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[189287_at] EF hand protein

Description F08B6.3 /REP_DB=WormBase Gene ID /WP=CE20657 /TR=Q9TYS8 /GB=AAC78237.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=EF hand protein
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 50      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_highest_nonorm > 42      6grp_total > 40      4grp_total > 37      6grp_highest_sub > 03_04      6grp_cgc > 83      6grp_high1_sub > 03_04      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 295
1 2 3 4 range
A 806.1
(3)
P 		792.7
(2.7)
P 		543.2
(-3)
P 		588.6
(-2)
P 		263
B 724.8
(3)
P 		625.9
(-0.2)
P 		536.6
(-3)
P 		594.2
(-1.2)
P 		188
C 754.3
(3)
P 		712
(2)
P 		510.9
(-3)
P 		564
(-1.7)
P 		243
avr 761.7
(3) 		710.2
(1.7) 		530.2
(-3) 		582.3
(-1.6) 		232

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 702
1 2 3 4 5 6 range
A 885.9
(1)
P 		781
(0.1)
P 		1124.6
(3)
P 		1053.6
(2.4)
P 		527.4
(-2.1)
P 		422.3
(-3)
P 		702
B 685.1
(-0.4)
P 		781.5
(0.8)
P 		966.3
(3)
P 		750.5
(0.4)
P 		563.5
(-1.9)
P 		468.4
(-3)
P 		498
C 758.9
(0.6)
P 		875.2
(2.1)
P 		938.4
(3)
P 		941.7
(3)
P 		536.5
(-2.3)
P 		484.7
(-3)
P 		457
avr 776.6
(0.5) 		812.6
(0.9) 		1009.8
(3) 		915.3
(2) 		542.5
(-2.1) 		458.5
(-3) 		551
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 207
1 2 3 4 range
A 293.5
(3)
P 		172.1
(-0.5)
P 		142
(-1.4)
P 		86.2
(-3)
P 		207
B 231.1
(3)
P 		154.6
(-1.3)
P 		124.3
(-3)
P 		126.6
(-2.9)
P 		107
C 249.7
(3)
P 		204.7
(1.3)
P 		178.1
(0.2)
P 		95.1
(-3)
P 		155
avr 258.1
(3) 		177.1
(-0.1) 		148.1
(-1.2) 		102.6
(-3) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 179
1 2 3 4 5 6 range
A 163.3
(-0.9)
P 		277.8
(3)
P 		244.9
(1.9)
P 		267.2
(2.6)
P 		159.1
(-1.1)
P 		102.8
(-3)
P 		175
B 238.4
(1.5)
P 		260.1
(3)
P 		234.6
(1.2)
P 		198.8
(-1.3)
P 		228.9
(0.8)
P 		174.7
(-3)
P 		85
C 281.3
(3)
P 		267
(2.3)
P 		238.8
(0.9)
P 		189.4
(-1.6)
P 		173.6
(-2.4)
P 		162.3
(-3)
P 		119
avr 227.7
(1) 		268.3
(3) 		239.4
(1.6) 		218.5
(0.5) 		187.2
(-1) 		146.6
(-3) 		122
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189287_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2455
Sequence Type Exemplar sequence
Representative Public ID F08B6.3 NCBI
Target Description F08B6.3 /REP_DB=WormBase Gene ID /WP=CE20657 /TR=Q9TYS8 /GB=AAC78237.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=EF hand protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059535(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059535 NCBI Caenorhabditis elegans F08B6.3 (F08B6.3) mRNA, complete cds. 11/11 None
SNAP00000014329 ENSEMBL cdna:SNAP chromosome:CEL140:I:6766372:6768185:-1 10/11 None
GENEFINDER00000014334 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6766372:6768301:-1 10/11 None
F08B6.3 ENSEMBL cdna:known chromosome:CEL140:I:6766372:6768301:-1 gene:F08B6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6766300-6768216(-) 98.3 100.0
Public Domain and Genome References
Gene Title EF hand protein
Ensembl F08B6.3
Entrez Gene 184174 Entrez gene
SwissProt Q9TYS8 EMBL-EBI
WormBase CE34292 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC78237 Hypothetical protein F08B6.3 [Caenorhabditis elegans] ref|NP_491936.2| F08B6.3 [Caenorhabditis elegans] 1.0E-173
blast AAC78237 Hypothetical protein F08B6.3 [Caenorhabditis elegans] ref|NP_491936.2| F08B6.3 [Caenorhabditis elegans] 1.0E-161
blast AAC78237 Hypothetical protein F08B6.3 [Caenorhabditis elegans] ref|NP_491936.2| F08B6.3 [Caenorhabditis elegans] 1.0E-148
blast CAE60447 Hypothetical protein CBG04055 [Caenorhabditis briggsae] 4.0E-96
blast CAE60447 Hypothetical protein CBG04055 [Caenorhabditis briggsae] 4.0E-93
blast CAE60447 Hypothetical protein CBG04055 [Caenorhabditis briggsae] 2.0E-91
blast EAL33018 GA16367-PA [Drosophila pseudoobscura] 2.0E-45
blast EAL33018 GA16367-PA [Drosophila pseudoobscura] 7.0E-45
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 5.6E-4
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.041
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.0030
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.048
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.31
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 5.6E-4
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.041
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.0030
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.048
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.31
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.0030
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.048
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.31
Sequence
Target Sequence 
>C. ELEGANS:189287_AT
aaatacgcttttttctctcgtagataggaaacatgtatcgtgggaagaatacttggccga
ttcatttcctgacgaagagttacataacaaggaagaagaatcacttattgcacaggataa
aatgtatttcaaacaagctgacgaagacaatgatggaaaattaaatctggaagaactggc
aagttttcttaacccggagcatcatccacatatgcatccggttctaattgctgtaacgct
gctagaaaaagaccagaatggagatggagcgatcgaagagaaggaatttttgggagaact
tgatgaacaaagaggcagtgaatggtataaagttgaggtggagagattccgtacagttta
tgacaaaaacaaagacggaaaactggccggcgacgagctgaccgattggcttctcgttga
tggaacaacagctggatcttatgaagctgaaagtcttctaacaaattcagatgatgataa
agatggacaattgtcttatgaagaaattgtgaagcatcatgctctttttgccaaaactga
agctgctcaagaagccgatcatctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATACGCTTTTTTCTCTCGTAGAT 25 117 360 Antisense
ATACTTGGCCGATTCATTTCCTGAC 72 25 408 Antisense
TGGCAAGTTTTCTTAACCCGGAGCA 202 545 536 Antisense
TAACCCGGAGCATCATCCACATATG 187 639 549 Antisense
TCCACATATGCATCCGGTTCTAATT 13 589 564 Antisense
GGTTCTAATTGCTGTAACGCTGCTA 298 507 579 Antisense
GGAGAGATTCCGTACAGTTTATGAC 582 517 699 Antisense
ACGGAAAACTGGCCGGCGACGAGCT 332 93 734 Antisense
CGAGCTGACCGATTGGCTTCTCGTT 126 251 753 Antisense
GAAGCATCATGCTCTTTTTGCCAAA 416 341 870 Antisense
AGCTGCTCAAGAAGCCGATCATCTG 398 81 900 Antisense
Sequence Source Affymetrix Proprietary Database