGCCCACG 532 21 722 Antisense
AAGCCCACGTGAGAGAGGTTTTGTC 282 147 762 Antisense
CAACAAACTGATTGTCCGCGATCCG 667 189 786 Antisense
GATCCGACGCAACGTCTTGGCAACG 293 417 805 Antisense
GACCGGACGGAACTGCAACTGTCAA 425 383 830 Antisense
GTTGTCTGGGAGGATGTGCTCGCCA 614 437 877 Antisense
CCCTGTCCCACTTTACTGGACTGAT 317 259 995 Antisense
CTGATATTGACTTCACGGACCCATC 255 237 1015 Antisense
ATCATTTTCTATTGCCATGCCAAGT 350 31 1081 Antisense
Sequence Source Affymetrix Proprietary Database